Transgene Expression Level and Inherent Differences in Target Gene Activation Determine the Rate and Fate of Neurogenin3-Mediated Islet Cell Differentiation In Vitro

2007 ◽  
Vol 13 (4) ◽  
pp. 775-788 ◽  
Author(s):  
Michael I. Boretti ◽  
Keith J. Gooch
Keyword(s):  
Cell Differentiation ◽  
Islet Cell ◽  
Transgene Expression ◽  
Target Gene ◽  
Gene Activation ◽  
Expression Level ◽  
Transgene Expression Level

scholarly journals Increased transgene expression level of rabies virus vector for transsynaptic tracing

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0180960 ◽  
Author(s):  
Shinya Ohara ◽  
Yasuhiro Sota ◽  
Sho Sato ◽  
Ken-Ichiro Tsutsui ◽  
Toshio Iijima
Keyword(s):  
Rabies Virus ◽  
Transgene Expression ◽  
Virus Vector ◽  
Expression Level ◽  
Transsynaptic Tracing ◽  
Transgene Expression Level

scholarly journals High transgene expression is associated with systemic GFP silencing in Nicotiana benthamiana

2021 ◽  
Author(s):  
Bill Hendrix ◽  
Paul Hoffer ◽  
Rick Sanders ◽  
Steve Schwartz ◽  
Wei Zheng ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Nicotiana Benthamiana ◽  
Transgene Expression ◽  
Fluorescent Protein ◽  
Integration Site ◽  
Crop Protection ◽  
Transgene Silencing ◽  
Expression Level ◽  
Transgenic Lines ◽  
Transgene Expression Level

AbstractGene silencing in plants using topical dsRNA is a new approach that has the potential to be a sustainable component of the agricultural production systems of the future. However, more research is needed to enable this technology as an economical and efficacious supplement to current crop protection practices. Systemic gene silencing is one key enabling aspect. The objective of this research was to better understand systemic transgene silencing in Nicotiana benthamiana. Previous reports details sequencing of the integration site of the Green Fluorescent Protein (GFP) transgene in the well-known N. benthamiana GFP16C event revealed inadvertent co-integration of part of a bacterial transposase. To determine the effect of this transgene configuration on systemic silencing, new GFP transgenic lines with or without the transposase sequences were produced. GFP expression levels in the 19 single-copy events and three hemizygous 16C lines produced for this study ranged from 50-72% of the homozygous 16C line. GFP expression was equivalent to 16C in a two-copy event. Local GFP silencing was observed in all transgenic and 16C hemizygous lines after topical application of delivery formulations with a GFP targeting dsRNA. The 16C-like systemic silencing phenotype was only observed in the two-copy line. The partial transposase had no impact on transgene expression level, local GFP silencing, small RNA abundance and distribution, or systemic GFP silencing in the transgenic lines. We conclude that high transgene expression level is a key enabler of systemic transgene silencing in N. benthamiana.

scholarly journals MicroRNA-122a Regulates Zonulin by Targeting EGFR in Intestinal Epithelial Dysfunction

2017 ◽  
Vol 42 (2) ◽  
pp. 848-858 ◽  
Author(s):  
Bin Zhang ◽  
Yinghai Tian ◽  
Ping Jiang ◽  
Yanqiong Jiang ◽  
Chao Li ◽  
...  
Keyword(s):  
Target Gene ◽  
Growth Factor Receptor ◽  
Intestinal Barrier ◽  
Expression Level ◽  
Intestinal Epithelial ◽  
Barrier Methods ◽  
Clinical Specimens ◽  
Epithelial Culture

Background/Aims: This study aimed to investigate the role of microRNA (miR)-122a in regulating zonulin during the modulation of intestinal barrier. Methods: Zonulin proteins and their target gene expression were analyzed in miR-122a-overexpressing cell lines and in the target gene of epidermal growth factor receptor (EGFR). An mmu-miR-122a intestinal epithelial conditional transgenic (miR-122a-TG) mouse model was established to investigate EGFR and zonulin expression. MiR-122a was also detected in the clinical specimens of inflammatory bowel disease. Results: EGFR was identified as a target gene of miR-122a. The expression level of miR-122a was positively correlated with that of zonulin. The expression level of zonulin was significantly increased, whereas the expression level of EGFR was significantly decreased in the miR-122a-TG mice and in the corresponding primary epithelial culture (P < 0.05). These results were consistent with the data of the clinical specimens. Conclusions: miR-122a could be a positive factor of zonulin by targeting EGFR, which increased the intestinal epithelial permeability in vivo and in vitro.

scholarly journals Rabies Virus Vector Transgene Expression Level and Cytotoxicity Improvement Induced by Deletion of Glycoprotein Gene

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e80245 ◽  
Author(s):  
Shinya Ohara ◽  
Sho Sato ◽  
Kei Oyama ◽  
Ken-Ichiro Tsutsui ◽  
Toshio Iijima
Keyword(s):  
Rabies Virus ◽  
Transgene Expression ◽  
Virus Vector ◽  
Expression Level ◽  
Glycoprotein Gene ◽  
Transgene Expression Level

scholarly journals Enhancers with cooperative Notch binding sites are more resistant to regulation by the Hairless co-repressor

PLoS Genetics ◽  
2021 ◽  
Vol 17 (9) ◽  
pp. e1009039
Author(s):  
Yi Kuang ◽  
Anna Pyo ◽  
Natanel Eafergan ◽  
Brittany Cain ◽  
Lisa M. Gutzwiller ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Binding Sites ◽  
Target Gene ◽  
Gene Activation ◽  
Notch Intracellular Domain ◽  
Repressor Complex ◽  
Notch Activation

Notch signaling controls many developmental processes by regulating gene expression. Notch-dependent enhancers recruit activation complexes consisting of the Notch intracellular domain, the Cbf/Su(H)/Lag1 (CSL) transcription factor (TF), and the Mastermind co-factor via two types of DNA sites: monomeric CSL sites and cooperative dimer sites called Su(H) paired sites (SPS). Intriguingly, the CSL TF can also bind co-repressors to negatively regulate transcription via these same sites. Here, we tested how synthetic enhancers with monomeric CSL sites versus dimeric SPSs bind Drosophila Su(H) complexes in vitro and mediate transcriptional outcomes in vivo. Our findings reveal that while the Su(H)/Hairless co-repressor complex similarly binds SPS and CSL sites in an additive manner, the Notch activation complex binds SPSs, but not CSL sites, in a cooperative manner. Moreover, transgenic reporters with SPSs mediate stronger, more consistent transcription and are more resistant to increased Hairless co-repressor expression compared to reporters with the same number of CSL sites. These findings support a model in which SPS containing enhancers preferentially recruit cooperative Notch activation complexes over Hairless repression complexes to ensure consistent target gene activation.

scholarly journals Retinoid Target Gene Activation during Induced Tumor Cell Differentiation: Human Embryonal Carcinoma as a Model

2003 ◽  
Vol 133 (1) ◽  
pp. 273S-276S ◽  
Author(s):  
Michael J. Spinella ◽  
Joanna S. Kerley ◽  
Kristina A. White ◽  
Joshua C. Curtin
Keyword(s):  
Cell Differentiation ◽  
Tumor Cell ◽  
Target Gene ◽  
Embryonal Carcinoma ◽  
Gene Activation ◽  
Tumor Cell Differentiation
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