Effect of Collagen Type I or Type II on Chondrogenesis by Cultured Human Articular Chondrocytes

2013 ◽  
Vol 19 (1-2) ◽  
pp. 59-65 ◽  
Author(s):  
Marijn Rutgers ◽  
Daniel B. Saris ◽  
Lucienne A. Vonk ◽  
Mattie H. van Rijen ◽  
Vanessa Akrum ◽  
...  
Keyword(s):  
Collagen Type ◽  
Articular Chondrocytes ◽  
Collagen Type I ◽  
Human Articular Chondrocytes ◽  
Type I ◽  
Type Ii

Collagen Expression in Tissue Engineered Cartilage of Aged Human Articular Chondrocytes in a Rotating Bioreactor

2003 ◽  
Vol 26 (4) ◽  
pp. 319-330 ◽  
Author(s):  
S. Marlovits ◽  
B. Tichy ◽  
M. Truppe ◽  
D. Gruber ◽  
W. Schlegel
Keyword(s):  
Electron Microscopy ◽  
Collagen Type ◽  
Articular Chondrocytes ◽  
Three Dimensional ◽  
Collagen Type I ◽  
Human Articular Chondrocytes ◽  
Type I ◽  
Transmission Electron ◽  
Low Shear Stress ◽  
Engineered Cartilage

This study describes the culture and three-dimensional assembly of aged human articular chondrocytes under controlled oxygenation and low shear stress in a rotating-wall vessel. Chondrocytes cultured in monolayer were released and placed without any scaffold as a single cell suspension in a rotating bioreactor for 12 weeks. Samples were analyzed with immunohistochemistry, molecular biology and electron microscopy. During serial monolayer cultures chondrocytes dedifferentiated to a “fibroblast-like” structure and produced predominantly collagen type I. When these dedifferentiated cells were transferred to the rotating bioreactor, the cells showed a spontaneous aggregation and formation of solid tissue during the culture time. Expression of collagen type II and other components critical for the extracellular cartilage matrix could be detected. Transmission electron microscopy revealed a fine network of randomly distributed collagen fibrils. This rotating bioreactor proves to be a useful tool for providing an environment that enables dedifferentiated chondrocytes to redifferentiate and produce a cartilage-specific extracellular matrix.

scholarly journals Maintenance of chondrocyte phenotype during expansion on PLLA microtopographies

2018 ◽  
Vol 9 ◽  
pp. 204173141878982 ◽  
Author(s):  
Elisa Costa ◽  
Cristina González-García ◽  
José Luis Gómez Ribelles ◽  
Manuel Salmerón-Sánchez
Keyword(s):  
Lactic Acid ◽  
Type I Collagen ◽  
Collagen Type ◽  
Articular Chondrocytes ◽  
Collagen Type I ◽  
Cell Phenotype ◽  
Type I ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Chondrocyte Phenotype

Articular chondrocytes are difficult to grow, as they lose their characteristic phenotype following expansion on standard tissue culture plates. Here, we show that culturing them on surfaces of poly(L-lactic acid) of well-defined microtopography allows expansion and maintenance of characteristic chondrogenic markers. We investigated the dynamics of human chondrocyte dedifferentiation on the different poly(L-lactic acid) microtopographies by the expression of collagen type I, collagen type II and aggrecan at different culture times. When seeded on poly(L-lactic acid), chondrocytes maintained their characteristic hyaline phenotype up to 7 days, which allowed to expand the initial cell population approximately six times without cell dedifferentiation. Maintenance of cell phenotype was afterwards correlated to cell adhesion on the different substrates. Chondrocytes adhesion occurs via the α5 β1 integrin on poly(L-lactic acid), suggesting cell–fibronectin interactions. However, α2 β1 integrin is mainly expressed on the control substrate after 1 day of culture, and the characteristic chondrocytic markers are lost (collagen type II expression is overcome by the synthesis of collagen type I). Expanding chondrocytes on poly(L-lactic acid) might be an effective solution to prevent dedifferentiation and improving the number of cells needed for autologous chondrocyte transplantation.

