scholarly journals Activin A Promotes Hematopoietic Fated Mesoderm Development Through Upregulation of Brachyury in Human Embryonic Stem Cells

2012 ◽  
Vol 21 (15) ◽  
pp. 2866-2877 ◽  
Author(s):  
Chantal Cerdan ◽  
Brendan A.S. McIntyre ◽  
Rami Mechael ◽  
Marilyne Levadoux-Martin ◽  
Jiabi Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Activin A ◽  
Mesoderm Development

scholarly journals Replication Stress and Telomere Dysfunction Are Present in Cultured Human Embryonic Stem Cells

2015 ◽  
Vol 146 (4) ◽  
pp. 251-260 ◽  
Author(s):  
Christine Janson ◽  
Kristine Nyhan ◽  
John P. Murnane
Keyword(s):  
Stem Cells ◽  
Dna Damage ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Chromosome Instability ◽  
Embryonic Stem ◽  
Replication Stress ◽  
Activin A ◽  
Fragile Sites ◽  
Telomere Dysfunction

Replication stress causes DNA damage at fragile sites in the genome. DNA damage at telomeres can initiate breakage-fusion-bridge cycles and chromosome instability, which can result in replicative senescence or tumor formation. Little is known about the extent of replication stress or telomere dysfunction in human embryonic stem cells (hESCs). hESCs are grown in culture with the expectation of being used therapeutically in humans, making it important to minimize the levels of replication stress and telomere dysfunction. Here, the hESC line UCSF4 was cultured in a defined medium with growth factor Activin A, exogenous nucleosides, or DNA polymerase inhibitor aphidicolin. We used quantitative fluorescence in situ hybridization to analyze individual telomeres for dysfunction and observed that it can be increased by aphidicolin or Activin A. In contrast, adding exogenous nucleosides relieved dysfunction, suggesting that telomere dysfunction results from replication stress. Whether these findings can be applied to other hESC lines remains to be determined. However, because the loss of telomeres can lead to chromosome instability and cancer, we conclude that hESCs grown in culture for future therapeutic purposes should be routinely checked for replication stress and telomere dysfunction.

Instant Hepatic Differentiation of Human Embryonic Stem Cells Using Activin a and a Deleted Variant of HGF

2006 ◽  
Vol 15 (10) ◽  
pp. 865-871 ◽  
Author(s):  
Yong Chen ◽  
Alejandro Soto-Gutierrez ◽  
Nalu Navarro-Alvarez ◽  
Jorge David Rivas-Carrillo ◽  
Tomoki Yamatsuji ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Activin A ◽  
Hepatic Differentiation

scholarly journals Exogenous supplementation of Activin A enhances germ cell differentiation of human embryonic stem cells†

2015 ◽  
Vol 21 (5) ◽  
pp. 410-423 ◽  
Author(s):  
Galbha Duggal ◽  
Björn Heindryckx ◽  
Sharat Warrier ◽  
Jasin Taelman ◽  
Margot Van der Jeught ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Differentiation ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Activin A ◽  
Germ Cell Differentiation
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