A Novel Method for Three-Dimensional Culture of Central Nervous System Neurons

2014 ◽  
Vol 20 (6) ◽  
pp. 485-492 ◽  
Author(s):  
Till B. Puschmann ◽  
Yolanda de Pablo ◽  
Carl Zandén ◽  
Johan Liu ◽  
Milos Pekny
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Three Dimensional ◽  
Novel Method ◽  
Three Dimensional Culture

Confocal laser microscopy of impregnated central nervous system tissue

1988 ◽  
Vol 46 ◽  
pp. 94-95
Author(s):  
J.N. Turner ◽  
M. Siemens ◽  
D. Szarowski ◽  
D.N. Collins
Keyword(s):  
Electron Microscopy ◽  
Central Nervous System ◽  
Nervous System ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Confocal Laser ◽  
High Contrast ◽  
Central Nervous System Tissue ◽  
Tissue Samples ◽  
Reflected Light

A classic preparation of central nervous system tissue (CNS) is the Golgi procedure popularized by Cajal. The method is partially specific as only a few cells are impregnated with silver chromate usualy after osmium post fixation. Samples are observable by light (LM) or electron microscopy (EM). However, the impregnation is often so dense that structures are masked in EM, and the osmium background may be undesirable in LM. Gold toning is used for a subtle but high contrast EM preparation, and osmium can be omitted for LM. We are investigating these preparations as part of a study to develop correlative LM and EM (particularly HVEM) methodologies in neurobiology. Confocal light microscopy is particularly useful as the impregnated cells have extensive three-dimensional structure in tissue samples from one to several hundred micrometers thick. Boyde has observed similar preparations in the tandem scanning reflected light microscope (TSRLM).

Three-dimensional sonographic features of fetal central nervous system anomaly

2000 ◽  
Vol 79 (8) ◽  
pp. 635-639 ◽  
Author(s):  
TOSHIYUKI HATA ◽  
TOSHIHIRO YANAGIHARA ◽  
MINAKO MATSUMOTO ◽  
UIKO HANAOKA ◽  
MARI UETA ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Three Dimensional ◽  
Central Nervous System Anomaly

A Novel Method of Labeling and Characterizing Migrating Neurons in the Developing Central Nervous System

2002 ◽  
Vol 174 (2) ◽  
pp. 259-265 ◽  
Author(s):  
Pavlos Alifragis ◽  
John G. Parnavelas ◽  
Bagirathy Nadarajah
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Novel Method
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