Correlation of CDC42 Activity with Cell Proliferation and Palmitate-Mediated Cell Death in Human Umbilical Cord Wharton's Jelly Derived Mesenchymal Stromal Cells

2017 ◽  
Vol 26 (17) ◽  
pp. 1283-1292 ◽  
Author(s):  
Jun Lu ◽  
Qing-Hua Wang ◽  
Liang-Hu Huang ◽  
Hui-Yue Dong ◽  
Ling-Jing Lin ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Death ◽  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Wharton’S Jelly ◽  
Human Umbilical Cord ◽  
Wharton's Jelly

Wharton’s Jelly Mesenchymal Stromal Cells from Human Umbilical Cord: a Close-up on Immunomodulatory Molecules Featured In Situ and In Vitro

2019 ◽  
Vol 15 (6) ◽  
pp. 900-918 ◽  
Author(s):  
Tiziana Corsello ◽  
Giandomenico Amico ◽  
Simona Corrao ◽  
Rita Anzalone ◽  
Francesca Timoneri ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Wharton’S Jelly ◽  
Human Umbilical Cord ◽  
Wharton's Jelly

scholarly journals Mesenchymal stromal cells derived from whole human umbilical cord exhibit similar properties to those derived from Wharton's jelly and bone marrow

FEBS Open Bio ◽  
2016 ◽  
Vol 6 (11) ◽  
pp. 1054-1066 ◽  
Author(s):  
Claire Mennan ◽  
Sharon Brown ◽  
Helen McCarthy ◽  
Eleni Mavrogonatou ◽  
Dimitris Kletsas ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Wharton’S Jelly ◽  
Human Umbilical Cord ◽  
Wharton's Jelly

Stability characteristic of cryopreserved human umbilical cord wharton's jelly–derived mesenchymal stromal cells

Cytotherapy ◽  
2019 ◽  
Vol 21 (5) ◽  
pp. S86
Author(s):  
W. Panta ◽  
H. Kunkanjanawan ◽  
T. Kunkanjanawan ◽  
R. Parnpai ◽  
V. Khemarangsan
Keyword(s):  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Wharton’S Jelly ◽  
Stability Characteristic ◽  
Human Umbilical Cord ◽  
Wharton's Jelly

Mesenchymal stromal cells of human umbilical cord Wharton's jelly accelerate wound healing by paracrine mechanisms

Cytotherapy ◽  
2012 ◽  
Vol 14 (10) ◽  
pp. 1171-1181 ◽  
Author(s):  
Ryutaro Shohara ◽  
Akihito Yamamoto ◽  
Sachiko Takikawa ◽  
Akira Iwase ◽  
Hideharu Hibi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Wharton’S Jelly ◽  
Human Umbilical Cord ◽  
Accelerate Wound Healing ◽  
Wharton's Jelly

scholarly journals Efficient Non-Viral Gene Modification of Mesenchymal Stromal Cells from Umbilical Cord Wharton’s Jelly with Polyethylenimine

Pharmaceutics ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 896
Author(s):  
Ana Isabel Ramos-Murillo ◽  
Elizabeth Rodríguez ◽  
Karl Beltrán ◽  
Cristian Ricaurte ◽  
Bernardo Camacho ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Transfection Efficiency ◽  
Adipogenic Differentiation ◽  
Wharton’S Jelly ◽  
Viral Gene ◽  
Human Umbilical Cord ◽  
Wharton's Jelly ◽  
Significant Difference

Mesenchymal stromal cells (MSC) derived from human umbilical cord Wharton’s jelly (WJ) have a wide therapeutic potential in cell therapy and tissue engineering because of their multipotential capacity, which can be reinforced through gene therapy in order to modulate specific responses. However, reported methodologies to transfect WJ-MSC using cationic polymers are scarce. Here, WJ-MSC were transfected using 25 kDa branched- polyethylenimine (PEI) and a DNA plasmid encoding GFP. PEI/plasmid complexes were characterized to establish the best transfection efficiencies with lowest toxicity. Expression of MSC-related cell surface markers was evaluated. Likewise, immunomodulatory activity and multipotential capacity of transfected WJ-MSC were assessed by CD2/CD3/CD28-activated peripheral blood mononuclear cells (PBMC) cocultures and osteogenic and adipogenic differentiation assays, respectively. An association between cell number, PEI and DNA content, and transfection efficiency was observed. The highest transfection efficiency (15.3 ± 8.6%) at the lowest toxicity was achieved using 2 ng/μL DNA and 3.6 ng/μL PEI with 45,000 WJ-MSC in a 24-well plate format (200 μL). Under these conditions, there was no significant difference between the expression of MSC-identity markers, inhibitory effect on CD3+ T lymphocytes proliferation and osteogenic/adipogenic differentiation ability of transfected WJ-MSC, as compared with non-transfected cells. These results suggest that the functional properties of WJ-MSC were not altered after optimized transfection with PEI.

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