Culture-Expanded, Bone Marrow-Derived Mesenchymal Stem Cells Can Regenerate a Critical-Sized Segmental Bone Defect

1997 ◽  
Vol 3 (2) ◽  
pp. 173-185 ◽  
Author(s):  
Sudha Kadiyala ◽  
Neelam Jaiswal ◽  
Scott P. Bruder
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Defect ◽  
Segmental Bone Defect ◽  
Segmental Bone

scholarly journals Mobilization of Transplanted Bone Marrow Mesenchymal Stem Cells by Erythropoietin Facilitates the Reconstruction of Segmental Bone Defect

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Jun Li ◽  
Zeyu Huang ◽  
Bohua Li ◽  
Zhengdong Zhang ◽  
Lei Liu
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Defect ◽  
Bone Repair ◽  
Self Healing ◽  
Mineral Density ◽  
Segmental Bone Defect ◽  
Marrow Mesenchymal Stem Cells ◽  
Segmental Bone

Reconstruction of segmental bone defects poses a tremendous challenge for both orthopedic clinicians and scientists, since bone rehabilitation is requisite substantially and may be beyond the capacity of self-healing. Bone marrow mesenchymal stem cells (BMSCs) have been identified as an optimal progenitor cell source to facilitate bone repair since they have a higher ability for proliferation and are more easily accessible than mature osteoblastic cells. In spite of the potential of BMSCs in regeneration medicine, particularly for bone reconstruction, noteworthy limitations still remain in previous application of BMSCs, including the amount of cells that could be recruited, the compromised bone migration of grafted cells, reduced proliferation and osteoblastic differentiation ability, and likely tumorigenesis. Our current work demonstrates that BMSCs transplanted through the caudal vein can be mobilized by erythropoietin (EPO) to the bone defect area and participate in regeneration of new bone. Based on the histological analysis and micro-CT findings of this study, EPO can dramatically promote the effects on the osteogenesis and angiogenesis efficiency of BMSCs in vivo. Animals that underwent EPO+BMSC administration demonstrated a remarkable increase in new bone formation, tissue structure organization, new vessel density, callus formation, and bone mineral density (BMD) compared with the BMSCs alone and control groups. At the biomechanical level, we demonstrated that combing transplantation of EPO and BMSCs enhances bone defect reconstruction by increasing the strength of the diaphysis, making it less fragile. Therefore, combination therapy using EPO infusion and BMSC transplantation may be a new therapeutic strategy for the reconstruction of segmental bone defect.

Repairing goat tibia segmental bone defect using scaffold cultured with mesenchymal stem cells

2010 ◽  
Vol 9999B ◽  
pp. NA-NA ◽  
Author(s):  
Xinhui Liu ◽  
Xiaoming Li ◽  
Yubo Fan ◽  
Guoping Zhang ◽  
Dongmei Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Defect ◽  
Segmental Bone Defect ◽  
Segmental Bone

scholarly journals Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration

2021 ◽  
Vol 9 ◽  
Author(s):  
Jun Li ◽  
Wenzhao Wang ◽  
Mingxin Li ◽  
Ping Song ◽  
Haoyuan Lei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue ◽  
Bone Defect ◽  
Bone Defects ◽  
Biomimetic Materials ◽  
Marrow Mesenchymal Stem Cells ◽  
Segmental Bone

Large-segment bone defect caused by trauma or tumor is one of the most challenging problems in orthopedic clinics. Biomimetic materials for bone tissue engineering have developed dramatically in the past few decades. The organic combination of biomimetic materials and stem cells offers new strategies for tissue repair, and the fate of stem cells is closely related to their extracellular matrix (ECM) properties. In this study, a photocrosslinked biomimetic methacrylated gelatin (Bio-GelMA) hydrogel scaffold was prepared to simulate the physical structure and chemical composition of the natural bone extracellular matrix, providing a three-dimensional (3D) template and extracellular matrix microenvironment. Bone marrow mesenchymal stem cells (BMSCS) were encapsulated in Bio-GelMA scaffolds to examine the therapeutic effects of ECM-loaded cells in a 3D environment simulated for segmental bone defects. In vitro results showed that Bio-GelMA had good biocompatibility and sufficient mechanical properties (14.22kPa). A rat segmental bone defect model was constructed in vivo. The GelMA-BMSC suspension was added into the PDMS mold with the size of the bone defect and photocured as a scaffold. BMSC-loaded Bio-GelMA resulted in maximum and robust new bone formation compared with hydrogels alone and stem cell group. In conclusion, the bio-GelMA scaffold can be used as a cell carrier of BMSC to promote the repair of segmental bone defects and has great potential in future clinical applications.

Sign in / Sign up

Export Citation Format

Share Document