Bone Marrow-Derived Mesenchymal Stem Cells as Autologous Therapy in Dogs with Naturally Occurring Intervertebral Disc Disease: Feasibility, Safety, and Preliminary Results

2017 ◽  
Vol 23 (11) ◽  
pp. 643-651 ◽  
Author(s):  
Frank Steffen ◽  
Lucas Adam Smolders ◽  
Anne Marie Roentgen ◽  
Alessandro Bertolo ◽  
Jivko Stoyanov
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Intervertebral Disc ◽  
Intervertebral Disc Disease ◽  
Disc Disease ◽  
Preliminary Results ◽  
Naturally Occurring ◽  
Autologous Therapy

Response of Mesenchymal Stem Cells and Intervertebral Disc Cells to Inflammatory Challenge

2011 ◽  
Author(s):  
Neena Rajan ◽  
Nathaniel Stetson ◽  
Passquale Razzano ◽  
Mitchell Levine ◽  
Daniel Grande ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Intervertebral Disc ◽  
Disc Disease ◽  
Clinical Model ◽  
Injury Model ◽  
Tnf Α ◽  
Disc Cells ◽  
Ivd Degeneration ◽  
Pro Inflammatory Cytokines

Human intervertebral disc (IVD) degeneration is accompanied by elevated levels of pro-inflammatory cytokines, particularly IL-1β and TNF-α [1–3]. Cytokine secretion by disc cells increases catabolic breakdown of the tissue, resulting in a positive feedback of disc integrity loss and further inflammation [4–6]. A recent study by our group has shown that severity of degeneration in an injury model can influence the therapeutic effect of cell based repair, such as treatment with mesenchymal stem cells (MSCs) [7]. The goal of this study is to measure the response of MSCs to inflammatory challenge, and to compare this response to that of differentiated disc cells from the nucleus pulposus (NP), annulus fibrosis (AF) and end plate (EP). In this study, we investigated the effects of lipopolysaccharide (LPS) on intervertebral disc cells and MSCs viability, pro-inflammatory cytokine expression and extracellular matrix (ECM) expression. LPS is an endotoxin that induces strong immune responses in animal tissue and hence widely used as a pre-clinical model of inflammation. This approach provides an opportunity to study broad aspects of the physiological inflammatory process observed in degenerative disc disease.

scholarly journals Beneficial Effects of Autologous Bone Marrow-Derived Mesenchymal Stem Cells in Naturally Occurring Tendinopathy

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e75697 ◽  
Author(s):  
Roger Kenneth Whealands Smith ◽  
Natalie Jayne Werling ◽  
Stephanie Georgina Dakin ◽  
Rafiqul Alam ◽  
Allen E. Goodship ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Autologous Bone Marrow ◽  
Beneficial Effects ◽  
Naturally Occurring ◽  
Autologous Bone
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