scholarly journals Efficacy of the Controlled Release of Concentrated Platelet Lysate from a Collagen/Gelatin Scaffold for Dermis-Like Tissue Regeneration

2013 ◽  
Vol 19 (11-12) ◽  
pp. 1398-1405 ◽  
Author(s):  
Ran Ito ◽  
Naoki Morimoto ◽  
Liem Hieu Pham ◽  
Tsuguyoshi Taira ◽  
Katsuya Kawai ◽  
...  
Keyword(s):  
Controlled Release ◽  
Tissue Regeneration ◽  
Platelet Lysate

The Role of a Platelet Lysate-Based Compartmentalized System as a Carrier of Cells and Platelet-Origin Cytokines for Periodontal Tissue Regeneration

2016 ◽  
Vol 22 (19-20) ◽  
pp. 1164-1175 ◽  
Author(s):  
Pedro S. Babo ◽  
Xinjie Cai ◽  
Adelina S. Plachokova ◽  
Rui L. Reis ◽  
John A. Jansen ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Platelet Lysate ◽  
Periodontal Tissue ◽  
Periodontal Tissue Regeneration

scholarly journals Biocomposite nanofibrous strategies for the controlled release of biomolecules for skin tissue regeneration

2014 ◽  
pp. 4709 ◽  
Author(s):  
Dinesh Kumar Srinivasan ◽  
Chinnasamy Gandhimathi ◽  
Jayarama Reddy Venugopal ◽  
Velmurugan Bhaarathy ◽  
Seeram Ramakrishna
Keyword(s):  
Controlled Release ◽  
Tissue Regeneration ◽  
Skin Tissue

Enhanced angiogenesis through controlled release of basic fibroblast growth factor from peptide amphiphile for tissue regeneration

Biomaterials ◽  
2006 ◽  
Vol 27 (34) ◽  
pp. 5836-5844 ◽  
Author(s):  
Hossein Hosseinkhani ◽  
Mohsen Hosseinkhani ◽  
Ali Khademhosseini ◽  
Hisatoshi Kobayashi ◽  
Yasuhiko Tabata
Keyword(s):  
Controlled Release ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Tissue Regeneration ◽  
Fibroblast Growth ◽  
Peptide Amphiphile

scholarly journals Developing a Glyoxal-Crosslinked Chitosan/Gelatin Hydrogel for Sustained Release of Human Platelet Lysate to Promote Tissue Regeneration

2021 ◽  
Vol 22 (12) ◽  
pp. 6451
Author(s):  
Ching-Cheng Tsai ◽  
Tai-Horng Young ◽  
Guang-Shih Chen ◽  
Nai-Chen Cheng
Keyword(s):  
Growth Factor ◽  
Sustained Release ◽  
Tissue Regeneration ◽  
Transforming Growth Factor ◽  
Human Platelet ◽  
Umbilical Vein ◽  
Platelet Lysate ◽  
Release Pattern ◽  
Human Platelet Lysate

The clinical application of human platelet lysate (HPL) holds promise for tissue regeneration, and the development of an efficient vehicle for its delivery is desired. Chitosan-based hydrogels are potential candidates, but they often exhibit weak mechanical properties. In this study, a chitosan/gelatin (CS-GE) hydrogel crosslinked by glyoxal was fabricated for sustained release of HPL. The influence of HPL on Hs68 fibroblast and human umbilical vein endothelial cell (HUVEC) culture was evaluated, and we found that supplementing 5% HPL in the medium could significantly improve cell proliferation relative to supplementing 10% fetal bovine serum (FBS). Moreover, HPL accelerated the in vitro wound closure of Hs68 cells and facilitated the tube formation of HUVECs. Subsequently, we fabricated CS-GE hydrogels crosslinked with different concentrations of glyoxal, and the release pattern of FITC-dextrans (4, 40 and 500 kDa) from the hydrogels was assessed. After an ideal glyoxal concentration was determined, we further characterized the crosslinked CS-GE hydrogels encapsulated with different amounts of HPL. The HPL-incorporated hydrogel was shown to significantly promote the proliferation of Hs68 cells and the migration of HUVECs. Moreover, the release pattern of transforming growth factor-β1 (TGF-β1) and platelet-derived growth factor-BB (PDGF-BB) from hydrogel was examined in vitro, demonstrating a sustained release profile of the growth factors. Finally, the chick chorioallantoic membrane assay revealed that HPL encapsulation in the hydrogel significantly stimulated angiogenesis in ovo. These results demonstrate the great potential of the crosslinked CS-GE hydrogel to serve as an effective delivery system for HPL to promote tissue regeneration.

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