scholarly journals Compartmental Hollow Fiber Capillary Membrane–Based Bioreactor Technology for In Vitro Studies on Red Blood Cell Lineage Direction of Hematopoietic Stem Cells

2012 ◽  
Vol 18 (2) ◽  
pp. 133-142 ◽  
Author(s):  
Greggory J. Housler ◽  
Toshio Miki ◽  
Eva Schmelzer ◽  
Christopher Pekor ◽  
Xiaokui Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Hematopoietic Stem Cells ◽  
Red Blood Cell ◽  
Hollow Fiber ◽  
Cell Lineage ◽  
In Vitro Studies ◽  
Hematopoietic Stem ◽  
Capillary Membrane

scholarly journals Understanding the Journey of Human Hematopoietic Stem Cell Development

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Akhilesh Kumar ◽  
Saritha S. D’Souza ◽  
Abir S. Thakur
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Hematopoietic Stem Cells ◽  
Human Blood ◽  
Molecular Mechanisms ◽  
Inflammatory Responses ◽  
Current Knowledge ◽  
Hematopoietic Stem ◽  
Hematopoietic Development

Hematopoietic stem cells (HSCs) surface during embryogenesis leading to the genesis of the hematopoietic system, which is vital for immune function, homeostasis balance, and inflammatory responses in the human body. Hematopoiesis is the process of blood cell formation, which initiates from hematopoietic stem/progenitor cells (HSPCs) and is responsible for the generation of all adult blood cells. With their self-renewing and pluripotent properties, human pluripotent stem cells (hPSCs) provide an unprecedented opportunity to createin vitromodels of differentiation that will revolutionize our understanding of human development, especially of the human blood system. The utilization of hPSCs provides newfound approaches for studying the origins of human blood cell diseases and generating progenitor populations for cell-based treatments. Current shortages in our knowledge of adult HSCs and the molecular mechanisms that control hematopoietic development in physiological and pathological conditions can be resolved with better understanding of the regulatory networks involved in hematopoiesis, their impact on gene expression, and further enhance our ability to develop novel strategies of clinical importance. In this review, we delve into the recent advances in the understanding of the various cellular and molecular pathways that lead to blood development from hPSCs and examine the current knowledge of human hematopoietic development. We also review howin vitrodifferentiation of hPSCs can undergo hematopoietic transition and specification, including major subtypes, and consider techniques and protocols that facilitate the generation of hematopoietic stem cells.

Strategies to Protect Hematopoietic Stem Cells from Culture-Induced Stress Conditions

2020 ◽  
Vol 15 ◽  
Author(s):  
Fatima Aerts-Kaya
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Ex Vivo ◽  
Stress Conditions ◽  
Stress Factors ◽  
Hematopoietic Stem ◽  
Induced Stress

: In contrast to their almost unlimited potential for expansion in vivo and despite years of dedicated research and optimization of expansion protocols, the expansion of Hematopoietic Stem Cells (HSCs) in vitro remains remarkably limited. Increased understanding of the mechanisms that are involved in maintenance, expansion and differentiation of HSCs will enable the development of better protocols for expansion of HSCs. This will allow procurement of HSCs with long-term engraftment potential and a better understanding of the effects of the external influences in and on the hematopoietic niche that may affect HSC function. During collection and culture of HSCs, the cells are exposed to suboptimal conditions that may induce different levels of stress and ultimately affect their self-renewal, differentiation and long-term engraftment potential. Some of these stress factors include normoxia, oxidative stress, extra-physiologic oxygen shock/stress (EPHOSS), endoplasmic reticulum (ER) stress, replicative stress, and stress related to DNA damage. Coping with these stress factors may help reduce the negative effects of cell culture on HSC potential, provide a better understanding of the true impact of certain treatments in the absence of confounding stress factors. This may facilitate the development of better ex vivo expansion protocols of HSCs with long-term engraftment potential without induction of stem cell exhaustion by cellular senescence or loss of cell viability. This review summarizes some of available strategies that may be used to protect HSCs from culture-induced stress conditions.

EXPANSION OF HEMATOPOIETIC STEM CELLS IN VITRO AS A MODEL SYSTEM FOR HUMAN TISSUE ENGINEERING

2000 ◽  
Vol 31 (3) ◽  
pp. 499-509 ◽  
Author(s):  
Joel S. Greenberger ◽  
Julie P. Goff ◽  
Jason Bush ◽  
Alfred Bahnson ◽  
Douglas Koebler ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Human Tissue ◽  
Model System ◽  
Hematopoietic Stem

3040 – IN VITRO RECAPITULATION OF MURINE T CELL DEVELOPMENT FROM SINGLE HEMATOPOIETIC STEM CELLS

2020 ◽  
Vol 88 ◽  
pp. S51
Author(s):  
Victoria Sun ◽  
Amelie Montel-Hagen ◽  
David Casero ◽  
Steven Tsai ◽  
Alexandre Zampieri ◽  
...  
Keyword(s):  
Stem Cells ◽  
T Cell ◽  
Hematopoietic Stem Cells ◽  
T Cell Development ◽  
Cell Development ◽  
Hematopoietic Stem
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