Nonionizing Radiation as a Noninvasive Strategy in Regenerative Medicine: The Effect of Ca2+-ICR on Mouse Skeletal Muscle Cell Growth and Differentiation

2012 ◽  
Vol 18 (21-22) ◽  
pp. 2248-2258 ◽  
Author(s):  
Flavia De Carlo ◽  
Mario Ledda ◽  
Deleana Pozzi ◽  
Pasquale Pierimarchi ◽  
Manuela Zonfrillo ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Growth ◽  
Regenerative Medicine ◽  
Muscle Cell ◽  
Skeletal Muscle Cell ◽  
Mouse Skeletal Muscle ◽  
Nonionizing Radiation ◽  
Cell Growth And Differentiation

scholarly journals Nutritional and hormonal control of skeletal-muscle cell growth and differentiation

1998 ◽  
Vol 57 (02) ◽  
pp. 207-217 ◽  
Author(s):  
Jonh M. Brameld ◽  
Peter J. Buttery ◽  
Janet M. Dawson ◽  
Jane M. M. Harper
Keyword(s):  
Skeletal Muscle ◽  
Cell Growth ◽  
Muscle Cell ◽  
Skeletal Muscle Cell ◽  
Hormonal Control ◽  
Cell Growth And Differentiation

Arachidonic acid supplementation enhances in vitro skeletal muscle cell growth via a COX-2-dependent pathway

2013 ◽  
Vol 304 (1) ◽  
pp. C56-C67 ◽  
Author(s):  
James F. Markworth ◽  
David Cameron-Smith
Keyword(s):  
Skeletal Muscle ◽  
Arachidonic Acid ◽  
Cell Growth ◽  
Muscle Cell ◽  
Myogenic Differentiation ◽  
Skeletal Muscle Cell ◽  
Diverse Range ◽  
Cox 2 ◽  
Dependent Pathway

Arachidonic acid (AA) is the metabolic precursor to a diverse range of downstream bioactive lipid mediators. A positive or negative influence of individual eicosanoid species [e.g., prostaglandins (PGs), leukotrienes, and hydroxyeicosatetraenoic acids] has been implicated in skeletal muscle cell growth and development. The collective role of AA-derived metabolites in physiological states of skeletal muscle growth/atrophy remains unclear. The present study aimed to determine the direct effect of free AA supplementation and subsequent eicosanoid biosynthesis on skeletal myocyte growth in vitro . C2C12 (mouse) skeletal myocytes induced to differentiate with supplemental AA exhibited dose-dependent increases in the size, myonuclear content, and protein accretion of developing myotubes, independent of changes in cell density or the rate/extent of myogenic differentiation. Nonselective (indomethacin) or cyclooxygenase 2 (COX-2)-selective (NS-398) nonsteroidal anti-inflammatory drugs blunted basal myogenesis, an effect that was amplified in the presence of supplemental free AA substrate. The stimulatory effects of AA persisted in preexisting myotubes via a COX-2-dependent (NS-389-sensitive) pathway, specifically implying dependency on downstream PG biosynthesis. AA-stimulated growth was associated with markedly increased secretion of PGF2α and PGE2; however, incubation of myocytes with PG-rich conditioned medium failed to mimic the effects of direct AA supplementation. In vitro AA supplementation stimulates PG release and skeletal muscle cell hypertrophy via a COX-2-dependent pathway.

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