The Stiffness and Structure of Three-Dimensional Printed Hydrogels Direct the Differentiation of Mesenchymal Stromal Cells Toward Adipogenic and Osteogenic Lineages

2015 ◽  
Vol 21 (3-4) ◽  
pp. 740-756 ◽  
Author(s):  
Daniela F. Duarte Campos ◽  
Andreas Blaeser ◽  
Anne Korsten ◽  
Sabine Neuss ◽  
Jörg Jäkel ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Three Dimensional

scholarly journals Three-dimensional cartilage-derived construct derived from multipotent mesenchymal stromal cells

2020 ◽  
Vol 22 ◽  
pp. 02014
Author(s):  
Oleg Makeyev ◽  
Arteom Korotkov ◽  
Svetlana Kostyukova ◽  
Daria Sichkar ◽  
Elizaveta Yakovleva
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Three Dimensional ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Cartilage Tissue ◽  
Cartilage Surface ◽  
Initial Stage ◽  
Conventional Drug ◽  
Surgical Methods ◽  
Further Development

Damage to the cartilage surface of the joints is a common pathology. Conventional drug therapies are effective only at the initial stage of the disease and only slow down the further development of the disease. In subsequent stages, it is necessary to apply surgical methods from replacing a defect to complete prosthetics of a damaged joint. In order to replace defects in cartilage tissue, a three-dimensional bioequivalent was created based on autologous multipotent mesenchymal stromal cells.

scholarly journals Chondrogenic differentiation of human bone marrow‐derived mesenchymal stromal cells in a three‐dimensional environment

2019 ◽  
Vol 235 (4) ◽  
pp. 3497-3507
Author(s):  
Eve Salonius ◽  
Leena Kontturi ◽  
Anita Laitinen ◽  
Anne‐Marie Haaparanta ◽  
Matti Korhonen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Chondrogenic Differentiation ◽  
Three Dimensional ◽  
Human Bone ◽  
Human Bone Marrow

scholarly journals Hematopoietic Stem and Progenitor Cell Expansion in Contact with Mesenchymal Stromal Cells in a Hanging Drop Model Uncovers Disadvantages of 3D Culture

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Olga Schmal ◽  
Jan Seifert ◽  
Tilman E. Schäffer ◽  
Christina B. Walter ◽  
Wilhelm K. Aicher ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Ex Vivo ◽  
Three Dimensional ◽  
3D Culture ◽  
Hanging Drop ◽  
Mixed Cell ◽  
Hematopoietic Stem ◽  
Extracellular Matrix Components ◽  
Stem And Progenitor Cells

Efficientex vivoexpansion of hematopoietic stem cells with a concomitant preservation of stemness and self-renewal potential is still an unresolved ambition. Increased numbers of methods approaching this issue using three-dimensional (3D) cultures were reported. Here, we describe a simplified 3D hanging drop model for the coculture of cord blood-derived CD34+hematopoietic stem and progenitor cells (HSPCs) with bone marrow-derived mesenchymal stromal cells (MSCs). When seeded as a mixed cell suspension, MSCs segregated into tight spheroids. Despite the high expression of niche-specific extracellular matrix components by spheroid-forming MSCs, HSPCs did not migrate into the spheroids in the initial phase of coculture, indicating strong homotypic interactions of MSCs. After one week, however, HSPC attachment increased considerably, leading to spheroid collapse as demonstrated by electron microscopy and immunofluorescence staining. In terms of HSPC proliferation, the conventional 2D coculture system was superior to the hanging drop model. Furthermore, expansion of primitive hematopoietic progenitors was more favored in 2D than in 3D, as analyzed in colony-forming assays. Conclusively, our data demonstrate that MSCs, when arranged with a spread (monolayer) shape, exhibit better HSPC supportive qualities than spheroid-forming MSCs. Therefore, 3D systems are not necessarily superior to traditional 2D culture in this regard.

Enhanced differentiation potential of human amniotic mesenchymal stromal cells by using three-dimensional culturing

2013 ◽  
Vol 352 (3) ◽  
pp. 523-535 ◽  
Author(s):  
Xue Lin ◽  
Hao Yu Li ◽  
Lian Feng Chen ◽  
Bo Jiang Liu ◽  
Yian Yao ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Three Dimensional ◽  
Differentiation Potential
Sign in / Sign up

Export Citation Format

Share Document