Optimization of Methods for Articular Cartilage Surface Tissue Engineering: Cell Density and Transforming Growth Factor Beta Are Critical for Self-Assembly and Lubricin Secretion

The response of secondary human laryngeal epithelial cells to transforming growth factor-beta (TGF-beta) was investigated, and this response was compared with that of epithelial cells derived from virally induced laryngeal papillomas. In most cases, both normal laryngeal epithelial cells and those derived from laryngeal papillomas exhibited growth inhibition in response to 10 ng ml-1 TGF-beta. Response was not a function of cell density or proliferation rate when cells were in a low-calcium medium, but was reduced in high calcium. Using keratinocytes derived from several different tissue explants, we found that cells grown from different explants show marked variation in response to TGF-beta.

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Mechanical stress contributes to osteoarthritis development through the activation of transforming growth factor beta 1 (TGF-β1)

Bone and Joint Research ◽

10.1302/2046-3758.711.bjr-2018-0057.r1 ◽

2018 ◽

Vol 7 (11) ◽

pp. 587-594 ◽

Cited By ~ 8

Author(s):

R-K. Zhang ◽

G-W. Li ◽

C. Zeng ◽

C-X. Lin ◽

L-S. Huang ◽

...

Keyword(s):

Articular Cartilage ◽

Growth Factor ◽

Cyclic Loading ◽

Mechanical Stress ◽

Transforming Growth Factor Beta ◽

Transforming Growth Factor ◽

Cartilage Degradation ◽

Growth Factor Beta ◽

Tgf Β1 ◽

Cultured Chondrocytes

Objectives The role of mechanical stress and transforming growth factor beta 1 (TGF-β1) is important in the initiation and progression of osteoarthritis (OA). However, the underlying molecular mechanisms are not clearly known. Methods In this study, TGF-β1 from osteoclasts and knee joints were analyzed using a co-cultured cell model and an OA rat model, respectively. Five patients with a femoral neck fracture (four female and one male, mean 73.4 years (68 to 79)) were recruited between January 2015 and December 2015. Results showed that TGF-β1 was significantly upregulated in osteoclasts by cyclic loading in a time- and dose-dependent mode. The osteoclasts were subjected to cyclic loading before being co-cultured with chondrocytes for 24 hours. Results A significant decrease in the survival rate of co-cultured chondrocytes was found. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labelling (TUNEL) assay demonstrated that mechanical stress-induced apoptosis occurred significantly in co-cultured chondrocytes but administration of the TGF-β1 receptor inhibitor, SB-505124, can significantly reverse these effects. Abdominal administration of SB-505124 can attenuate markedly articular cartilage degradation in OA rats. Conclusion Mechanical stress-induced overexpression of TGF-β1 from osteoclasts is responsible for chondrocyte apoptosis and cartilage degeneration in OA. Administration of a TGF-β1 inhibitor can inhibit articular cartilage degradation. Cite this article: R-K. Zhang, G-W. Li, C. Zeng, C-X. Lin, L-S. Huang, G-X. Huang, C. Zhao, S-Y. Feng, H. Fang. Mechanical stress contributes to osteoarthritis development through the activation of transforming growth factor beta 1 (TGF-β1). Bone Joint Res 2018;7:587–594. DOI: 10.1302/2046-3758.711.BJR-2018-0057.R1.

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Inhibition of proteoglycan synthesis by transforming growth factor beta in anatomically intact articular cartilage of murine patellae.

Annals of the Rheumatic Diseases ◽

10.1136/ard.51.5.643 ◽

1992 ◽

Vol 51 (5) ◽

pp. 643-647 ◽

Cited By ~ 26

Author(s):

P M van der Kraan ◽

E L Vitters ◽

W B van den Berg

Keyword(s):

Articular Cartilage ◽

Growth Factor ◽

Transforming Growth Factor Beta ◽

Transforming Growth Factor ◽

Proteoglycan Synthesis ◽

Growth Factor Beta

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In vitro Response of Articular Cartilage from Mature Mice to Human Transforming Growth Factor Beta

Cells Tissues Organs ◽

10.1159/000147575 ◽

1994 ◽

Vol 149 (3) ◽

pp. 185-194 ◽

Cited By ~ 11

Author(s):

E. Livne

Keyword(s):

Articular Cartilage ◽

Growth Factor ◽

Transforming Growth Factor Beta ◽

Transforming Growth Factor ◽

In Vitro Response ◽

Growth Factor Beta

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The Expression of IL-1, IL-6, and TGF-β in the Synovial Fluid of Horses with Surgically-Induced Transient Synovitis

Veterinary and Comparative Orthopaedics and Traumatology ◽

10.1055/s-0038-1632536 ◽

1998 ◽

Vol 11 (03) ◽

pp. 141-145 ◽

Cited By ~ 1

Author(s):

S. M. Barber ◽

J. R. Gordon ◽

Christine Theoret

Keyword(s):

Articular Cartilage ◽

Growth Factor ◽

Synovial Fluid ◽

Transforming Growth Factor Beta ◽

Interleukin 6 ◽

Transforming Growth Factor ◽

Interleukin 1 ◽

Transient Synovitis ◽

Growth Factor Beta ◽

Surgically Induced

SummaryThis study investigates the presence of various cytokines in the synovial fluid of three horses with surgicallyinduced synovitis in one antebrachiocarpal joint. Synovial fluids were sampled from experimental and control joints, preoperatively and at two and 30 days post-operatively. Samples were analyzed for interleukin-1 and interleukin- 6 bioactivities with a bioassay, and for transforming growth factor-beta protein by ELISA. Peak cytokine levels were detected on day two post-synovectomy, and returned to pre-operative levels by 30 days post-synovectomy (Interleukin-6 in treated joints: day #0; 0.53 ±0.2, day #2; 9.8 ± 0.7, day #30; 0.4 ± 0.2. Transforming growth factorbeta in treated joints: day #0; 314 ± 69, day #2; 1101 ± 325, day #30; 321 ± 101). Arthrocentesis alone (i.e. control joints) caused no increase in interleukin- 1, interleukin-6 and transforming growth factor-beta levels. Interleukin-1 and interleukin-6 are thought to both prevent anabolic and enhance catabolic effects within articular cartilage matrices, whereas it has been proposed that transforming growth factor-beta may have a protective effect on the articular cartilage. This study is the first to document elevated transforming growth factor-beta levels in equine joints.This study documents elevated levels of transforming growth factor-beta, interleukin-1 and interleukin-6 in the joints of horses with surgically-induced transient synovitis.

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