Dose- and Time-Dependent Effects of Cyclic Hydrostatic Pressure on Transforming Growth Factor-β3-Induced Chondrogenesis by Adult Human Mesenchymal Stem Cells in Vitro

2006 ◽  
Vol 12 (8) ◽  
pp. 2253-2262 ◽  
Author(s):  
Keita Miyanishi ◽  
Michael C.D. Trindade ◽  
Derek P. Lindsey ◽  
Gary S. Beaupré ◽  
Dennis R. Carter ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Hydrostatic Pressure ◽  
Transforming Growth Factor ◽  
Human Mesenchymal Stem Cells ◽  
Time Dependent ◽  
Adult Human

Dose- and Time-Dependent Effects of Cyclic Hydrostatic Pressure on Transforming Growth Factor-3-Induced Chondrogenesis by Adult Human Mesenchymal Stem Cells in Vitro

2006 ◽  
Vol 0 (0) ◽  
pp. 060706073730066
Author(s):  
Keita Miyanishi ◽  
Michael C.D. Trindade ◽  
Derek P. Lindsey ◽  
Gary S. Beaupre ◽  
Dennis R. Carter ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Hydrostatic Pressure ◽  
Transforming Growth Factor ◽  
Human Mesenchymal Stem Cells ◽  
Time Dependent ◽  
Adult Human

Effects of a bone-like mineral film on phenotype of adult human mesenchymal stem cells in vitro

Biomaterials ◽  
2005 ◽  
Vol 26 (3) ◽  
pp. 303-310 ◽  
Author(s):  
W MURPHY ◽  
S HSIONG ◽  
T RICHARDSON ◽  
C SIMMONS ◽  
D MOONEY
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Adult Human

Effects of Hydrostatic Pressure and Transforming Growth Factor-β3 on Adult Human Mesenchymal Stem Cell Chondrogenesis In Vitro

2006 ◽  
Vol 12 (6) ◽  
pp. 1419-1428 ◽  
Author(s):  
Keita Miyanishi ◽  
Michael C.D. Trindade ◽  
Derek P. Lindsey ◽  
Gary S. Beaupré ◽  
Dennis R. Carter ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Hydrostatic Pressure ◽  
Mesenchymal Stem Cell ◽  
Transforming Growth Factor ◽  
Human Mesenchymal Stem Cell ◽  
Adult Human

EFFECTS OF SHORT-TERM CYCLIC HYDROSTATIC PRESSURE ON INITIATING AND ENHANCING THE EXPRESSION OF CHONDROGENIC GENES IN HUMAN ADIPOSE-DERIVED MESENCHYMAL STEM CELLS

2014 ◽  
Vol 14 (04) ◽  
pp. 1450054 ◽  
Author(s):  
FARZANEH SAFSHEKAN ◽  
MOHAMMAD TAFAZZOLI SHADPOUR ◽  
MOHAMMAD ALI SHOKRGOZAR ◽  
NOOSHIN HAGHIGHIPOUR ◽  
SEYED HAMED ALAVI
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Hydrostatic Pressure ◽  
Chondrogenic Differentiation ◽  
Negative Control ◽  
Cartilage Tissue ◽  
Control Group

Cartilage tissue engineering is a promising treatment for damaged or diseased cartilage that requires thorough understanding of influential parameters involved in chondrogenic differentiation. This study examined how 4-h application of cyclic hydrostatic pressure (CHP) of 5 MPa at 0.5 Hz could modulate chondroinduction of human adipose-derived mesenchymal stem cells (hAMSCs) in vitro. Four groups were examined including a negative control group, a chemical group treated by growth factor for 10 days, a mechanical group exposed to 4-h loading on the 10th day of pellet culture without any chondrogenic stimulator, and finally a chemical-mechanical group subjected to both growth factor and loading. Application of cyclic hydrostatic pressure increased the expression of chondrogenic genes, including sox9 and aggrecan to higher levels than those of the chemical group. This study indicates that cyclic hydrostatic pressure initiates and enhances the chondrogenic differentiation of mesenchymal stem cells with or without growth factors in vitro and confirms the important role of hydrostatic pressure during chondrogenesis in vivo.

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