Multicomponent Coculture System of Cancer Cells and Two Types of Stromal Cells for In Vitro Evaluation of Anticancer Drugs

2016 ◽  
Vol 22 (1) ◽  
pp. 20-29 ◽  
Author(s):  
Hironori Yamazoe ◽  
Yoshihisa Hagihara ◽  
Hisayuki Kobayashi
Keyword(s):  
Cancer Cells ◽  
Anticancer Drugs ◽  
Stromal Cells ◽  
In Vitro Evaluation ◽  
Coculture System

Cytotoxic Effect of the Combination of Gemcitabine and Atorvastatin Loaded in Microemulsion on the HCT116 Colon Cancer Cells

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Mayson H. Alkhatib ◽  
Dalal Al-Saedi ◽  
Wadiah S. Backer
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Anticancer Drugs ◽  
Therapeutic Potential ◽  
Morphological Changes ◽  
In Vitro Study ◽  
Colon Cancer Cells ◽  
Apoptosis Detection ◽  
Hct116 Cells

The combination of anticancer drugs in nanoparticles has great potential as a promising strategy to maximize efficacies by eradicating resistant, reduce the dosage of the drug and minimize toxicities on the normal cells. Gemcitabine (GEM), a nucleoside analogue, and atorvastatin (ATV), a cholesterol lowering agent, have shown anticancer effect with some limitations. The objective of this in vitro study was to evaluate the antitumor activity of the combination therapy of GEM and ATVencapsulated in a microemulsion (ME) formulation in the HCT116 colon cancer cells. The cytotoxicity and efficacy of the formulation were assessed by the 3- (4,5dimethylthiazole-2-yl)-2,5-diphyneltetrazolium bromide (MTT) assay. The mechanism of cell death was examined by observing the morphological changes of treated cells under light microscope, identifying apoptosis by using the ApopNexin apoptosis detection kit, and viewing the morphological changes in the chromatin structure stained with 4′,6-diamidino-2-phenylindole (DAPI) under the inverted fluorescence microscope. It has been found that reducing the concentration of GEM loaded on ME (GEM-ME) from 5μM to 1.67μM by combining it with 3.33μM of ATV in a ME formulation (GEM/2ATV-ME) has preserved the strong cytotoxicity of GEM-ME against HCT116 cells. The current study proved that formulating GEM with ATV in ME has improved the therapeutic potential of GEM and ATV as anticancer drugs.

SYSTEMATIC IN VITRO EVALUATION OF SURVIVIN DIRECTED ANTISENSE OLIGODEOXYNUCLEOTIDES IN BLADDER CANCER CELLS

2004 ◽  
Vol 171 (6 Part 1) ◽  
pp. 2471-2476 ◽  
Author(s):  
SUSANNE FUESSEL ◽  
BERND KUEPPERS ◽  
SHUANGLI NING ◽  
MATTHIAS KOTZSCH ◽  
KAI KRAEMER ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cancer Cells ◽  
Antisense Oligodeoxynucleotides ◽  
In Vitro Evaluation ◽  
Bladder Cancer Cells

scholarly journals Synthesis, Characterization and in Vitro Evaluation of Manganese Ferrite (MnFe2O4) Nanoparticles for Their Biocompatibility with Murine Breast Cancer Cells (4T1)

Molecules ◽  
2016 ◽  
Vol 21 (3) ◽  
pp. 312 ◽  
Author(s):  
Samikannu kanagesan ◽  
Sidek Aziz ◽  
Mansor Hashim ◽  
Ismayadi Ismail ◽  
Subramani Tamilselvan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Manganese Ferrite ◽  
In Vitro Evaluation ◽  
Mnfe2o4 Nanoparticles

In vitro evaluation of newly developed chalcone analogues in human cancer cells

2000 ◽  
Vol 46 (4) ◽  
pp. 305-312 ◽  
Author(s):  
Rosa De Vincenzo ◽  
Cristiano Ferlini ◽  
Mariagrazia Distefano ◽  
Cristiana Gaggini ◽  
Antonella Riva ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
In Vitro Evaluation ◽  
Human Cancer Cells

scholarly journals Design, synthesis and in vitro evaluation against human cancer cells of 5-methyl-5-styryl-2,5-dihydrofuran-2-ones, a new series of goniothalamin analogues

2013 ◽  
Vol 21 (17) ◽  
pp. 5107-5117 ◽  
Author(s):  
Marjorie Bruder ◽  
Débora Barbosa Vendramini-Costa ◽  
João Ernesto de Carvalho ◽  
Ronaldo Aloise Pilli
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
Design Synthesis ◽  
In Vitro Evaluation ◽  
Human Cancer Cells

In Vitro Evaluation of Rhodium and Osmium RAPTA Analogues:  The Case for Organometallic Anticancer Drugs Not Based on Ruthenium

2006 ◽  
Vol 25 (17) ◽  
pp. 4090-4096 ◽  
Author(s):  
Antoine Dorcier ◽  
Wee Han Ang ◽  
Sandra Bolaño ◽  
Luca Gonsalvi ◽  
Lucienne Juillerat-Jeannerat ◽  
...  
Keyword(s):  
Anticancer Drugs ◽  
In Vitro Evaluation
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