Hepatic Differentiation of Human Embryonic Stem Cells Is Promoted by Three-Dimensional Dynamic Perfusion Culture Conditions

2011 ◽  
Vol 17 (5) ◽  
pp. 557-568 ◽  
Author(s):  
Toshio Miki ◽  
Alexander Ring ◽  
Jörg Gerlach
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Perfusion Culture ◽  
Culture Conditions ◽  
Hepatic Differentiation

scholarly journals Hepatic Differentiation and Maturation of Human Embryonic Stem Cells Cultured in a Perfused Three-Dimensional Bioreactor

2013 ◽  
Vol 22 (4) ◽  
pp. 581-594 ◽  
Author(s):  
Louise Sivertsson ◽  
Jane Synnergren ◽  
Janne Jensen ◽  
Petter Björquist ◽  
Magnus Ingelman-Sundberg
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Hepatic Differentiation ◽  
Cells Cultured

scholarly journals Transcriptomic analysis of naïve human embryonic stem cells cultured in three-dimensional PEG scaffolds

2020 ◽  
Author(s):  
Christina McKee ◽  
Christina Brown ◽  
Shreeya Bakshi ◽  
Keegan Walker ◽  
Chhabi K. Govind ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Expression Profiles ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Culture Conditions ◽  
Molecular Characteristics ◽  
Regulation Of Transcription

AbstractDerivation of primed and naïve human embryonic stem cells (ESCs) have prompted an increased interest in devising culture conditions for maintaining their pluripotency and differential potential. Naïve ESCs are characterized by improved viability, proliferation, and differentiation capacity in comparison to primed ESCs. However, traditional two-dimensional (2-D) cell culture techniques fail to mimic the three-dimensional (3-D) in vivo microenvironment, which results in altered morphological and molecular characteristics of ESCs. Here, we describe the use of 3-D self-assembling scaffolds that support growth and maintenance of the naïve state characteristics of human ESC line, Elf1. Scaffolds were formed via a Michael addition reaction upon combination of two 8-arm polyethylene glycol (PEG) polymers functionalized with thiol (PEG-8-SH) and acrylate (PEG-8-Acr) end groups. 3-D scaffolds not only maintained the naïve state, but also supported long-term growth for up to 3 weeks without requiring routine passaging and manipulation. 3-D grown cells exhibited upregulation of core (OCT4, NANOG, and SOX2) and naïve (KLF17, KLF4, TFCP2L1, DPPA3, and DNMT3L) genes. These genes returned to normal levels when 3-D grown cells were propagated under 2-D culture conditions. Examination of RNA-sequencing demonstrated significant changes in gene expression profiles between 2-D and 3-D grown Elf1 cells. Gene Ontology analysis revealed upregulation of biological processes involved in the regulation of transcription and translation, as well as β-catenin-TCF complex assembly, extracellular matrix organization, and chromatin remodeling in 3-D grown Elf1 cells. 3-D culture conditions also induced upregulation of genes associated with several signaling pathways including Wnt signaling and focal adhesion. However, p53 signaling pathway associated genes were downregulated under these culture conditions. Our findings provide insight into the possible mechanisms of prolonged self-renewal as well as upregulation of pluripotent genes stimulated by the transduction of mechanical signals from the 3-D microenvironment.

scholarly journals Teratoma formation of human embryonic stem cells in three-dimensional perfusion culture bioreactors

2012 ◽  
Vol 7 (9) ◽  
pp. 729-741 ◽  
Author(s):  
H. Stachelscheid ◽  
A. Wulf-Goldenberg ◽  
K. Eckert ◽  
J. Jensen ◽  
J. Edsbagge ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Perfusion Culture ◽  
Teratoma Formation

Substrate-mediated commitment of human embryonic stem cells for hepatic differentiation

2016 ◽  
Vol 104 (11) ◽  
pp. 2861-2872 ◽  
Author(s):  
Mohammad Kazemi Ashtiani ◽  
Mojgan Zandi ◽  
Jalal Barzin ◽  
Yaser Tahamtani ◽  
Mohammad Hossein Ghanian ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Hepatic Differentiation

GRGD‐decorated three‐dimensional nanoworm hydrogels for culturing human embryonic stem cells

2019 ◽  
Vol 57 (18) ◽  
pp. 1956-1963 ◽  
Author(s):  
Stefanie Kessel ◽  
Nilay Thakar ◽  
Zhongfan Jia ◽  
Ernst J. Wolvetang ◽  
Michael J. Monteiro
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Three Dimensional ◽  
Embryonic Stem

scholarly journals Three-Dimensional Otic Neuronal Progenitor Spheroids Derived from Human Embryonic Stem Cells

2020 ◽  
Author(s):  
Rachel A. Heuer ◽  
Kevin T. Nella ◽  
Hsiang-Tsun Chang ◽  
Kyle S. Coots ◽  
Andrew M. Oleksijew ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Neuronal Progenitor

Methods for Expansion of Three-Dimensional Cultures of Human Embryonic Stem Cells Using a Thermoresponsive Polymer

2018 ◽  
Vol 24 (3) ◽  
pp. 146-157 ◽  
Author(s):  
Xiaoli Chen ◽  
Linda Harkness ◽  
Zhongfan Jia ◽  
Andrew Prowse ◽  
Michael J. Monteiro ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Thermoresponsive Polymer
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