scholarly journals Enhanced Osteogenesis of Human Mesenchymal Stem Cells by Periodic Heat Shock in Self-Assembling Peptide Hydrogel

2013 ◽  
Vol 19 (5-6) ◽  
pp. 716-728 ◽  
Author(s):  
Jing Chen ◽  
Zhong-Dong Shi ◽  
Xinying Ji ◽  
Jorge Morales ◽  
Jingwei Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Heat Shock ◽  
Human Mesenchymal Stem Cells ◽  
Self Assembling ◽  
Periodic Heat ◽  
Peptide Hydrogel

scholarly journals Encapsulin Based Self-Assembling Iron-Containing Protein Nanoparticles for Stem Cells MRI Visualization

2021 ◽  
Vol 22 (22) ◽  
pp. 12275
Author(s):  
Anna N. Gabashvili ◽  
Stepan S. Vodopyanov ◽  
Nelly S. Chmelyuk ◽  
Viktoria A. Sarkisova ◽  
Konstantin A. Fedotov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Viability ◽  
Human Mesenchymal Stem Cells ◽  
Noninvasive Technique ◽  
Self Assembling ◽  
Cargo Protein ◽  
Car T ◽  
New Type ◽  
Magnetic Resonance Imaging Mri

Over the past decade, cell therapy has found many applications in the treatment of different diseases. Some of the cells already used in clinical practice include stem cells and CAR-T cells. Compared with traditional drugs, living cells are much more complicated systems that must be strictly controlled to avoid undesirable migration, differentiation, or proliferation. One of the approaches used to prevent such side effects involves monitoring cell distribution in the human body by any noninvasive technique, such as magnetic resonance imaging (MRI). Long-term tracking of stem cells with artificial magnetic labels, such as magnetic nanoparticles, is quite problematic because such labels can affect the metabolic process and cell viability. Additionally, the concentration of exogenous labels will decrease during cell division, leading to a corresponding decrease in signal intensity. In the current work, we present a new type of genetically encoded label based on encapsulin from Myxococcus xanthus bacteria, stably expressed in human mesenchymal stem cells (MSCs) and coexpressed with ferroxidase as a cargo protein for nanoparticles’ synthesis inside encapsulin shells. mZip14 protein was expressed for the enhancement of iron transport into the cell. Together, these three proteins led to the synthesis of iron-containing nanoparticles in mesenchymal stem cells—without affecting cell viability—and increased contrast properties of MSCs in MRI.

scholarly journals Sublethal heat shock induces premature senescence rather than apoptosis in human mesenchymal stem cells

2013 ◽  
Vol 19 (3) ◽  
pp. 355-366 ◽  
Author(s):  
Larisa L. Alekseenko ◽  
Victoria I. Zemelko ◽  
Alisa P. Domnina ◽  
Olga G. Lyublinskaya ◽  
Valery V. Zenin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Heat Shock ◽  
Human Mesenchymal Stem Cells ◽  
Premature Senescence

scholarly journals Melatonin Enhances Mitophagy by Upregulating Expression of Heat Shock 70 kDa Protein 1L in Human Mesenchymal Stem Cells under Oxidative Stress

2019 ◽  
Vol 20 (18) ◽  
pp. 4545 ◽  
Author(s):  
Yeo Min Yoon ◽  
Hyung Joo Kim ◽  
Jun Hee Lee ◽  
Sang Hun Lee
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Membrane Potential ◽  
Heat Shock ◽  
Mitochondrial Dysfunction ◽  
Defense Mechanisms ◽  
Human Mesenchymal Stem Cells ◽  
Post Transplantation ◽  
Ischemic Disease

Human mesenchymal stem cells (hMSCs) are a potent source of cell-based regenerative therapeutics used to treat patients with ischemic disease. However, disease-induced oxidative stress disrupts mitochondrial homeostasis in transplanted hMSCs, resulting in hMSC apoptosis and reducing their efficacy post-transplantation. To address this issue, we evaluated the effects of melatonin on cellular defense mechanisms and mitophagy in hMSCs subjected to oxidative stress. H2O2-induced oxidative stress increases the levels of reactive oxygen species and reduces membrane potential in hMSCs, leading to mitochondrial dysfunction and cell death. Oxidative stress also decreases the expression of 70-kDa heat shock protein 1L (HSPA1L), a molecular chaperone that assists in the recruitment of parkin to the autophagosomal mitochondrial membrane. Decreased expression of HSPA1L destabilizes parkin, thereby impairing mitophagy. Our results indicate that treating hMSCs with melatonin significantly inhibited mitochondrial dysfunction induced by oxidative stress, which decreased hMSCs apoptosis. In damaged hMSCs, treatment with melatonin increased the levels of HSPA1L, which bound to parkin. The interaction between HSPA1L and parkin increased membrane potential and levels of oxidative phosphorylation, resulting in enhanced mitophagy. Our results indicate that melatonin increased the expression of HSPA1L, thereby upregulating mitophagy and prolonging cell survival under conditions of oxidative stress. In this study, we have shown that melatonin, a readily available compound, can be used to improve hMSC-based therapies for patients with pathologic conditions involving oxidative stress.

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