scholarly journals In Vitro Development and Characterization of a Tissue-Engineered Conduit Resembling Esophageal Wall Using Human and Pig Skeletal Myoblast, Oral Epithelial Cells, and Biologic Scaffolds

2013 ◽  
Vol 19 (19-20) ◽  
pp. 2242-2252 ◽  
Author(s):  
Tigran Poghosyan ◽  
Sebastien Gaujoux ◽  
Valerie Vanneaux ◽  
Patrick Bruneval ◽  
Thomas Domet ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Esophageal Wall ◽  
Skeletal Myoblast ◽  
In Vitro Development ◽  
Oral Epithelial Cells ◽  
Vitro Development ◽  
Oral Epithelial

scholarly journals Immortalization and characterization of normal oral epithelial cells without using HPV and SV40 genes

2011 ◽  
Vol 8 (1) ◽  
pp. 20-28 ◽  
Author(s):  
Toshiro Kibe ◽  
Michiko Kishida ◽  
Masayuki Kamino ◽  
Mikio Iijima ◽  
Lin Chen ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Oral Epithelial Cells ◽  
Oral Epithelial

scholarly journals In Vitro Studies on Erythrosine-Based Photodynamic Therapy of Malignant and Pre-Malignant Oral Epithelial Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e34475 ◽  
Author(s):  
Abhishek D. Garg ◽  
Muthiah Bose ◽  
Mohammed I. Ahmed ◽  
William A. Bonass ◽  
Simon R. Wood
Keyword(s):  
Photodynamic Therapy ◽  
Epithelial Cells ◽  
In Vitro Studies ◽  
Oral Epithelial Cells ◽  
Oral Epithelial

scholarly journals The Influence of Oral Bacteria on Epithelial Cell MigrationIn Vitro

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Alexa M. G. A. Laheij ◽  
Johannes J. de Soet ◽  
Enno C. I. Veerman ◽  
Jan G. M. Bolscher ◽  
Cor van Loveren
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Oral Bacteria ◽  
Bacterial Cells ◽  
Oral Epithelial Cells ◽  
Prevotella Nigrescens ◽  
Hematopoetic Stem Cell ◽  
Oral Epithelial ◽  
Hematopoetic Stem Cell Transplantation

Oral ulcerations often arise as a side effect from chemo- and radiation therapy. In a previous clinical study,Porphyromonas gingivaliswas identified as a positive predictor for oral ulcerations after hematopoetic stem cell transplantation, possibly incriminatingP. gingivalisin delayed healing of the ulcerations. Therefore, it was tested whetherP. gingivalisand its secreted products could inhibit the migration of oral epithelial cells in anin vitroscratch assay. To compare, the oral bacteriaPrevotella nigrescens,Prevotella intermedia,Tannerella forsythia, andStreptococcus mitiswere included. A standardized scratch was made in a confluent layer of human oral epithelial cells. The epithelial cells were challenged with bacterial cells and with medium containing secretions of these bacteria. Closure of the scratch was measured after 17 h using a phase contrast microscope.P. gingivalis,P. nigrescens, and secretions ofP. gingivalisstrongly inhibited cell migration. A challenge with 1000 heat-killed bacteria versus 1 epithelial cell resulted in a relative closure of the scratch of 25% forP. gingivalisand 20% forP. nigrescens. Weaker inhibitory effects were found for the other bacteria. The results confirmed our hypothesis that the oral bacteria may be involved in delayed wound healing.

scholarly journals Tannerella forsythia invasion in oral epithelial cells requires phosphoinositide 3-kinase activation and clathrin-mediated endocytosis

Microbiology ◽  
2011 ◽  
Vol 157 (8) ◽  
pp. 2382-2391 ◽  
Author(s):  
Elina Mishima ◽  
Ashu Sharma
Keyword(s):  
Epithelial Cells ◽  
Rho Gtpases ◽  
Membrane Microdomains ◽  
Small Interfering Rnas ◽  
Tannerella Forsythia ◽  
Lipid Kinase ◽  
Signalling Molecules ◽  
Oral Epithelial Cells ◽  
Oral Epithelial

Tannerella forsythia, a Gram-negative anaerobe implicated in periodontitis, has been detected within human buccal epithelial cells and shown to invade oral epithelial cells in vitro. We have previously shown that this bacterium triggers host tyrosine kinase-dependent phosphorylation and actin-dependent cytoskeleton reorganization for invasion. On the bacterial side, the leucine-rich repeat cell-surface BspA protein is important for entry. The present study was undertaken to identify host signalling molecules during T. forsythia entry into human oral and cervical epithelial cells. Specifically, the roles of phosphatidylinositol 3-kinase (PI3K), Rho-family GTPases, cholesterol-rich membrane microdomains and the endocytic protein clathrin were investigated. For this purpose, cell lines were pretreated with chemical inhibitors or small interfering RNAs (siRNAs) that target PI3Ks, Rho GTPases, clathrin and cholesterol (a critical component of ‘lipid rafts’), and the resulting effects on T. forsythia uptake were determined. Our studies revealed that T. forsythia entry is dependent on host PI3K signalling, and that purified BspA protein causes activation of this lipid kinase. Bacterial entry also requires the cooperation of host Rac1 GTPase. Finally, our findings indicate an important role for clathrin and cholesterol-rich lipid microdomains in the internalization process

scholarly journals An In Vitro Model of Ciprofloxacin and Minocycline Transport by Oral Epithelial Cells

2002 ◽  
Vol 73 (11) ◽  
pp. 1267-1272 ◽  
Author(s):  
James J. Brayton ◽  
Qing Yang ◽  
Robin J. Nakkula ◽  
John D. Walters
Keyword(s):  
Epithelial Cells ◽  
In Vitro Model ◽  
Oral Epithelial Cells ◽  
Vitro Model ◽  
Oral Epithelial
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