Tissue Engineering of Cementum/Periodontal-Ligament Complex Using a Novel Three-Dimensional Pellet Cultivation System for Human Periodontal Ligament Stem Cells

2009 ◽  
Vol 15 (4) ◽  
pp. 571-581 ◽  
Author(s):  
Zhenhua Yang ◽  
Fang Jin ◽  
Xiaojun Zhang ◽  
Dandan Ma ◽  
Chun Han ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Periodontal Ligament ◽  
Three Dimensional ◽  
Periodontal Ligament Stem Cells ◽  
Cultivation System

scholarly journals Zein/gelatin/nanohydroxyapatite nanofibrous scaffolds are biocompatible and promote osteogenic differentiation of human periodontal ligament stem cells

2019 ◽  
Vol 7 (5) ◽  
pp. 1973-1983 ◽  
Author(s):  
Qianmin Ou ◽  
Yingling Miao ◽  
Fanqiao Yang ◽  
Xuefeng Lin ◽  
Li-Ming Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Periodontal Ligament ◽  
Periodontal Ligament Stem Cells ◽  
Nanofibrous Scaffolds

In bone tissue engineering, it is important for biomaterials to promote the osteogenic differentiation of stem cells to achieve tissue regeneration.

scholarly journals MicroRNA-214 Suppresses Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting ATF4

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Siqi Yao ◽  
Wei Zhao ◽  
Qianmin Ou ◽  
Lanchen Liang ◽  
Xuefeng Lin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Periodontal Ligament ◽  
Target Prediction ◽  
Cell Types ◽  
Luciferase Reporter ◽  
Periodontal Ligament Stem Cells ◽  
Activating Transcription Factor 4 ◽  
Activating Transcription Factor

Periodontitis is the main cause of adult tooth loss. Stem cell-based tissue engineering has become a promising therapy for periodontitis treatment. To date, human periodontal ligament stem cells (hPDLSCs) have been shown to be a favorable source for tissue engineering, but modulatory mechanisms of hPDLSCs remain unclear. Approximately 60% of mammalian genes are the targets of over 2000 miRNAs in multiple human cell types, and miRNAs are able to influence various biological processes in the human body, including bone formation. In this study, we found that after osteogenic induction, miR-214 was significantly decreased in hPDLSCs; therefore, we examined the effects of miR-214 on osteogenic differentiation. Computational miRNA target prediction analyses and luciferase reporter assays revealed that activating transcription factor 4 (ATF4) is a direct target of miR-214. We prepared cells overexpressing miR-214 and found that miR-214 negatively regulates osteogenic differentiation of hPDLSCs. For the target of miR-214, ATF4 protein expression level was decreased after induction. In conclusion, we found that miR-214-ATF4 axis is a novel pathway for regulating hPDLSC osteogenic differentiation.

Application of eGFP to label human periodontal ligament stem cells in periodontal tissue engineering

2012 ◽  
Vol 57 (9) ◽  
pp. 1241-1250 ◽  
Author(s):  
Yong Wen ◽  
Jing Lan ◽  
Haiyun Huang ◽  
Meijiao Yu ◽  
Jun Cui ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Periodontal Ligament ◽  
Periodontal Tissue ◽  
Periodontal Ligament Stem Cells

scholarly journals An antibacterial and injectable calcium phosphate scaffold delivering human periodontal ligament stem cells for bone tissue engineering

RSC Advances ◽  
2020 ◽  
Vol 10 (66) ◽  
pp. 40157-40170
Author(s):  
Hong Chen ◽  
Hui Yang ◽  
Michael D. Weir ◽  
Abraham Schneider ◽  
Ke Ren ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Periodontal Ligament ◽  
Periodontal Ligament Stem Cells

Osteomyelitis and post-operative infections are major problems in orthopedic, dental and craniofacial surgeries.

scholarly journals Human periodontal ligament stem cells on calcium phosphate scaffold delivering platelet lysate to enhance bone regeneration

