scholarly journals Stress Forces First Lineage Differentiation of Mouse Embryonic Stem Cells; Validation of a High-Throughput Screen for Toxicant Stress

2019 ◽  
Vol 28 (2) ◽  
pp. 101-113 ◽  
Author(s):  
Quanwen Li ◽  
Erica Louden ◽  
Jordan Zhou ◽  
Sascha Drewlo ◽  
Jing Dai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
High Throughput ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
High Throughput Screen ◽  
Lineage Differentiation

scholarly journals Wnt Signaling Regulates the Lineage Differentiation Potential of Mouse Embryonic Stem Cells through Tcf3 Down-Regulation

PLoS Genetics ◽  
2013 ◽  
Vol 9 (5) ◽  
pp. e1003424 ◽  
Author(s):  
Yaser Atlasi ◽  
Rubina Noori ◽  
Claudia Gaspar ◽  
Patrick Franken ◽  
Andrea Sacchetti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Wnt Signaling ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Down Regulation ◽  
Differentiation Potential ◽  
Lineage Differentiation

scholarly journals Hippo-YAP signaling controls lineage differentiation of mouse embryonic stem cells through modulating the formation of super-enhancers

2020 ◽  
Author(s):  
Xiang Sun ◽  
Zhijun Ren ◽  
Yixian Cun ◽  
Cai Zhao ◽  
Xianglin Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Signaling Pathway ◽  
Embryoid Body ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Hippo Signaling ◽  
Rna Seq ◽  
Lineage Differentiation ◽  
Super Enhancer

Abstract Hippo-YAP signaling pathway functions in early lineage differentiation of pluripotent stem cells, but the detailed mechanisms remain elusive. We found that knockout (KO) of Mst1 and Mst2, two key components of the Hippo signaling in mouse embryonic stem cells (ESCs), resulted in a disruption of differentiation into mesendoderm lineage. To further uncover the underlying regulatory mechanisms, we performed a series of ChIP-seq experiments with antibodies against YAP, ESC master transcription factors and some characterized histone modification markers as well as RNA-seq assays using wild type and Mst KO samples at ES and day 4 embryoid body stage respectively. We demonstrate that YAP is preferentially co-localized with super-enhancer (SE) markers such as Nanog, Sox2, Oct4 and H3K27ac in ESCs. The hyper-activation of nuclear YAP in Mst KO ESCs facilitates the binding of Nanog, Sox2 and Oct4 as well as H3K27ac modification at the loci where YAP binds. Moreover, Mst depletion results in novel SE formation and enhanced liquid-liquid phase-separated Med1 condensates on lineage associated genes, leading to the upregulation of these genes and the distortion of ESC differentiation. Our study reveals a novel mechanism on how Hippo-YAP signaling pathway dictates ESC lineage differentiation.

Heterogeneous Mixed-Lineage Differentiation of Mouse Embryonic Stem Cells Induced by Conditioned Media from A549 Cells

2014 ◽  
Vol 23 (16) ◽  
pp. 1923-1936 ◽  
Author(s):  
Shimon Lecht ◽  
Jonathan A. Gerstenhaber ◽  
Collin T. Stabler ◽  
Pimchanok Pimton ◽  
Seda Karamil ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
A549 Cells ◽  
Mouse Embryonic Stem Cells ◽  
Conditioned Media ◽  
Lineage Differentiation

Protein tyrosine phosphatase 1B (PTP1B) is required for cardiac lineage differentiation of mouse embryonic stem cells

2016 ◽  
Vol 425 (1-2) ◽  
pp. 95-102
Author(s):  
Zahra Shokati Eshkiki ◽  
Mohammad Hossein Ghahremani ◽  
Parisa Shabani ◽  
Sattar Gorgani Firuzjaee ◽  
Asie Sadeghi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Protein Tyrosine Phosphatase ◽  
Tyrosine Phosphatase ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Protein Tyrosine Phosphatase 1B ◽  
Protein Tyrosine ◽  
Lineage Differentiation ◽  
Cardiac Lineage

Array‐Based High‐Throughput Screening in Mouse Embryonic Stem Cells with sh RNAs

2013 ◽  
Vol 26 (1) ◽  
Author(s):  
Chia‐Hui Wang ◽  
Nianhan Ma ◽  
Yu‐Tsen Lin ◽  
Cheng‐Chung Wu ◽  
Hong‐Jin Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
High Throughput ◽  
High Throughput Screening ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells

Stepwise renal lineage differentiation of mouse embryonic stem cells tracing in vivo development

2012 ◽  
Vol 417 (2) ◽  
pp. 897-902 ◽  
Author(s):  
Masaki Nishikawa ◽  
Naomi Yanagawa ◽  
Nobuhiko Kojima ◽  
Shunsuke Yuri ◽  
Peter V. Hauser ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Lineage Differentiation
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