Fibrin Glue with Autogenic Bone Marrow Mesenchymal Stem Cells for Urethral Injury Repair in Rabbit Model

2012 ◽  
Vol 18 (23-24) ◽  
pp. 2507-2517 ◽  
Author(s):  
Kai Wang ◽  
Yong Guan ◽  
Yi Liu ◽  
Meifeng Zhu ◽  
Ting Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Fibrin Glue ◽  
Rabbit Model ◽  
Urethral Injury ◽  
Injury Repair ◽  
Marrow Mesenchymal Stem Cells

microRNA-265 Regulates Bone Marrow Mesenchymal Stem Cells Differentiation and Promotes Sepsis Lung Injury Repair via Transforming Growth Factor Beta 1

2022 ◽  
Vol 12 (2) ◽  
pp. 405-410
Author(s):  
Lian Tan ◽  
Xiongxiong Wang ◽  
Danqi Chen ◽  
Li Xu ◽  
Yudong Xu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Lung Injury ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Alveolar Type ◽  
Injury Repair ◽  
Qrt Pcr ◽  
Marrow Mesenchymal Stem Cells

Our study investigates whether miR-265 regulates the differentiation of rat bone marrow mesenchymal stem cells (BMSCs) into alveolar type II epithelial cells (ATII) through TGF-β1 and promotes lung injury repair in rats with sepsis, thereby inhibiting sepsis progression. 25 patients with sepsis admitted to the Respiratory and Critical Care Medicine Department of the hospital and 17 normal controls were included. TGF-β1 level was measured by ELISA. miR-265 level was measured by qRT-PCR and AT II-related genes and proteins expression was analyzed by western blot and qRT-PCR. miR-265 expression was significantly higher in sepsis patients than normal group. Progenitor BMSCs were long and shuttle-shaped after 1 and 3 days of growth. Cultured MSCs had low expression of the negative antigen CD34 (4.32%) and high expression of the positive antigen CD44 (99.87%). TGF-β1 level was significantly increased with longer induction time, while miR-265 expression was significantly decreased in cell culture medium. miR-265 interference significantly decreased TGF-β1 expression. In conclusion, miR-265 inhibits BMSC differentiation to AT II via regulation of TGF-β1, thereby inhibiting sepsis progression.

EVALUATION OF THREE DIMENSIONAL CONSTRUCT ENGINEERED FROM POLY(LACTIC-CO-GLYCOLIC ACID)/FIBRIN HYBRID SCAFFOLD USING RABBIT BONE MARROW MESENCHYMAL STEM CELLS FOR OSTEOCHONDRAL DEFECT REPAIR

2015 ◽  
Vol 77 (25) ◽  
Author(s):  
Rozlin Abdul Rahman ◽  
Norhamiza Mohamad Sukri ◽  
Noorhidayah Md Nazir ◽  
Muhammad Aa’zamuddin Ahmad Radzi ◽  
Ahmad Hafiz Zulkifly ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Glycolic Acid ◽  
Osteochondral Defect ◽  
Rabbit Model ◽  
Three Dimensional ◽  
Marker Genes ◽  
Repair Capacity ◽  
Marrow Mesenchymal Stem Cells

Articular cartilage has poor repair capacity due to its avascular and aneural properties and has relatively few cells. This study investigated the ability of autologous implantation approach using three dimensional (3D) constructs engineered from bone marrow mesenchymal stem cells (BMSCs) seeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin as cells carrier for the repair of osteochondral defect in rabbit model. The engineered 3D constructs – PLGA/Fibrin/BMSCs and PLGA/BMSCs – were cultured for 3 weeks in vitro and implanted autologously to the osteochondral defect created in the rabbit knee. The in vivo constructs were harvested and evaluated by means of gross observation, histology assessment, gene expression study, sulphated glycosaminoglycan (sGAG) production assay and biomechanical evaluation at 6 and 12 weeks post implantation. The results showed that the osteochondral defects treated with the PLGA/Fibrin/BMSCs constructs exhibited better repairment, more cartilaginous extracellular matrix, higher sGAG production, superior compressive strength and more intense expression of chondrogenic marker genes than the PLGA/BMSCs group. This study suggested that the PLGA/Fibrin/BMSCs has the potential to treat osteochondral defect and may be presented as a viable therapeutic option for those who would be in need from the life-extending benefits of tissue replacement or repair.

Survival of bone marrow mesenchymal stem cells labelled with red fluorescent protein in an ovine model of collagenase-induced tendinitis

2014 ◽  
Vol 27 (03) ◽  
pp. 204-209 ◽  
Author(s):  
F. Staffieri ◽  
G. Rossi ◽  
E. Francioso ◽  
A. Crovace ◽  
L. Lacitignola
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Fibrin Glue ◽  
Fluorescent Protein ◽  
Allogeneic Bone ◽  
Red Fluorescent Protein ◽  
Ovine Model ◽  
Cd34 Cells ◽  
Marrow Mesenchymal Stem Cells

SummaryObjective: The aim of this study was to track the survival and efficacy of allogeneic bone marrow mesenchymal stem cells (BM-MSC) marked with red fluorescent protein (BMMSCRFP) in an ovine model of collagenase-induced tendinopathy.Methods: Bone marrow was harvested from one donor sheep and BM-MSC were isolated, cultivated and transfected with red fluorescent protein (BM-MSCRFP). Collagenase was injected into both Achilles tendons in the remaining nine sheep. After two weeks the left tendon was injected with a solution of 6 x 106 BM-MSCRFP and fibrin glue, while only fibrin glue was administered to the contralateral tendon in each sheep. After three, four and six weeks the tendons were harvested and evaluated for morphology, collagen I deposition, presence of CD34+ cells, and fluorescent labelled BM-MSC.Results: We demonstrated that delivery of BM-MSC into tendon lesions had positive effects on the injured tendons. The BM-MSCRFP survived at three, four and six weeks after treatment, leading to better quality healing of tendons as compared to the controls, where no labelled cells were detected. Interestingly, we demonstrated high expression of CD34+ cells in tendons that had been treated with BM-MSCRFP.Clinical relevance: Mesenchymal stem cell allografts have a positive effect on tendon healing and local injection of BM-MSC directly into the tendon allows the homing of BM-MSC for good efficiency of engraftment.

Bone Marrow Mesenchymal Stem Cells Induce Angiogenesis and Promote Bladder Cancer Growth in a Rabbit Model

2010 ◽  
Vol 84 (1) ◽  
pp. 94-99 ◽  
Author(s):  
Keqin Zhang ◽  
Benkang Shi ◽  
Jun Chen ◽  
Dongqing Zhang ◽  
Yaofeng Zhu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bladder Cancer ◽  
Mesenchymal Stem Cells ◽  
Rabbit Model ◽  
Cancer Growth ◽  
Marrow Mesenchymal Stem Cells
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