Human Skin Model Containing Melanocytes: Essential Role of Keratinocyte Growth Factor for Constitutive Pigmentation—Functional Response to α-Melanocyte Stimulating Hormone and Forskolin

2012 ◽  
Vol 18 (12) ◽  
pp. 947-957 ◽  
Author(s):  
Christine Duval ◽  
Corinne Chagnoleau ◽  
Florence Pouradier ◽  
Peggy Sextius ◽  
Elodie Condom ◽  
...  
Keyword(s):  
Growth Factor ◽  
Functional Response ◽  
Human Skin ◽  
Essential Role ◽  
Keratinocyte Growth Factor ◽  
Skin Model ◽  
Melanocyte Stimulating Hormone ◽  
Stimulating Hormone

scholarly journals Role of tyrosine kinase and protein kinase C in the steroidogenic actions of angiotensin II, α-melanocyte-stimulating hormone and corticotropin in the rat adrenal cortex

1995 ◽  
Vol 305 (2) ◽  
pp. 433-438 ◽  
Author(s):  
S Kapas ◽  
A Purbrick ◽  
J P Hinson
Keyword(s):  
Protein Kinase C ◽  
Angiotensin Ii ◽  
Protein Kinase ◽  
Tyrosine Kinase ◽  
Zona Glomerulosa ◽  
Aldosterone Secretion ◽  
Melanocyte Stimulating Hormone ◽  
Kinase C ◽  
Stimulating Hormone

The role of protein kinases in the steroidogenic actions of alpha-melanocyte-stimulating hormone (alpha-MSH), angiotensin II (AngII) and corticotropin (ACTH) in the rat adrenal zona glomerulosa was examined. Ro31-8220, a potent selective inhibitor of protein kinase C (PKC), inhibited both AngII- and alpha-MSH-stimulated aldosterone secretion but had no effect on aldosterone secretion in response to ACTH. The effect of Ro31-8220 on PKC activity was measured in subcellular fractions. Basal PKC activity was higher in cytosol than in membrane or nuclear fractions. Incubation of the zona glomerulosa with either alpha-MSH or AngII resulted in significant increases in PKC activity in the nuclear and cytosolic fractions and decreases in the membrane fraction. These effects were all inhibited by Ro31-8220. ACTH caused a significant increase in nuclear PKC activity only, and this was inhibited by Ro31-8220 without any significant effect on the steroidogenic response to ACTH, suggesting that PKC translocation in response to ACTH may be involved in another aspect of adrenal cellular function. Tyrosine phosphorylation has not previously been considered to be an important component of the response of adrenocortical cells to peptide hormones. Both AngII and alpha-MSH were found to activate tyrosine kinase, but ACTH had no effect, observations that have not been previously reported. Tyrphostin 23, a specific antagonist of tyrosine kinases, inhibited aldosterone secretion in response to AngII and alpha-MSH, but not ACTH. These data confirm the importance of PKC in the adrenocortical response to AngII and alpha-MSH, and, furthermore, indicate that tyrosine kinase may play a critical role in the steroidogenic actions of AngII and alpha-MSH in the rat adrenal zona glomerulosa.

Analogs of α-melanocyte stimulating hormone with high agonist potency and selectivity at human melanocortin receptor 1b: The role of Trp9 in molecular recognition

Biopolymers ◽  
2008 ◽  
Vol 89 (5) ◽  
pp. 401-408 ◽  
Author(s):  
Maria A. Bednarek ◽  
Tanya MacNeil ◽  
Rui Tang ◽  
Tung M. Fong ◽  
M. Angeles Cabello ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Melanocortin Receptor ◽  
Melanocyte Stimulating Hormone ◽  
Stimulating Hormone

scholarly journals Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling?

Vaccines ◽  
2018 ◽  
Vol 6 (3) ◽  
pp. 44 ◽  
Author(s):  
Maelíosa McCrudden ◽  
Katherine O’Donnell ◽  
Chris Irwin ◽  
Fionnuala Lundy
Keyword(s):  
Growth Factor ◽  
Keratinocyte Growth Factor ◽  
Statistical Significance ◽  
Host Defence ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Gingival Fibroblasts ◽  
Periodontal Tissues ◽  
Hepatocyte Growth ◽  
Periodontal Tissue Remodeling

Mounting evidence suggests that the host defence peptide, LL-37, plays a role in both inflammation and in wound healing; however, the role of this peptide in the remodeling and maintenance of oral tissues is not yet fully understood. Fibroblasts are the most abundant cell type within the periodontal tissues, and gingival fibroblasts play an important role in maintaining and repairing the gingival tissues which are constantly exposed to external insults. In this study we examined the direct effects of LL-37 treatment on gingival fibroblasts and found that LL-37 significantly increased secretion of both interleukin 8 (IL-8) and IL-6 from these cells. LL-37 tended to decrease matrix metalloproteinase (MMP) activity in gingival fibroblasts, but this decrease did not reach statistical significance. LL-37 significantly increased tissue inhibitor of metalloproteinase-1 (TIMP-1) production by gingival fibroblasts, but had no significant effect on TIMP-2 levels. LL-37 was also shown to significantly increase production of basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), and keratinocyte growth factor (KGF) in gingival fibroblasts. Taken together, these results suggest an important role for the host defence peptide, LL-37, in modulating the fibroblast response to remodeling in periodontal tissues.

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