Discontinuous Release of Bone Morphogenetic Protein-2 Loaded Within Interconnected Pores of Honeycomb-Like Polycaprolactone Scaffold Promotes Bone Healing in a Large Bone Defect of Rabbit Ulna

2011 ◽  
Vol 17 (19-20) ◽  
pp. 2389-2397 ◽  
Author(s):  
Ji-Hoon Bae ◽  
Hae-Ryong Song ◽  
Hak-Jun Kim ◽  
Hong-Chul Lim ◽  
Jung-Ho Park ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Defect ◽  
Bone Healing ◽  
Bone Morphogenetic Protein 2 ◽  
Large Bone Defect ◽  
Morphogenetic Protein ◽  
Large Bone ◽  
Interconnected Pores

scholarly journals Effects of Bone Morphogenetic Protein-2 on Neovascularization During Large Bone Defect Regeneration

2019 ◽  
Vol 25 (23-24) ◽  
pp. 1623-1634 ◽  
Author(s):  
Hope B. Pearson ◽  
Devon E. Mason ◽  
Christopher D. Kegelman ◽  
Liming Zhao ◽  
James H. Dawahare ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Defect ◽  
Bone Morphogenetic Protein 2 ◽  
Large Bone Defect ◽  
Morphogenetic Protein ◽  
Large Bone

scholarly journals Poly(POG)n loaded with recombinant human bone morphogenetic protein-2 accelerates new bone formation in a critical-sized bone defect mouse model

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Ryo Tazawa ◽  
Kentaro Uchida ◽  
Hiroaki Minehara ◽  
Terumasa Matsuura ◽  
Tadashi Kawamura ◽  
...  
Keyword(s):  
Mouse Model ◽  
Bone Formation ◽  
Bone Morphogenetic Protein ◽  
Bone Defect ◽  
Bone Defects ◽  
Bone Morphogenetic Protein 2 ◽  
New Bone Formation ◽  
Morphogenetic Protein ◽  
Large Bone ◽  
Large Bone Defects

Abstract Background Delivery of bone morphogenetic protein-2 (BMP-2) via animal-derived absorbable collagen materials is used for the treatment of large bone defects. However, the administration of bovine proteins to humans is associated with the risk of zoonotic complications. We therefore examined the effect of combining BMP-2 with collagen-like peptides, poly(POG)n, in a critical-sized bone defect mouse model. Methods A 2-mm critical-sized bone defect was created in the femur of 9-week-old male C57/BL6J mice. Mice were randomly allocated into one of four treatment groups (n = 6 each): control (no treatment), poly(POG)n only, 0.2 μg, or 2.0 μg BMP-2 with poly(POG)n. New bone formation was monitored using soft X-ray radiographs, and bone formation at the bone defect site was examined using micro-computed tomography and histological examination at 4 weeks after surgery. Results Administration of 2.0 μg of BMP-2 with poly(POG)n promoted new bone formation and resulted in greater bone volume and bone mineral content than that observed in the control group and successfully achieved consolidation. In contrast, bone formation in all other groups was scarce. Conclusions Our findings suggest the potential of BMP-2 with poly(POG)n as a material, free from animal-derived collagen, for the treatment of large bone defects.

scholarly journals Bone morphogenetic protein-2-impregnated biomimetic scaffolds successfully induce bone healing in a marginal mandibular defect

2013 ◽  
Vol 123 (5) ◽  
pp. 1149-1155 ◽  
Author(s):  
Adam S. DeConde ◽  
Douglas Sidell ◽  
Min Lee ◽  
Olga Bezouglaia ◽  
Kyle Low ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Healing ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Biomimetic Scaffolds ◽  
Mandibular Defect

Enhancement of Bone Healing Based onEx VivoGene Therapy Using Human Muscle-Derived Cells Expressing Bone Morphogenetic Protein 2

2002 ◽  
Vol 13 (10) ◽  
pp. 1201-1211 ◽  
Author(s):  
Joon Yung Lee ◽  
Hairong Peng ◽  
Arvydas Usas ◽  
Douglas Musgrave ◽  
James Cummins ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Healing ◽  
Human Muscle ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein

scholarly journals Effect of Stromal Vascular Fraction on Fracture Healing with Bone Defects by Examination of Bone Morphogenetic Protein-2 Biomarkers in Murine Model

2021 ◽  
Vol 9 (A) ◽  
pp. 1132-1136
Author(s):  
Respati S. Dradjat ◽  
Panji Sananta ◽  
Rizqi Daniar Rosandi ◽  
Lasa Dhakka Siahaan
Keyword(s):  
Bone Formation ◽  
Bone Morphogenetic Protein ◽  
Bone Defect ◽  
Murine Model ◽  
Healing Process ◽  
Stromal Vascular Fraction ◽  
Bone Defects ◽  
The Body ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein

BACKGROUND: Fractures and segmental bone defects are a significant cause of morbidity and a source of a high economic burden in healthcare. A severe bone defect (3 mm in murine model) is a devastating condition, which the bone cannot heal naturally despite surgical stabilization and usually requires further surgical intervention. The stromal vascular fraction (SVF) contains a heterogeneous collection of cells and several components, primarily: MSCs, HSCs, Treg cells, pericytic cells, AST cells, extracellular matrix, and complex microvascular beds (fibroblasts, white blood cells, dendritic cells, and intra-adventitial smooth muscular-like cells). Bone morphogenetic protein (BMP) is widely known for their important role in bone formation during mammalian development and confers a multifunctional role in the body, which has potential for therapeutic use. Studies have shown that BMPs play a role in the healing of large size bone defects. AIM: In this study, researchers aim to determine the effect of administering SVF from adipose tissue on the healing process of bone defects assessed based on the level biomarker of BMP-2. MATERIALS AND METHODS: This was an animal study involving 12 Wistar strain Rattus norvegivus. They were divided into three groups: Negative group (normal rats), positive group (rats with bone defect without SVF application), and SVF group (rats with bone defect with SVF application). After 30 days, the rats were sacrificed; the biomarkers that were evaluated are BMP-2. This biomarker was quantified using ELISA. RESULTS: BMP-2 biomarker expressions were higher in the SVF application group than in the group without SVF. All comparisons of the SVF group and positive control group showed significant differences (p = 0.026). CONCLUSION: SVF application could aid the healing process in a murine model with bone defect marked by the increased level of BMP-2 as a bone formation marker.

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