scholarly journals Normothermic Machine Perfusion Combined with Bone Marrow Mesenchymal Stem Cells Improves the Oxidative Stress Response and Mitochondrial Function in Rat Donation After Circulatory Death Livers

2020 ◽  
Vol 29 (13) ◽  
pp. 835-852 ◽  
Author(s):  
Liu Yang ◽  
Huan Cao ◽  
Dong Sun ◽  
Ling Lin ◽  
Wei-Ping Zheng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Oxidative Stress Response ◽  
Machine Perfusion ◽  
Donation After Circulatory Death ◽  
Marrow Mesenchymal Stem Cells ◽  
Normothermic Machine Perfusion ◽  
Circulatory Death

scholarly journals Bone marrow mesenchymal stem cells improve thymus and spleen function of aging rats through affecting P21/PCNA and suppressing oxidative stress

Aging ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 11386-11397
Author(s):  
Zhihong Wang ◽  
Yun Lin ◽  
Shang Jin ◽  
Tiannan Wei ◽  
Zhihai Zheng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Aging Rats ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Conditioned Medium from Bone Marrow Mesenchymal Stem Cells Restored Oxidative Stress-Related Impaired Osteogenic Differentiation

2021 ◽  
Vol 22 (24) ◽  
pp. 13458
Author(s):  
Ragda Saleem ◽  
Samih Mohamed-Ahmed ◽  
Rammah Elnour ◽  
Ellen Berggreen ◽  
Kamal Mustafa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Marrow Mesenchymal Stem Cells ◽  
Antioxidant Proteins

Oxidative stress from high levels of intracellular reactive oxygen species (ROS) has been linked to various bone diseases. Previous studies indicate that mesenchymal stem cells (MSC) secrete bioactive factors (conditioned medium (MSC-CM)) that have antioxidant effects. However, the antioxidant role of MSC-CM on osteogenesis has not been fully studied. We aimed to identify antioxidant proteins in MSC-CM using mass spectrometry-based proteomics and to explore their effects on osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSC) exposed to oxidative stress induced by hydrogen peroxide (H2O2). Our analysis revealed that MSC-CM is comprised of antioxidant proteins that are involved in several biological processes, including negative regulation of apoptosis and positive regulation of cell proliferation. Then, hBMSC exposed to H2O2 were treated with MSC-CM, and the effects on their osteogenic differentiation were evaluated. MSC-CM restored H2O2-induced damage to hBMSC by increasing the antioxidant enzyme-SOD production and the mRNA expression level of the anti-apoptotic BCL-2. A decrease in ROS production and cellular apoptosis was also shown. MSC-CM also modulated mRNA expression levels of osteogenesis-related genes, runt-related transcription factor 2, collagen type I, bone morphogenic protein 2, and osteopontin. Furthermore, collagen type I protein secretion, alkaline phosphatase activity, and in vitro mineralization were increased. These results indicate that MSC-CM contains several proteins with antioxidant and anti-apoptotic properties that restored the impaired hBMSC osteogenic differentiation associated with oxidative stress.

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