scholarly journals Morphological Analysis of Live Undifferentiated Cells Derived from Induced Pluripotent Stem Cells

2018 ◽  
Vol 27 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Yukihiko Osawa ◽  
Tomoyuki Miyamoto ◽  
Setsuyo Ohno ◽  
Eiji Ohno
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Morphological Analysis ◽  
Undifferentiated Cells ◽  
Induced Pluripotent

scholarly journals Morphological Analysis of Human Induced Pluripotent Stem Cells During Induced Differentiation and Reverse Programming

2014 ◽  
Vol 3 (5) ◽  
pp. 206-216 ◽  
Author(s):  
Anne-Marie Courtot ◽  
Aurélie Magniez ◽  
Noufissa Oudrhiri ◽  
Olivier Féraud ◽  
Josette Bacci ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Morphological Analysis ◽  
Induced Differentiation ◽  
Induced Pluripotent

Residual Undifferentiated Cells During Differentiation of Induced Pluripotent Stem Cells In Vitro and In Vivo

2012 ◽  
Vol 21 (4) ◽  
pp. 521-529 ◽  
Author(s):  
Wei Fu ◽  
Shu Jun Wang ◽  
Guang Dong Zhou ◽  
Wei Liu ◽  
Yilin Cao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Undifferentiated Cells ◽  
Induced Pluripotent

scholarly journals Characterization of dystroglycan binding in adhesion of human induced pluripotent stem cells to laminin-511 E8 fragment

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yumika Sugawara ◽  
Keisuke Hamada ◽  
Yuji Yamada ◽  
Jun Kumai ◽  
Motoi Kanagawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Amino Acid ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Coiled Coil ◽  
Integrin Receptors ◽  
Undifferentiated Cells ◽  
Adhesion Activity ◽  
Induced Pluripotent

Abstract Human induced pluripotent stem cells (hiPSCs) grow indefinitely in culture and have the potential to regenerate various tissues. In the development of cell culture systems, a fragment of laminin-511 (LM511-E8) was found to improve the proliferation of stem cells. The adhesion of undifferentiated cells to LM511-E8 is mainly mediated through integrin α6β1. However, the involvement of non-integrin receptors remains unknown in stem cell culture using LM511-E8. Here, we show that dystroglycan (DG) is strongly expressed in hiPSCs. The fully glycosylated DG is functionally active for laminin binding, and although it has been suggested that LM511-E8 lacks DG binding sites, the fragment does weakly bind to DG. We further identified the DG binding sequence in LM511-E8, using synthetic peptides, of which, hE8A5-20 (human laminin α5 2688–2699: KTLPQLLAKLSI) derived from the laminin coiled-coil domain, exhibited DG binding affinity and cell adhesion activity. Deletion and mutation studies show that LLAKLSI is the active core sequence of hE8A5-20, and that, K2696 is a critical amino acid for DG binding. We further demonstrated that hiPSCs adhere to hE8A5-20-conjugated chitosan matrices. The amino acid sequence of DG binding peptides would be useful to design substrata for culture system of undifferentiated and differentiated stem cells.

Directed differentiation of mouse-induced pluripotent stem cells generates dopaminergic nerve

2010 ◽  
Vol 34 (8) ◽  
pp. S36-S36
Author(s):  
Ping Duan ◽  
Xuelin Ren ◽  
Wenhai Yan ◽  
Xuefei Han ◽  
Xu Yan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Directed Differentiation ◽  
Induced Pluripotent

Derivation and Characterization of human Induced Pluripotent Stem Cells

Acta Naturae ◽  
2009 ◽  
Vol 1 (2) ◽  
pp. 91-92 ◽  
Author(s):  
M V Shutova ◽  
A N Bogomazova ◽  
M A Lagarkova ◽  
S L Kiselev
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent
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