Collagen Type XII and Versican Are Present in the Early Stages of Cartilage Tissue Formation by Both Redifferentating Passaged and Primary Chondrocytes

2015 ◽  
Vol 21 (3-4) ◽  
pp. 683-693 ◽  
Author(s):  
Drew W. Taylor ◽  
Nazish Ahmed ◽  
Justin Parreno ◽  
Gregory P. Lunstrum ◽  
Allan E. Gross ◽  
...  
Keyword(s):  
Collagen Type ◽  
Cartilage Tissue ◽  
Tissue Formation ◽  
Primary Chondrocytes ◽  
Early Stages

Primary chondrocytes enhance cartilage tissue formation upon co-culture with a range of cell types

Soft Matter ◽  
2010 ◽  
Vol 6 (20) ◽  
pp. 5080 ◽  
Author(s):  
Jeanine A. A. Hendriks ◽  
Razvan L. Miclea ◽  
Roka Schotel ◽  
Ewart de Bruijn ◽  
Lorenzo Moroni ◽  
...  
Keyword(s):  
Cell Types ◽  
Cartilage Tissue ◽  
Tissue Formation ◽  
Primary Chondrocytes

Electromagnetic fields enhance chondrogenesis of human adipose-derived stem cells in a chondrogenic microenvironment in vitro

2013 ◽  
Vol 114 (5) ◽  
pp. 647-655 ◽  
Author(s):  
Chung-Hwan Chen ◽  
Yi-Shan Lin ◽  
Yin-Chih Fu ◽  
Chih-Kuang Wang ◽  
Shun-Cheng Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Electromagnetic Field ◽  
Cell Viability ◽  
Chondrogenic Differentiation ◽  
Collagen Type ◽  
Cartilage Tissue ◽  
Pulse Electromagnetic Field ◽  
Type I ◽  
Adipose Derived Stem Cells ◽  
Pemf Treatment

We tested the hypothesis that electromagnetic field (EMF) stimulation enhances chondrogenesis in human adipose-derived stem cells (ADSCs) in a chondrogenic microenvironment. A two-dimensional hyaluronan (HA)-coated well (2D-HA) and a three-dimensional pellet culture system (3D-pellet) were used as chondrogenic microenvironments. The ADSCs were cultured in 2D-HA or 3D-pellet, and then treated with clinical-use pulse electromagnetic field (PEMF) or the innovative single-pulse electromagnetic field (SPEMF) stimulation. The cytotoxicity, cell viability, and chondrogenic and osteogenic differentiations were analyzed after PEMF or SPEMF treatment. The modules of PEMF and SPEMF stimulations used in this study did not cause cytotoxicity or alter cell viability in ADSCs. Both PEMF and SPEMF enhanced the chondrogenic gene expression (SOX-9, collagen type II, and aggrecan) of ADSCs cultured in 2D-HA and 3D-pellet. The expressions of bone matrix genes (osteocalcin and collagen type I) of ADSCs were not changed after SPEMF treatment in 2D-HA and 3D-pellet; however, they were enhanced by PEMF treatment. Both PEMF and SPEMF increased the cartilaginous matrix (sulfated glycosaminoglycan) deposition of ADSCs. However, PEMF treatment also increased mineralization of ADSCs, but SPEMF treatment did not. Both PEMF and SPEMF enhanced chondrogenic differentiation of ADSCs cultured in a chondrogenic microenvironment. SPEMF treatment enhanced ADSC chondrogenesis, but not osteogenesis, when the cells were cultured in a chondrogenic microenvironment. However, PEMF enhanced both osteogenesis and chondrogenesis under the same conditions. Thus the combination of a chondrogenic microenvironment with SPEMF stimulation can promote chondrogenic differentiation of ADSCs and may be applicable to articular cartilage tissue engineering.

scholarly journals Cartilage tissue formation from human adipose-derived stem cells via herbal component (Avocado/soybean unsaponifiables) in scaffold-free culture system

2020 ◽  
Vol 17 (1) ◽  
pp. 54
Author(s):  
Nazem Ghasemi ◽  
Arefeh Basiri ◽  
Batool Hashemibeni ◽  
Mohammad Kazemi ◽  
Ali Valiani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Culture System ◽  
Cartilage Tissue ◽  
Adipose Derived Stem Cells ◽  
Tissue Formation

Cartilage tissue formation through assembly of microgels containing mesenchymal stem cells

