scholarly journals Natural Corneal Cell-Based Microenvironment as Prerequisite for Balanced 3D Corneal Epithelial Morphogenesis: A Promising Animal Experiment-Abandoning Tool in Ophthalmology

2014 ◽  
Vol 20 (4) ◽  
pp. 297-307 ◽  
Author(s):  
Simon Schulz ◽  
David Beck ◽  
Dougal Laird ◽  
Thorsten Steinberg ◽  
Pascal Tomakidi ◽  
...  
Keyword(s):  
Animal Experiment ◽  
Epithelial Morphogenesis ◽  
Corneal Epithelial ◽  
Corneal Cell

scholarly journals Cystic Fibrosis Transmembrane Conductance Regulator-Mediated Corneal Epithelial Cell Ingestion of Pseudomonas aeruginosaIs a Key Component in the Pathogenesis of Experimental Murine Keratitis

1999 ◽  
Vol 67 (3) ◽  
pp. 1481-1492 ◽  
Author(s):  
Tanweer S. Zaidi ◽  
Jeffrey Lyczak ◽  
Michael Preston ◽  
Gerald B. Pier
Keyword(s):  
Cystic Fibrosis ◽  
Epithelial Cells ◽  
Corneal Epithelial Cell ◽  
Transmembrane Conductance Regulator ◽  
Transmembrane Conductance ◽  
Corneal Epithelial Cells ◽  
Eye Infections ◽  
Corneal Epithelial ◽  
Corneal Cell ◽  
Cystic Fibrosis Transmembrane

ABSTRACT Previous findings indicate that the cystic fibrosis transmembrane conductance regulator (CFTR) is a ligand for Pseudomonas aeruginosa ingestion into respiratory epithelial cells. In experimental murine keratitis, P. aeruginosa enters corneal epithelial cells. We determined the importance of CFTR-mediated uptake of P. aeruginosa by corneal cells in experimental eye infections. Entry of noncytotoxic (exoU) P. aeruginosa into human and rabbit corneal cell cultures was inhibited with monoclonal antibodies and peptides specific to CFTR amino acids 108 to 117. Immunofluorescence microscopy and flow cytometry demonstrated CFTR in the intact murine corneal epithelium, and electron microscopy showed that CFTR binds to P. aeruginosa following corneal cell ingestion. In experimental murine eye infections, multiple additions of 5 nM CFTR peptide 103-117 to inocula of either cytotoxic (exoU +) or noncytotoxic P. aeruginosa resulted in large reductions in bacteria in the eye and markedly lessened eye pathology. Compared with wild-type C57BL/6 mice, heterozygous ΔF508 Cftr mice infected with P. aeruginosa had an approximately 10-fold reduction in bacterial levels in the eye and consequent reductions in eye pathology. Homozygous ΔF508 Cftr mice were nearly completely resistant to P. aeruginosa corneal infection. CFTR-mediated internalization of P. aeruginosa by buried corneal epithelial cells is critical to the pathogenesis of experimental eye infection, while in the lung, P. aeruginosa uptake by surface epithelial cells enhances P. aeruginosa clearance from this tissue.

scholarly journals Improvement In Rabbit Corneal Cell Suspension Viability After Freezing With Gingko Biloba Extrakt

2007 ◽  
Vol 50 (2) ◽  
pp. 145-148
Author(s):  
Murad Aktan ◽  
Berrin Okka ◽  
Mehmet Okka ◽  
Selcuk Duman
Keyword(s):  
Cell Viability ◽  
Antioxidant Capacity ◽  
Cell Suspension ◽  
Corneal Epithelium ◽  
Egb 761 ◽  
Single Cell Suspension ◽  
Corneal Epithelial ◽  
Corneal Cell ◽  
Gingko Biloba ◽  
The Media

We investigated whether the addition of Gingko Biloba extract (EGb 761) to rabbit corneal epithelial medium before cell freezing improved cell viability after freezing then thawing. After removal of corneas, they were treated with enzymes and the corneal epithelium was prepared as a single cell suspension in freezing media with or without EGb 761. After freezing for two weeks then thawing, a higher cell viability was found in the cornea cell suspensions which had been frozen pretreated with EGb 761 in the media. The improvement with corneal cell viability with EGb 761 pretreatment is postulated to be based on the antioxidant capacity of the plant extract.

scholarly journals Conditional deletion of Cited2 results in defective corneal epithelial morphogenesis and maintenance

2009 ◽  
Vol 334 (1) ◽  
pp. 243-252 ◽  
Author(s):  
Yu Chen ◽  
Eric C. Carlson ◽  
Zhi-Yi Chen ◽  
Anne Hamik ◽  
Mukesh K. Jain ◽  
...  
Keyword(s):  
Epithelial Morphogenesis ◽  
Corneal Epithelial ◽  
Conditional Deletion

Lysophosphatidic acid as a mediator for proinflammatory agonists in a human corneal epithelial cell line

2006 ◽  
Vol 291 (5) ◽  
pp. C1089-C1098 ◽  
Author(s):  
Zhihong Zhang ◽  
Zuguo Liu ◽  
Kathryn E. Meier
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Lysophosphatidic Acid ◽  
Corneal Epithelial Cell ◽  
Epithelial Cell Line ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cell ◽  
Corneal Cell ◽  
Tnf Α ◽  
Lpa Receptors

