Identification and Characterization of Secondary Neural Tube-Derived Embryonic Neural Stem Cells In Vitro

2015 ◽  
Vol 24 (10) ◽  
pp. 1171-1181 ◽  
Author(s):  
Mohammed R. Shaker ◽  
Joo Yeon Kim ◽  
Hyun Kim ◽  
Woong Sun
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Neural Tube ◽  
Identification And Characterization

In Vivo and in Vitro Characterization of the Angiogenic Effect of CTX0E03 Human Neural Stem Cells

2013 ◽  
Vol 22 (9) ◽  
pp. 1541-1552 ◽  
Author(s):  
Caroline Hicks ◽  
Lara Stevanato ◽  
Robert P. Stroemer ◽  
Ellen Tang ◽  
Sheila Richardson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Human Neural Stem Cells ◽  
In Vitro Characterization ◽  
Angiogenic Effect

scholarly journals Clonal identification and characterization of self-renewing pluripotent stem cells in the developing liver

2002 ◽  
Vol 156 (1) ◽  
pp. 173-184 ◽  
Author(s):  
Atsushi Suzuki ◽  
Yun-wen Zheng ◽  
Shin Kaneko ◽  
Masafumi Onodera ◽  
Katashi Fukao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Duodenal Wall ◽  
Intestinal Epithelial ◽  
Hepatic Stem Cells ◽  
Differentiation Potential ◽  
Endodermal Origin ◽  
Identification And Characterization

Using flow cytometry and single cell–based assays, we prospectively identified hepatic stem cells with multilineage differentiation potential and self-renewing capability. These cells could be clonally propagated in culture where they continuously produced hepatocytes and cholangiocytes as descendants while maintaining primitive stem cells. When cells that expanded in vitro were transplanted into recipient animals, they morphologically and functionally differentiated into hepatocytes and cholangiocytes with reconstitution of hepatocyte and bile duct structures. Furthermore, these cells differentiated into pancreatic ductal and acinar cells or intestinal epithelial cells when transplanted into pancreas or duodenal wall. These data indicate that self-renewing pluripotent stem cells persist in the developing mouse liver and that such cells can be induced to become cells of other organs of endodermal origin under appropriate microenvironment. Manipulation of hepatic stem cells may provide new insight into therapies for diseases of the digestive system.

Neural Stem Cells in Neurospheres, Embryoid Bodies, and Central Nervous System of Human Embryos

2011 ◽  
Vol 17 (4) ◽  
pp. 520-527 ◽  
Author(s):  
A. Henry Sathananthan
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Neural Stem Cells ◽  
Neural Tube ◽  
Embryonic Stem ◽  
Embryoid Bodies ◽  
Surface Epithelium ◽  
Human Embryos

AbstractThe process of neurogenesis and formation of neural stem cells is reported in human neurospheres (NS) and embryoid bodies (EB) derived from human embryonic stem cells, in vitro, and compared with neural tissue formed in human ectopic embryos in week 4 (stage 9), developed in vivo. This morphological study was done using digital imaging by light microscopy and routine transmission electron microscopy. Both NS and EB form neural rosettes from the surface epithelium much like the process of neural tube formation from ectoderm in the embryo. The rosette is the developmental signature of neuroprogenitors in cultures of differentiating embryonic stem cells and is a radial arrangement of columnar cells that express many of the proteins expressed in neuroepithelial cells in the neural tube. The NS produce all of the major classes of progeny of the neural tube, some of which have been documented here. Specific neural markers expressed in the NS and the clinical implications of this study in cell therapy are also discussed.

Characterization of Rat Neural Stem Cells from Embryonic Striatum and Mesencephalon During in Vitro Differentiation

1998 ◽  
pp. 133-147 ◽  
Author(s):  
Eulalia Bazán ◽  
Miguel A. López-Toledano ◽  
Carolina Redondo ◽  
Alberto Alcázar ◽  
Maria A. Mena ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
In Vitro Differentiation

Characterization of neural stem cells in the dorsal vagal complex of adult rat by in vivo proliferation labeling and in vitro neurosphere assay

Neuroscience ◽  
2006 ◽  
Vol 138 (1) ◽  
pp. 5-16 ◽  
Author(s):  
C. Charrier ◽  
V. Coronas ◽  
J. Fombonne ◽  
M. Roger ◽  
A. Jean ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Dorsal Vagal Complex ◽  
Adult Rat ◽  
Neurosphere Assay
Sign in / Sign up

Export Citation Format

Share Document