2018 ◽  
Vol 77 ◽  
pp. 48-62 ◽  
Author(s):  
Fanyi Li ◽  
Vinh X. Truong ◽  
Philipp Fisch ◽  
Clara Levinson ◽  
Veronica Glattauer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cartilage Tissue ◽  
Tissue Formation

Early Stages of Crystal Nucleation in Hard Tissue Formation

1981 ◽  
pp. 974-982 ◽  
Author(s):  
H. J. Höhling ◽  
J. Althoff ◽  
R. H. Barckhaus ◽  
E.-R. Krefting ◽  
G. Lissner ◽  
...  
Keyword(s):  
Crystal Nucleation ◽  
Tissue Formation ◽  
Hard Tissue ◽  
Early Stages

scholarly journals In Vitro Fabrication of Hybrid Bone/Cartilage Complex Using Mouse Induced Pluripotent Stem Cells

2020 ◽  
Vol 21 (2) ◽  
pp. 581 ◽  
Author(s):  
Phoonsuk Limraksasin ◽  
Takeru Kondo ◽  
Maolin Zhang ◽  
Hiroko Okawa ◽  
Thanaphum Osathanon ◽  
...  
Keyword(s):  
Cartilage Tissue ◽  
Marker Genes ◽  
Induction Medium ◽  
Mechanical Stimuli ◽  
Tissue Formation ◽  
Osteogenic Induction Medium ◽  
Osteogenic Induction ◽  
Chondrogenic Marker ◽  
Induced Pluripotent

Cell condensation and mechanical stimuli play roles in osteogenesis and chondrogenesis; thus, they are promising for facilitating self-organizing bone/cartilage tissue formation in vitro from induced pluripotent stem cells (iPSCs). Here, single mouse iPSCs were first seeded in micro-space culture plates to form 3-dimensional spheres. At day 12, iPSC spheres were subjected to shaking culture and maintained in osteogenic induction medium for 31 days (Os induction). In another condition, the osteogenic induction medium was replaced by chondrogenic induction medium at day 22 and maintained for a further 21 days (Os-Chon induction). Os induction produced robust mineralization and some cartilage-like tissue, which promoted expression of osteogenic and chondrogenic marker genes. In contrast, Os-Chon induction resulted in partial mineralization and a large area of cartilage tissue, with greatly increased expression of chondrogenic marker genes along with osterix and collagen 1a1. Os-Chon induction enhanced mesodermal lineage commitment with brachyury expression followed by high expression of lateral plate and paraxial mesoderm marker genes. These results suggest that combined use of micro-space culture and mechanical stimuli facilitates hybrid bone/cartilage tissue formation from iPSCs, and that the bone/cartilage tissue ratio in iPSC constructs could be manipulated through the induction protocol.

scholarly journals Biophysical Stimuli: A Review of Electrical and Mechanical Stimulation in Hyaline Cartilage

Cartilage ◽  
2017 ◽  
Vol 10 (2) ◽  
pp. 157-172 ◽  
Author(s):  
Juan J. Vaca-González ◽  
Johana M. Guevara ◽  
Miguel A. Moncayo ◽  
Hector Castro-Abril ◽  
Yoshie Hata ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Proliferation ◽  
Mechanical Stimulation ◽  
Collagen Type ◽  
Hyaline Cartilage ◽  
Cartilage Tissue ◽  
Mechanical Stimuli ◽  
The Past ◽  
Cartilage Extracellular Matrix ◽  
Biomechanical Changes

Objective Hyaline cartilage degenerative pathologies induce morphologic and biomechanical changes resulting in cartilage tissue damage. In pursuit of therapeutic options, electrical and mechanical stimulation have been proposed for improving tissue engineering approaches for cartilage repair. The purpose of this review was to highlight the effect of electrical stimulation and mechanical stimuli in chondrocyte behavior. Design Different information sources and the MEDLINE database were systematically revised to summarize the different contributions for the past 40 years. Results It has been shown that electric stimulation may increase cell proliferation and stimulate the synthesis of molecules associated with the extracellular matrix of the articular cartilage, such as collagen type II, aggrecan and glycosaminoglycans, while mechanical loads trigger anabolic and catabolic responses in chondrocytes. Conclusion The biophysical stimuli can increase cell proliferation and stimulate molecules associated with hyaline cartilage extracellular matrix maintenance.

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