IL-1α Regulates Osteogenesis and Osteoclastic Activity of Dental Follicle Cells Through JNK and p38 MAPK Pathways

2020 ◽  
Vol 29 (24) ◽  
pp. 1552-1566
Author(s):  
Mingmei Meng ◽  
Yandi Chen ◽  
Xinlei Chen ◽  
Qiong Zhang ◽  
Weihua Guo ◽  
...  
Keyword(s):  
P38 Mapk ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Osteoclastic Activity ◽  
Mapk Pathways

IL-1α regulates osteogenic differentiation and osteoclastic activity of dental follicle cells via JNK and p38 MAPK pathways

2020 ◽  
Author(s):  
mingmei meng ◽  
Yandi Chen ◽  
Xinlei Chen ◽  
Qiong Zhang ◽  
Weihua Guo ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
P38 Mapk ◽  
Mapk Signaling ◽  
Tooth Eruption ◽  
Follicle Cells ◽  
Signal Pathways ◽  
Matrix Remodeling ◽  
Dental Follicle ◽  
Osteoclastic Activity ◽  
Specific Inhibitors

Abstract Background The expression of interleukin-1α (IL-1α) is increased in tooth with periapical lesion and the eruption of the permanent successor is accelerated in the presence of an infected deciduous tooth with abscess formation. Dental follicle (DF) plays a central role in tooth eruption through regulating alveolar bone resorption and formation. However, whether the increased IL-1α can disturb functions of DF and influence tooth eruption remains unknown. Herein, we studied the effect and possible mechanism of IL-1α on osteogenic differentiation, osteoclastic activity and matrix remodeling of the DF cells (DFCs). Methods Rat DFCs were treated with IL-1α. Quantitative real-time PCR and western blot were performed to analyze the expressions of osteogenesis, osteoclast formation and matrix remodeling related genes and proteins. DFC–bone marrow cell co-cultures, osteogenic differentiation and transwell matrigel invasion assay were used to determine the effect of IL-1α on DFCs further. Phosphorylation of JNK, ERK, p65 and p38 were evaluated in the IL-1α-treated group. Specific inhibitors of signal pathways were added to confirm their roles in this process. Results IL-1α decreased the expression of osteogenesis-related genes and proteins, and increased the expression of MMP9 and ratios of RANKL/OPG. DFCs cultured with IL-1α showed weaker osteogenic capacity, higher osteoclastogenic and invasive ability. Moreover, phosphorylation of JNK and p38 were up-regulated and pretreatment with specific inhibitors reversed the effect of IL-1α on DFCs. Conclusions IL-1α suppresses osteogenic differentiation, promotes osteoclastogenic ability and matrix remodeling of DFCs by activating JNK and p38 MAPK signaling pathways.

WNT5A supports viability of senescent human dental follicle cells

2018 ◽  
Vol 455 (1-2) ◽  
pp. 21-28 ◽  
Author(s):  
Christian Morsczeck ◽  
Anja Reck ◽  
Torsten E. Reichert
Keyword(s):  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells

scholarly journals Microarray Analysis of Human Dental Follicle Cells in Osteogenic Differentiation

2009 ◽  
Vol 18 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Haruna Aonuma ◽  
Naomi Ogura ◽  
Yoshikazu Kamino ◽  
Ko Ito ◽  
Toshirou Kondoh
Keyword(s):  
Osteogenic Differentiation ◽  
Microarray Analysis ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells

Comparison of human dental follicle cells (DFCs) and stem cells from human exfoliated deciduous teeth (SHED) after neural differentiation in vitro

2009 ◽  
Vol 14 (4) ◽  
pp. 433-440 ◽  
Author(s):  
Christian Morsczeck ◽  
Florian Völlner ◽  
Michael Saugspier ◽  
Caroline Brandl ◽  
Torsten Eugen Reichert ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Differentiation ◽  
Follicle Cells ◽  
Deciduous Teeth ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Human Exfoliated Deciduous Teeth

scholarly journals Comparison of Odontogenic Differentiation of Human Dental Follicle Cells and Human Dental Papilla Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e62332 ◽  
Author(s):  
Lijuan Guo ◽  
Jie Li ◽  
Xiangchen Qiao ◽  
Mei Yu ◽  
Wei Tang ◽  
...  
Keyword(s):  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Dental Papilla ◽  
Odontogenic Differentiation ◽  
Dental Papilla Cells

scholarly journals Human dental follicle cells express embryonic, mesenchymal and neural stem cells markers

2017 ◽  
Vol 73 ◽  
pp. 121-128 ◽  
Author(s):  
Rodrigo Lopes Lima ◽  
Rosenilde Carvalho Holanda-Afonso ◽  
Vivaldo Moura-Neto ◽  
Ana Maria Bolognese ◽  
Marcos Fabio DosSantos ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells
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