Effect of Preconditioned Mesenchymal Stromal Cells on Early Microvascular Disturbance in a Mouse Sepsis Model

2019 ◽  
Vol 28 (24) ◽  
pp. 1595-1606 ◽  
Author(s):  
Nathalie Baudry ◽  
Julie Starck ◽  
Clotilde Aussel ◽  
Kyle Lund ◽  
Marc Aletti ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Sepsis Model ◽  
Mouse Sepsis Model

Protective Therapy with Mitochondria Isolated from Mesenchymal Stromal Cells in a Sepsis Model

Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S78-S79
Author(s):  
F.F. Cruz ◽  
L.P. de Carvalho ◽  
L.L. de Castro ◽  
S.C. Abreu ◽  
J.B. Vieira ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Sepsis Model

scholarly journals Stromal Cell-Derived Factor-1 Enhances the Therapeutic Effects of Human Endometrial Regenerative Cells in a Mouse Sepsis Model

2020 ◽  
Vol 2020 ◽  
pp. 1-14 ◽  
Author(s):  
Wang Jin ◽  
Yiming Zhao ◽  
Yonghao Hu ◽  
Dingding Yu ◽  
Xiang Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stromal Cells ◽  
Tissue Damage ◽  
Stromal Cell ◽  
Therapeutic Effects ◽  
Sepsis Model ◽  
Endometrial Regenerative Cells ◽  
Regenerative Cells ◽  
Stromal Cell Derived Factor ◽  
Mouse Sepsis Model

Endometrial regenerative cells (ERCs) are mesenchymal-like stromal cells obtained from human menstrual blood, whose positive therapeutic effects have been validated in several experimental models. Stromal cell-derived factor-1 (SDF-1), the ligand for CXCR4, plays an important role in the migration of mesenchymal stromal cells. The purpose of this study was to investigate the role of the SDF-1/CXCR4 pathway in the therapeutic effects of ERCs in a mouse sepsis model. Through preexperiment and confirmation, wild-type C57BL/6 mice were intraperitoneally injected with 10 mg/kg lipopolysaccharide (LPS). The therapeutic effects of ERCs with different pretreatments were evaluated by assessing sepsis-related symptoms, detecting tissue damage and measuring levels of inflammatory and oxidative stress-related factors. The in vitro experiments demonstrated that there was a much higher CXCR4 expression on ERCs when they were cocultured with SDF-1. The ex vivo experiment results showed that SDF-1 expression significantly increased in mouse tissues. Further experiments also confirmed that, compared with the unmodified ERC treatment group, SDF-1 pretreatment significantly enhanced the therapeutic effects of ERCs on alleviating sepsis symptoms, ameliorating pathological changes, reducing Bax level, and increasing Bcl-2 and PCNA expressions in mouse liver tissues. Furthermore, it was also found that SDF-1-pretreated ERCs contributed to reducing the levels of proinflammatory cytokines (TNF-α, IL-1β) and increasing the levels of anti-inflammatory factors (IL-4, IL10) in mouse serum, liver, and lung. Moreover, SDF-1-pretreated ERCs could also significantly decrease the levels of iNOS and MDA and increase the expression of Nrf2, HO-1, and SOD in liver tissues. Taken together, these results indicate that SDF-1 pretreatment plays a key role in improving the therapeutic effects of ERCs in alleviating sepsis-related symptoms, reducing tissue damage, regulating inflammatory imbalance, and relieving oxidative stress in a mouse sepsis model, which provides more possibilities for the clinical application of ERCs in sepsis and relevant diseases.

Mechanisms of action and osteogenic activity of bone marrow mesenchymal stromal cells are donor dependent

2014 ◽  
Author(s):  
Julie Leotot ◽  
Angelique Lebouvier ◽  
Philippe Hernigou ◽  
Helene Rouard ◽  
Nathalie Chevallier
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Mechanisms Of Action ◽  
Osteogenic Activity

Modified Mesenchymal Stromal Cells Promote Multiple Metabolic Benefits in Obese Diabetic Mice

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 287-OR
Author(s):  
CLEYTON C. DOMINGUES ◽  
NABANITA KUNDU ◽  
YANA KROPOTOVA ◽  
NEEKI AHMADI ◽  
SABYASACHI SEN
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Diabetic Mice
Sign in / Sign up

Export Citation Format

Share Document