Experimental Characterization and Control of an Impinging Jet Issued from a Rocket Nozzle

New Space ◽  
2021 ◽  
Author(s):  
Yogesh Mehta ◽  
Vikas N. Bhargav ◽  
Rajan Kumar
Keyword(s):  
Impinging Jet ◽  
Experimental Characterization ◽  
Rocket Nozzle ◽  
And Control

scholarly journals Automated design of highly diverse riboswitches

2019 ◽  
Author(s):  
Michelle J Wu ◽  
Johan O L Andreasson ◽  
Wipapat Kladwang ◽  
William Greenleaf ◽  
Rhiju Das
Keyword(s):  
Molecular Machines ◽  
Experimental Tests ◽  
Automated Design ◽  
Massively Parallel ◽  
Flavin Mononucleotide ◽  
Experimental Characterization ◽  
Automated Method ◽  
Array Technology ◽  
And Control ◽  
Control Designs

AbstractRiboswitches that couple binding of ligands to recruitment of molecular machines offer sensors and control elements for RNA synthetic biology and medical biotechnology. Current approaches to riboswitch design enable significant changes in output activity in the presence vs. absence of input ligands. However, design of these riboswitches has so far required expert intuition and explicit specification of complete target secondary structures, both of which limit the structure-toggling mechanisms that have been explored. We present a fully automated method called RiboLogic for these design tasks and high-throughput experimental tests of 2,875 molecules using RNA-MaP (RNA on a massively parallel array) technology. RiboLogic designs explore an unprecedented diversity of structure-toggling mechanisms validated through experimental tests. These synthetic molecules consistently modulate their affinity to the MS2 bacteriophage coat protein upon binding of flavin mononucleotide, tryptophan, theophylline, and microRNA miR-208a, achieving activation ratios of up to 20 and significantly better performance than control designs. The data enable dissection of features of structure-toggling mechanisms that correlate with higher performance. The diversity of RiboLogic designs and their quantitative experimental characterization provides a rich resource for further improvement of riboswitch models and design methods.

Defects in Materials: Their Characterization and Simulation.

1990 ◽  
Vol 209 ◽  
Author(s):  
Colin G. Windsor
Keyword(s):  
Point Defects ◽  
Uranium Oxide ◽  
Computer Modelling ◽  
Experimental Characterization ◽  
Dynamic Measurements ◽  
Stress Concentrations ◽  
Copper Clusters ◽  
Materials Research ◽  
Complementary Nature ◽  
And Control

ABSTRACTMaterials research does not necessarily need to eliminate defects, but rather to characterize them, and to understand and control their effects. In most cases chacterization of defects means making structural or dynamic measurements of their properties. To understand these measurements in order to predict material and defect properties outside the range of the measurements is a much harder problem. Ideally a theory is required. However in the materials examples considered in this review, point defects in uranium oxide, copper clusters in steel, grain boundary aggregations, and stress concentrations, a true analytic theory is beyond our capabilities. Here computer modelling is often able to make the progress needed. This review considers the complementary nature of experimental characterization and computer simulation in our understanding of defects in materials.

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