RSC Advances ◽  
2019 ◽  
Vol 9 (70) ◽  
pp. 41161-41172 ◽  
Author(s):  
Zeqing Zhao ◽  
Jin Liu ◽  
Michael D. Weir ◽  
Ning Zhang ◽  
Li Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Calcium Phosphate ◽  
Bone Regeneration ◽  
Periodontal Ligament ◽  
Platelet Lysate ◽  
Engineering Applications ◽  
Periodontal Ligament Stem Cells

Human periodontal ligament stem cells (hPDLSCs) are promising for tissue engineering applications but have received relatively little attention.

scholarly journals Effects of Intermittent Administration of Parathyroid Hormone (1-34) on Bone Differentiation in Stromal Precursor Antigen-1 Positive Human Periodontal Ligament Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Xiaoxiao Wang ◽  
Yanlan Wang ◽  
Xubin Dai ◽  
Tianyu Chen ◽  
Fanqiao Yang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Parathyroid Hormone ◽  
Periodontal Ligament ◽  
Bone Destruction ◽  
Engineering Applications ◽  
Periodontal Ligament Stem Cells ◽  
Bone Differentiation ◽  
Potential Applications ◽  
Intermittent Pth

Periodontitis is the most common cause of tooth loss and bone destruction in adults worldwide. Human periodontal ligament stem cells (hPDLSCs) may represent promising new therapeutic biomaterials for tissue engineering applications. Stromal precursor antigen-1 (STRO-1) has been shown to have roles in adherence, proliferation, and multipotency. Parathyroid hormone (PTH) has been shown to enhance proliferation in osteoblasts. Therefore, in this study, we aimed to compare the functions of STRO-1(+) and STRO-1(−) hPDLSCs and to investigate the effects of PTH on the osteogenic capacity of STRO-1(+) hPDLSCs in order to evaluate their potential applications in the treatment of periodontitis. Our data showed that STRO-1(+) hPDLSCs expressed higher levels of the PTH-1 receptor (PTH1R) than STRO-1(−) hPDLSCs. In addition, intermittent PTH treatment enhanced the expression of PTH1R and osteogenesis-related genes in STRO-1(+) hPDLSCs. PTH-treated cells also exhibited increased alkaline phosphatase activity and mineralization ability. Therefore, STRO-1(+) hPDLSCs represented a more promising cell resource for biomaterials and tissue engineering applications. Intermittent PTH treatment improved the capacity for STRO-1(+) hPDLSCs to repair damaged tissue and ameliorate the symptoms of periodontitis.

scholarly journals In Vitro Characterization of Periodontal Ligament Stem Cells Derived from Supernumerary Teeth in Three-Dimensional Culture Method

2021 ◽  
Vol 11 (13) ◽  
pp. 6040
Author(s):  
Yun Yeong Jeong ◽  
Mi Sun Kim ◽  
Ko Eun Lee ◽  
Ok Hyung Nam ◽  
Ji-Hyun Jang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Periodontal Ligament ◽  
Three Dimensional ◽  
3D Culture ◽  
Culture Method ◽  
Supernumerary Teeth ◽  
Periodontal Ligament Stem Cells

Objective: The aim of this study was to compare the characteristics of periodontal ligament stem cells derived from supernumerary teeth (sPDLSCs), cultured using a three-dimensional (3D) method and a conventional two-dimensional (2D) method. Methods: The morphology, viability, and osteogenic differentiation of the cells were analyzed. In addition, gene expression was analyzed by RNA sequencing, to characterize the functional differences. Results: The diameter of the 3D-cultured sPDLSCs decreased over time, but the spheroid shape was maintained for 7 days. The osteogenic differentiation was similar in the 2D and 3D. The gene expression related to the extracellular matrix (7.3%), angiogenesis (5.6%), cell proliferation (4.6%), inflammatory response (3.7%), and cell migration (3.5%) differed (p < 0.05). Conclusions: Within the limitations of this study, sPDLSCs varied in formation and function, depending on the culture method. In future, it is necessary to study tissue engineering using the advantages of 3D culture and the fewer ethical problems of supernumerary teeth.

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