scholarly journals Stable Methylation at Promoters Distinguishes Epiblast Stem Cells from Embryonic Stem Cells and the In Vivo Epiblasts

2014 ◽  
Vol 23 (17) ◽  
pp. 2014-2029 ◽  
Author(s):  
Anne-Clémence Veillard ◽  
Hendrik Marks ◽  
Andreia Sofia Bernardo ◽  
Luc Jouneau ◽  
Denis Laloë ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Epiblast Stem Cells

scholarly journals Derivation of Mouse Parthenogenetic Advanced Stem Cells

2021 ◽  
Vol 22 (16) ◽  
pp. 8976
Author(s):  
Mengyi Wei ◽  
Jindun Zhang ◽  
Jia Liu ◽  
Chaoyue Zhao ◽  
Shuo Cao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Defined Medium ◽  
Marker Genes ◽  
Developmental Potential ◽  
Epiblast Stem Cells ◽  
Long Time ◽  
Pluripotent Marker

Parthenogenetic embryos have been widely studied as an effective tool related to paternal and maternal imprinting genes and reproductive problems for a long time. In this study, we established a parthenogenetic epiblast-like stem cell line through culturing parthenogenetic diploid blastocysts in a chemically defined medium containing activin A and bFGF named paAFSCs. The paAFSCs expressed pluripotent marker genes and germ-layer-related genes, as well as being alkaline-phosphatase-positive, which is similar to epiblast stem cells (EpiSCs). We previously showed that advanced embryonic stem cells (ASCs) represent hypermethylated naive pluripotent embryonic stem cells (ESCs). Here, we converted paAFSCs to ASCs by replacing bFGF with bone morphogenetic protein 4 (BMP4), CHIR99021, and leukemia inhibitory factor (LIF) in a culture medium, and we obtained parthenogenetic advanced stem cells (paASCs). The paASCs showed similar morphology with ESCs and also displayed a stronger developmental potential than paAFSCs in vivo by producing chimaeras. Our study demonstrates that maternal genes could support parthenogenetic EpiSCs derived from blastocysts and also have the potential to convert primed state paAFSCs to naive state paASCs.

scholarly journals Dickkopf Wnt signaling pathway inhibitor 1 regulates the differentiation of mouse embryonic stem cells in vitro and in vivo

2015 ◽  
Vol 13 (1) ◽  
pp. 720-730 ◽  
Author(s):  
LIPING OU ◽  
LIAOQIONG FANG ◽  
HEJING TANG ◽  
HAI QIAO ◽  
XIAOMEI ZHANG ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Wnt Signaling ◽  
Signaling Pathway ◽  
Wnt Signaling Pathway ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells

scholarly journals Epiblastin A Induces Reprogramming of Epiblast Stem Cells Into Embryonic Stem Cells by Inhibition of Casein Kinase 1

2016 ◽  
Vol 23 (4) ◽  
pp. 494-507 ◽  
Author(s):  
Andrei Ursu ◽  
Damir J. Illich ◽  
Yasushi Takemoto ◽  
Arthur T. Porfetye ◽  
Miao Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Casein Kinase ◽  
Casein Kinase 1 ◽  
Epiblast Stem Cells

10. Embryonic Stem Cells Used for Disc Regeneration in an In Vivo Model of Disc Degeneration

2008 ◽  
Vol 8 (5) ◽  
pp. 5S ◽  
Author(s):  
Ramiro Perez De La Torre ◽  
Hormoz Sheikh ◽  
Mick Perez-Cruet ◽  
Chistopher Fecek ◽  
Rasul Chaudhry ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Disc Degeneration ◽  
Embryonic Stem ◽  
In Vivo Model ◽  
Disc Regeneration
Sign in / Sign up

Export Citation Format

Share Document