Lysophosphatidic acid (LPA) refers to a family of small phospholipid mediators that are generated in response to agonist stimulation in diverse cell types. LPA binds to G protein-coupled receptors to elicit numerous biological responses, including proliferation and inflammation. In this study, LPA production and response were characterized in a human corneal epithelial cell line, 2.040 pRSV-T. LPA levels in cells and medium are increased by exogenous 18:1 LPA (oleoyl-LPA), LPS, IL-1β, and TNF-α. LPS, IL-1β, and TNF-α, which mediate ocular inflammation, stimulate activation of p38, ERK, and Akt kinases in the corneal cell line. Similar responses are elicited by 18:1 LPA. Pertussis toxin (PTX) blocks LPA-induced activation of p38 and ERK but only slightly inhibits LPA-induced activation of Akt. All of the agonists tested, including LPA, stimulate proliferation of 2.040 pRSV-T cells. In these cells, both Akt and ERK pathways are important for LPA-induced proliferation. Thus PTX only partially suppresses the mitogenic response to LPA. Transcripts for the LPA receptors LPA1/EDG-2, LPA2/EDG-4, and LPA3/EDG-7 are expressed by the corneal cell line. Ki16425, an antagonist for LPA receptors, was used to explore the autocrine role of LPA. LPA-induced activations of p38, ERK, and Akt kinases, as well as proliferation, are inhibited by Ki16425. Ki16425 partially inhibits signal transduction and proliferation induced by the inflammatory agents tested. We conclude that LPA, produced in corneal epithelial cells in response to inflammatory agonists, contributes to mediating the mitogenic responses to these agonists in an autocrine fashion.

Urokinase Evaluated with the Experimental Radioactive Embolus

1967 ◽  
Vol 17 (03/04) ◽  
pp. 405-411
Author(s):  
M Hume
Keyword(s):  
Animal Experiment ◽  
Blood Clot ◽  
In Vitro Testing ◽  
Effective Agent

SummaryUrokinase and urokinase-activated plasmin have been given to the dog and rabbit. A thrombolytic state has been induced. Purified urokinase has induced lysis of the experimental radioactive blood clot embolus in the circulation. Demonstration of effectiveness in this animal experiment is hampered by inhibition of the agents in the circulation to a degree much greater than was noted in previous experiments with streptokinase. In vitro testing indicates that under proper conditions urokinase will be an effective agent in the treatment of human thromboembolism.

The effect of PRK on corneal epithelium thickness and pachmetry in treatment of myopia.

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Furkaan Majied Hamied ◽  
Deyaa Neama Kadhim ◽  
Sohaib A Mahmood
Keyword(s):  
Refractive Error ◽  
Anterior Segment ◽  
Thickness Change ◽  
Corneal Epithelial ◽  
Epithelial Thickness ◽  
Refractive Outcomes ◽  
Epithelium Thickness ◽  
Profile Changes ◽  
Corneal Epithelial Thickness ◽  
Myopic Astigmatism

In order to facilitate the corneal stromal ablation in photorefractive keratectomy the epithelium is removed so corneal repair associated with changes in epithelium and stroma. To study the corneal epithelial thickness and pachymetry profile changes after photorefractive keratec­tomy (PRK) for myopia. Retrospective analysis of the postoperative corneal epithelial thickness and pachymetry profile changes in 22 eyes of 12 patients treated with PRK for myopia or myopic astigmatism. Corneal and epithelial thickness maps within the central 6 mm were obtained by anterior segment spectral-domain optical coherence tomography (SD-OCT) preop­eratively and at 3 months postoperatively. Correlations between pachymetry,epithelial thickness changes and the amount of correction,were analyzed.Compared to preoperative values,the central 2 mm and the paracentral 2 to 5 mm zone epithelium was 1 ± 2.85 and 1 ± 3.11 μm thicker,respec­tively,at 3 months postoperatively (P <.05). The spheri­cal equivalent (SE) changed from-2.80 ± 2.028 diop­ters (D) preoperatively to -0.40 ± 0.42 D at 3 months postoperatively. Females show greater postoperative epithelial thickening, 2.6 ± 3.77 μm,than males,0.34 ± 1.98 μm. There was a trend toward greater epithelial thickening with a larger amount of programmed SE correction, and thinner preoperative epithelium. No correlation between epithelial thickness change and postoperative change in refraction was detected.Negative correlation between between age, refractive error,with the pre and post-operative pachymetry. In general female pachymetry reading is higher than it in male.The corneal epithelial thickness in­creased after PRK up to 3 months postoperatively. It was affected by the amount of myopia treated, gender, and preoperative epithelial thickness. The refractive outcomes did not affected by the postoperative epithelial thickening. Negative correlation between between age, refractive error, with the pre and post-operative pachymetry. In general female pachymetry reading is higher than it in male.

Managing Corneal Epithelial Defects Using High Molecular Weight Fluorescein to Prevent Contact Lens Staining and Removal of Bandage Contact Lenses

2020 ◽  
Vol 3 (8) ◽  
pp. 20-22
Author(s):  
Muhammad Hamza ◽  
Chrishan Gunasekera ◽  
Samar Nahas ◽  
Z CX Lin ◽  
Hatch Mukherjee ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Contact Lens ◽  
Contact Lenses ◽  
Corneal Epithelial
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