The Neural Stem Cell Lineage Reveals Novel Relationships Among Spermatogonial Germ Stem Cells and Other Pluripotent Stem Cells

2014 ◽  
Vol 23 (7) ◽  
pp. 767-778 ◽  
Author(s):  
Anouk-Martine Teichert ◽  
Schreiber Pereira ◽  
Brenda Coles ◽  
Radha Chaddah ◽  
Susan Runciman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Pluripotent Stem Cells ◽  
Cell Lineage ◽  
Stem Cell Lineage

mSEL-1L deficiency affects vasculogenesis and neural stem cell lineage commitment

2017 ◽  
Vol 233 (4) ◽  
pp. 3152-3163 ◽  
Author(s):  
Marina Cardano ◽  
Giuseppe R. Diaferia ◽  
Luciano Conti ◽  
Simona Baronchelli ◽  
Alessandro Sessa ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Lineage ◽  
Lineage Commitment ◽  
Stem Cell Lineage

scholarly journals Cortical Neural Stem Cell Lineage Progression Is Regulated by Extrinsic Signaling Molecule Sonic Hedgehog

Cell Reports ◽  
2020 ◽  
Vol 30 (13) ◽  
pp. 4490-4504.e4 ◽  
Author(s):  
Yue Zhang ◽  
Guoping Liu ◽  
Teng Guo ◽  
Xiaoyi G. Liang ◽  
Heng Du ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Sonic Hedgehog ◽  
Cell Lineage ◽  
Signaling Molecule ◽  
Stem Cell Lineage

Bcr-Abl Impairs T Cell Development From Murine Induced Pluripotent Stem Cells and Hematopoietic Stem Cells; A Partial Explanation for the Concept That Ph+ Clone Never Involves T Cell Lineage in CML,

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 3748-3748
Author(s):  
Bidisha Chanda ◽  
Kiyoko Izawa ◽  
Ratanakanit Harnprasopwat ◽  
Keisuke Takahashi ◽  
Seiichiro Kobayashi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
T Cell ◽  
Hematopoietic Stem Cells ◽  
Pluripotent Stem Cells ◽  
Conflicts Of Interest ◽  
T Cell Development ◽  
Cell Lineage ◽  
Cell Development ◽  
Hematopoietic Stem

Abstract Abstract 3748 Chronic myeloid leukemia (CML) is a clonal myeloproliferative disorder generally believed to originate from a hematopoietic stem cell carrying the BCR-ABL fusion gene, which generally encodes 210kD and 190kD constitutively active tyrosine kinases termed as p210 and p190, respectively. In spite of the putative stem cell origin and the competence for differentiation toward mature B cells, there is a longstanding consensus that CML never involves the T cell lineage at least in chronic phase. To gain insight into this apparent conflict, we used in vitro T cell differentiation model from murine pluripotent stem cells (PSCs) as well as hematopoietic stem cells (HSCs). C57BL/6 MEFs were reprogrammed using a polycistronic lentiviral Tet-On vector encoding human Oct4, Sox2 and Klf4, which were tandemly linked via porcine teschovirus-1 2A peptides, together with another lentiviral vector expressing rtTA driven by the EF-1a promoter. Almost all the vector sequences including the transgenes were deleted by adenovirus-mediated transduction of Crerecombinase after derivation of iPSCs, and only remnant 291-bp LTRs containing a single loxP site remained in the genome. A clone of MEF-iPSCs were retrovirally transduced with p190DccER, a ligand-controllable p190-estrogen receptor fusion protein, whose tyrosine kinase activity absolutely depends on 4-hydroxytamoxyfen (4-HT).For T cell lineage differentiation, p190DccER-MEF-iPSCs were recovered from a feeder-free culture supplemented with LIF and plated onto a subconfluent OP9-DL1 monolayer in the presence of Flt3 ligand and IL7 with or without 0.5 mM 4-HT.After 3 weeks of culture, iPSC-derived blood cells were collected and subjected to FACS analysis for their lineage confirmation. About 70% of lymphocyte-like cells from the 4-HT(-) culture expressed CD3, but only 20% of counterparts from the 4-HT(+)culture expressed CD3, suggesting impaired T cell development by Bcr-Abl. Next, c-Kit+Sca1+Lin− (KSL) bone marrow cells were prepared by FACS from 8-weeks old C57BL/6 mice treated with 5-FU. KSL cells were similarly transduced with p190DccER and were subjected to the OP9-DL1co-culture system with or without 0.5 mM 4-HT.After 2 weeks of culture, 90% of lymphocytes from the 4-HT(-)culture revealed CD3+TCRβ+ phenotype, but only 30% of those were double positive in the presence of 4-HT(+). In addition, 96% of lymphocytes from the 4-HT(-) culture progressed to the DN2 stage with c-Kit−CD44+CD25+phenotype, whereas 40% of those from the 4-HT(+) culture arrested at the DN1 stage showing c-Kit+CD44+CD25−.Since IL7 plays a central role at the stage from DN1 to DN2 of progenitor T cells, Bcr-Abl is suggested to impair T cell development possibly through interfering with the IL7 signal. The precise mechanism underlying impaired T lymphopoiesis by Bcr-Abl is under investigation. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Single-Cell Transcriptomic Analysis Defines Heterogeneity and Transcriptional Dynamics in the Adult Neural Stem Cell Lineage

Cell Reports ◽  
2017 ◽  
Vol 18 (3) ◽  
pp. 777-790 ◽  
Author(s):  
Ben W. Dulken ◽  
Dena S. Leeman ◽  
Stéphane C. Boutet ◽  
Katja Hebestreit ◽  
Anne Brunet
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Neural Stem Cell ◽  
Cell Lineage ◽  
Transcriptomic Analysis ◽  
Adult Neural Stem Cell ◽  
Transcriptional Dynamics ◽  
Stem Cell Lineage

scholarly journals The Spectrin-Actin-Based Periodic Cytoskeleton as a Conserved Nanoscale Scaffold and Ruler of the Neural Stem Cell Lineage

Cell Reports ◽  
2018 ◽  
Vol 24 (6) ◽  
pp. 1512-1522 ◽  
Author(s):  
Meghan Hauser ◽  
Rui Yan ◽  
Wan Li ◽  
Nicole A. Repina ◽  
David V. Schaffer ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Lineage ◽  
Stem Cell Lineage

scholarly journals Neural stem cell lineage-specific cannabinoid type-1 receptor regulates neurogenesis and plasticity in the adult mouse hippocampus

2018 ◽  
Vol 28 (12) ◽  
pp. 4454-4471 ◽  
Author(s):  
Tina Zimmermann ◽  
Mattia Maroso ◽  
Annika Beer ◽  
Sarah Baddenhausen ◽  
Susann Ludewig ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Adult Mouse ◽  
Cell Lineage ◽  
Cannabinoid Type 1 Receptor ◽  
Stem Cell Lineage ◽  
Mouse Hippocampus

scholarly journals Data defining markers of human neural stem cell lineage potential

Data in Brief ◽  
2016 ◽  
Vol 7 ◽  
pp. 206-215 ◽  
Author(s):  
Lotta E. Oikari ◽  
Rachel K. Okolicsanyi ◽  
Lyn R. Griffiths ◽  
Larisa M. Haupt
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Lineage ◽  
Human Neural Stem Cell ◽  
Stem Cell Lineage
Sign in / Sign up

Export Citation Format

Share Document