Three-Dimensional Cultures of Human Subcutaneous Adipose Tissue-Derived Progenitor Cells Based on RAD16-I Self-Assembling Peptide

2016 ◽  
Vol 22 (2) ◽  
pp. 113-124 ◽  
Author(s):  
Cristina Castells-Sala ◽  
Lourdes Recha-Sancho ◽  
Aida Llucià-Valldeperas ◽  
Carolina Soler-Botija ◽  
Antoni Bayes-Genis ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Progenitor Cells ◽  
Subcutaneous Adipose Tissue ◽  
Three Dimensional ◽  
Self Assembling ◽  
Subcutaneous Adipose

scholarly journals in vitrodevelopment of bioimplants made up of elastomeric scaffolds with peptide gel filling seeded with human subcutaneous adipose tissue-derived progenitor cells

2015 ◽  
Vol 103 (11) ◽  
pp. 3419-3430 ◽  
Author(s):  
Cristina Castells-Sala ◽  
Cristina Martínez-Ramos ◽  
Ana Vallés-Lluch ◽  
Manuel Monleón Pradas ◽  
Carlos Semino
Keyword(s):  
Adipose Tissue ◽  
Progenitor Cells ◽  
Subcutaneous Adipose Tissue ◽  
Subcutaneous Adipose

scholarly journals Melanocyte progenitor cells reside in human subcutaneous adipose tissue

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0256622
Author(s):  
Yuri Ikeda ◽  
Akino Wada ◽  
Toshio Hasegawa ◽  
Mutsumi Yokota ◽  
Masato Koike ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Progenitor Cells ◽  
Subcutaneous Adipose Tissue ◽  
Culture Medium ◽  
Skin Diseases ◽  
Subcutaneous Tissue ◽  
Basal Layer ◽  
Specific Culture ◽  
Ultraviolet Light B ◽  
Subcutaneous Adipose

Based on the assumption that some progenitor cells in an organ might reside in neighboring adipose tissue, we investigated whether melanocyte progenitor cells reside in human subcutaneous adipose tissue. First, we examined the expression of human melanoma black 45 (HMB45) and microphthalmia-associated transcription factor (MITF) in undifferentiated adipose-derived stem cells (ADSCs) by immunostaining, RT-PCR, and western blotting. These two markers were detected in undifferentiated ADSCs, and their expression levels were increased in differentiated ADSCs in melanocyte-specific culture medium. Other melanocytic markers (Melan A, MATP, Mel2, Mel EM, tyrosinase, KIT, and PAX3) were also detected at variable levels in undifferentiated ADSCs, and the expression of some markers was increased during differentiation into the melanocyte lineage. We further showed that ADSCs differentiated in melanocyte-specific culture medium localized in the basal layer and expressed tyrosinase and HMB45 in a 3D epidermal culture system. Melanin deposits were also induced by ultraviolet-light-B (UVB) irradiation. These results demonstrate that melanocyte progenitor cells reside in human subcutaneous adipose tissue and that these cells might have the potential to differentiate into mature melanocytes. Melanocyte and keratinocyte progenitors residing in human subcutaneous tissue can be used for the treatment of skin diseases and skin rejuvenation in the future.

scholarly journals Keratinocyte Progenitor Cells Reside in Human Subcutaneous Adipose Tissue

PLoS ONE ◽  
2015 ◽  
Vol 10 (2) ◽  
pp. e0118402 ◽  
Author(s):  
Toshio Hasegawa ◽  
Atsushi Sakamoto ◽  
Akino Wada ◽  
Tatsuo Fukai ◽  
Hideo Iida ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Progenitor Cells ◽  
Subcutaneous Adipose Tissue ◽  
Subcutaneous Adipose

Keratinocyte progenitor cells in human subcutaneous adipose tissue

2016 ◽  
Vol 84 (1) ◽  
pp. e54
Author(s):  
Hideo Iida ◽  
Toshio Hasegawa ◽  
Atsushi Sakamoto ◽  
Akino Wada ◽  
Tatsuo Fukai ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Progenitor Cells ◽  
Subcutaneous Adipose Tissue ◽  
Subcutaneous Adipose
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