Contraction-inducedMmp13and −14expression by goat articular chondrocytes in collagen type I but not type II gels

2011 ◽  
Vol 6 (9) ◽  
pp. 721-730 ◽  
Author(s):  
Agnes D. Berendsen ◽  
Lucienne A. Vonk ◽  
Behrouz Zandieh-Doulabi ◽  
Vincent Everts ◽  
Ruud A. Bank
Keyword(s):  
Collagen Type ◽  
Articular Chondrocytes ◽  
Collagen Type I ◽  
Type I ◽  
Type Ii

A Novel Rat Tail Collagen Type-I Gel for the Cultivation of Human Articular Chondrocytes in Low Cell Density

2007 ◽  
Vol 30 (12) ◽  
pp. 1057-1067 ◽  
Author(s):  
R. Muller-Rath ◽  
K. Gavénis ◽  
S. Andereya ◽  
T. Mumme ◽  
B. Schmidt-Rohlfing ◽  
...  
Keyword(s):  
Cell Density ◽  
Collagen Type ◽  
Articular Chondrocytes ◽  
Collagen Type I ◽  
Human Articular Chondrocytes ◽  
Type I ◽  
Low Cell Density ◽  
Rat Tail

scholarly journals Millicurrent stimulation of human articular chondrocytes cultivated in a collagen type-I gel and of human osteochondral explants

2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Karsten Gavénis ◽  
Stefan Andereya ◽  
Bernhard Schmidt-Rohlfing ◽  
Ralf Mueller-Rath ◽  
Jiri Silny ◽  
...  
Keyword(s):  
Collagen Type ◽  
Articular Chondrocytes ◽  
Collagen Type I ◽  
Human Articular Chondrocytes ◽  
Type I ◽  
Stimulation Of

Limb bud mesenchyme cultured under tensile strain remodel collagen type I tubes to produce fibrillar collagen type II

Biorheology ◽  
2009 ◽  
Vol 46 (6) ◽  
pp. 439-450 ◽  
Author(s):  
Jennifer R. Amos ◽  
Shigeng Li ◽  
Michael Yost ◽  
Harry Phloen ◽  
Jay D. Potts
Keyword(s):  
Tensile Strain ◽  
Collagen Type ◽  
Collagen Type I ◽  
Limb Bud ◽  
Type I ◽  
Fibrillar Collagen ◽  
Type Ii ◽  
Collagen Type Ii

Limb bud mesenchyme cultured under tensile strain remodel collagen type I tubes to produce fibrillar collagen type II

Biorheology ◽  
2010 ◽  
Vol 47 (2) ◽  
pp. 163-163
Author(s):  
Jennifer R. Amos ◽  
Shigeng Li ◽  
Michael Yost ◽  
Harry Phloen ◽  
Jay D. Potts
Keyword(s):  
Tensile Strain ◽  
Collagen Type ◽  
Collagen Type I ◽  
Limb Bud ◽  
Type I ◽  
Fibrillar Collagen ◽  
Type Ii ◽  
Collagen Type Ii

scholarly journals Light and electron microscopicin situhybridization of collagen type I and type II mRNA in the fibrocartilaginous tissue of late-stage osteoarthritis

1998 ◽  
Vol 6 (4) ◽  
pp. 278-285 ◽  
Author(s):  
Nicolai Miosge ◽  
Kai Waletzko ◽  
Christa Bode ◽  
Fabio Quondamatteo ◽  
Wolfgang Schultz ◽  
...  
Keyword(s):  
Late Stage ◽  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Type Ii

Optimization of Histoprocessing Methods to Detect Glycosaminoglycan, Collagen Type II, and Collagen Type I in Decalcified Rabbit Osteochondral Sections

2005 ◽  
Vol 28 (3) ◽  
pp. 165-175 ◽  
Author(s):  
Anik Chevrier ◽  
Evgeny Rossomacha ◽  
Michael D. Buschmann ◽  
Caroline D. Hoemann
Keyword(s):  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Type Ii ◽  
Collagen Type Ii
Sign in / Sign up

Export Citation Format

Share Document