scholarly journals Influence of Activin A Supplementation During Human Embryonic Stem Cell Derivation on Germ Cell Differentiation Potential

2013 ◽  
Vol 22 (23) ◽  
pp. 3141-3155 ◽  
Author(s):  
Galbha Duggal ◽  
Björn Heindryckx ◽  
Sharat Warrier ◽  
Thomas O'Leary ◽  
Margot Van der Jeught ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Germ Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Activin A ◽  
Differentiation Potential ◽  
Germ Cell Differentiation ◽  
Stem Cell Derivation

Lamin A/C expression is a marker of mouse and human embryonic stem cell differentiation

Stem Cells ◽  
2006 ◽  
Vol 24 (2) ◽  
pp. 474-474 ◽  
Author(s):  
Dan Constantinescu ◽  
Heather L. Gray ◽  
Paul J. Sammak ◽  
Gerald P. Schatten ◽  
Antonei B. Csoka
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Lamin A ◽  
Embryonic Stem Cell Differentiation

scholarly journals Dynamic Proteomic Analysis of Pancreatic Mesenchyme Reveals Novel Factors That Enhance Human Embryonic Stem Cell to Pancreatic Cell Differentiation

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Holger A. Russ ◽  
Limor Landsman ◽  
Christopher L. Moss ◽  
Roger Higdon ◽  
Renee L. Greer ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Beta Cell ◽  
Embryonic Stem Cell ◽  
Proteomic Analysis ◽  
Human Embryonic Stem Cell ◽  
Pancreatic Beta Cell ◽  
Embryonic Stem ◽  
Pancreatic Cell

Current approaches in human embryonic stem cell (hESC) to pancreatic beta cell differentiation have largely been based on knowledge gained from developmental studies of the epithelial pancreas, while the potential roles of other supporting tissue compartments have not been fully explored. One such tissue is the pancreatic mesenchyme that supports epithelial organogenesis throughout embryogenesis. We hypothesized that detailed characterization of the pancreatic mesenchyme might result in the identification of novel factors not used in current differentiation protocols. Supplementing existing hESC differentiation conditions with such factors might create a more comprehensive simulation of normal development in cell culture. To validate our hypothesis, we took advantage of a novel transgenic mouse model to isolate the pancreatic mesenchyme at distinct embryonic and postnatal stages for subsequent proteomic analysis. Refined sample preparation and analysis conditions across four embryonic and prenatal time points resulted in the identification of 21,498 peptides with high-confidence mapping to 1,502 proteins. Expression analysis of pancreata confirmed the presence of three potentially important factors in cell differentiation: Galectin-1 (LGALS1), Neuroplastin (NPTN), and the Lamininα-2 subunit (LAMA2). Two of the three factors (LGALS1 and LAMA2) increased expression of pancreatic progenitor transcript levels in a published hESC to beta cell differentiation protocol. In addition, LAMA2 partially blocks cell culture induced beta cell dedifferentiation. Summarily, we provide evidence that proteomic analysis of supporting tissues such as the pancreatic mesenchyme allows for the identification of potentially important factors guiding hESC to pancreas differentiation.

scholarly journals Combinatorial Extracellular Matrices for Human Embryonic Stem Cell Differentiation in 3D

2010 ◽  
Vol 11 (8) ◽  
pp. 1909-1914 ◽  
Author(s):  
Fan Yang ◽  
Seung-Woo Cho ◽  
Sun Mi Son ◽  
Sarah P. Hudson ◽  
Said Bogatyrev ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Extracellular Matrices ◽  
Embryonic Stem Cell Differentiation

The Influence of Early Embryo Traits on Human Embryonic Stem Cell Derivation Efficiency

2011 ◽  
Vol 20 (5) ◽  
pp. 785-793 ◽  
Author(s):  
Thomas O'Leary ◽  
Björn Heindryckx ◽  
Sylvie Lierman ◽  
Margot Van der Jeught ◽  
Björn Menten ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Early Embryo ◽  
Stem Cell Derivation

scholarly journals Analysis of Mitochondrial Function and Localisation during Human Embryonic Stem Cell Differentiation In Vitro

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e52214 ◽  
Author(s):  
Andrew B. J. Prowse ◽  
Fenny Chong ◽  
David A. Elliott ◽  
Andrew G. Elefanty ◽  
Edouard G. Stanley ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cell ◽  
Mitochondrial Function ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation

scholarly journals Exogenous supplementation of Activin A enhances germ cell differentiation of human embryonic stem cells†

2015 ◽  
Vol 21 (5) ◽  
pp. 410-423 ◽  
Author(s):  
Galbha Duggal ◽  
Björn Heindryckx ◽  
Sharat Warrier ◽  
Jasin Taelman ◽  
Margot Van der Jeught ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Differentiation ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Activin A ◽  
Germ Cell Differentiation
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