scholarly journals Stirred Suspension Bioreactor Culture of Porcine Induced Pluripotent Stem Cells

2019 ◽  
Vol 28 (18) ◽  
pp. 1264-1275 ◽  
Author(s):  
Kyle Burrell ◽  
Rkia Dardari ◽  
Taylor Goldsmith ◽  
Derek Toms ◽  
Daniel A.F. Villagomez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Bioreactor Culture ◽  
Induced Pluripotent ◽  
Suspension Bioreactor

scholarly journals Stirred Suspension Bioreactor Culture of Porcine Induced Pluripotent Stem Cells

2019 ◽  
Author(s):  
Kyle Burrell ◽  
Rkia Dardari ◽  
Taylor Goldsmith ◽  
Derek Toms ◽  
Daniel A.F. Villagomez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Scale Up ◽  
Culture Method ◽  
The Body ◽  
Future Research ◽  
Bioreactor Culture ◽  
Cell Numbers ◽  
Induced Pluripotent

AbstractInduced pluripotent stem cells (iPSCs) are an attractive cell source for regenerative medicine and the development of therapies, as they can proliferate indefinitely under defined conditions and differentiate into any cell type in the body. Large scale expansion of cells is limited in adherent culture, making it difficult to obtain adequate cell numbers for research. It has been previously shown that stirred suspension bioreactors (SSBs) can be used to culture mouse and human stem cells. Pigs are important pre-clinical models for stem cell research. Therefore, this study investigated the use of SSBs as an alternative culture method for the expansion of iPSCs. Using an established porcine iPSC line as well as a new cell line derived and characterized in the current study, we report that porcine iPSCs (piPSCs) can grow in SSB while maintaining characteristics of pluripotency and karyotypic stability similar to cells grown in traditional two-dimensional static culture. This culture method provides a suitable platform for scale up of cell culture to provide adequate cell numbers for future research applications involving porcine induced pluripotent stem cells.

Directed differentiation of mouse-induced pluripotent stem cells generates dopaminergic nerve

2010 ◽  
Vol 34 (8) ◽  
pp. S36-S36
Author(s):  
Ping Duan ◽  
Xuelin Ren ◽  
Wenhai Yan ◽  
Xuefei Han ◽  
Xu Yan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Directed Differentiation ◽  
Induced Pluripotent

Derivation and Characterization of human Induced Pluripotent Stem Cells

Acta Naturae ◽  
2009 ◽  
Vol 1 (2) ◽  
pp. 91-92 ◽  
Author(s):  
M V Shutova ◽  
A N Bogomazova ◽  
M A Lagarkova ◽  
S L Kiselev
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

Derivation of bovine induced pluripotent stem cells by a transposon approach

2014 ◽  
Author(s):  
Talluri Thirumala Rao ◽  
Dharmendra Kumar ◽  
Silke Glage ◽  
Wiebke Garrels ◽  
Katharina Debowski ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent
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