Elutriated Stem Cells Derived from the Adult Bone Marrow Differentiate into Insulin-Producing Cells In Vivo and Reverse Chemical Diabetes

2012 ◽  
Vol 21 (1) ◽  
pp. 86-96 ◽  
Author(s):  
Svetlana Iskovich ◽  
Nitza Goldenberg-Cohen ◽  
Jerry Stein ◽  
Isaac Yaniv ◽  
Ina Fabian ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Chemical Diabetes ◽  
Adult Bone Marrow ◽  
Insulin Producing Cells ◽  
Adult Bone

In vivo differentiation of stem cells in the aorta-gonad-mesonephros region of mouse embryo and adult bone marrow

2002 ◽  
Vol 30 (8) ◽  
pp. 957-966 ◽  
Author(s):  
Hiroshi Tamura ◽  
Shiki Okamoto ◽  
Ken Iwatsuki ◽  
Yoshiki Futamata ◽  
Kiyoko Tanaka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mouse Embryo ◽  
Adult Bone Marrow ◽  
In Vivo Differentiation ◽  
Adult Bone

In vivo chondrogenesis of adult bone-marrow-derived autologous mesenchymal stem cells

2005 ◽  
Vol 319 (3) ◽  
pp. 429-438 ◽  
Author(s):  
Jinwu Chen ◽  
Changyong Wang ◽  
Shuanghong L� ◽  
Junzheng Wu ◽  
Ximin Guo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adult Bone Marrow ◽  
Autologous Mesenchymal Stem Cells ◽  
Adult Bone

scholarly journals Correction: In Vivo Tumorigenesis Was Observed after Injection of In Vitro Expanded Neural Crest Stem Cells Isolated from Adult Bone Marrow

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0256477
Author(s):  
Sabine Wislet-Gendebien ◽  
Christophe Poulet ◽  
Virginie Neirinckx ◽  
Benoit Hennuy ◽  
James T. Swingland ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Neural Crest ◽  
Neural Crest Stem Cells ◽  
Adult Bone Marrow ◽  
Adult Bone

Differential Expression of Alpha2 Integrin In Human Bone Marrow and Cord Blood CD34+CD38- Progenitors and Stem Cells Reconstituting NOD/SCID-IL2Rgammacnull Mice.

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 2609-2609
Author(s):  
Wan Man Wong ◽  
Ingbritt Åstrand-Grundström ◽  
Hong Qian ◽  
Marja Ekblom
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cord Blood ◽  
Adult Bone Marrow ◽  
Cord Blood Cd34 ◽  
Alpha2 Receptor ◽  
Cell Fractions ◽  
Adult Bone

Abstract Abstract 2609 Human primitive stem cells reside in the CD34+CD38- fraction in cord blood and bone marrow. However, there is a high level of heterogeneity in these cell fractions, and the phenotype of the rare primitive stem cells remains poorly defined. We have studied expression of integrin alpha2 chain, a member of a family of beta1 integrin cell adhesion receptors, in CD34+CD38- cells in adult bone marrow and cord blood. >90% of bone marrow CD34+CD38- cells and >95% of CD34+CD38-CD90+ cells, enriched in long-term in vivo reconstituting stem cells (Majeti et al., Cell Stem Cell 1:635, 2007) expressed the integrin alpha2 chain. In contrast, in cord blood CD34+CD38- and the CD34+CD38-CD90+ subpopulation, the integrin alpha2 chain was expressed only in 37.1+/− 5.3% and 32.2+/− 4.9% of the cells (mean+/− SD), respectively. To determine whether integrin alpha2 expression could be used to identify functionally distinct stem and progenitor cell populations in cord blood and bone marrow, we isolated CD34+CD38- integrin alpha2+ and alpha2- cells by flow cytometry and analyzed these by in vivo transplantation into immunodeficient NOD/SCID-IL2Rgammacnull (NSG) mice and by in vitro progenitor cell assays (long-term culture initiating cell, LTC-IC, and colony assays). Transplantation of cord blood CD34+CD38- integrin alpha2+ cells resulted in significantly higher level of human CD45+ (p<0.05), myeloid (p<0.01) and CD34+ (p<0.05) cell engraftment at 16–18 weeks after transplantation than integrin alpha2- cells (reconstitution/300 cells in age and sex matched recipients, Figure 1). In contrast, there were no differences in reconstitution at 12 weeks in mice transplanted with cord blood CD34+CD38- alpha2+ and alpha2- cells. Because of lower engraftment capacity of adult bone marrow cells in immunodeficient mice, bone marrow CD34+CD38- cells were analyzed after intra-bone transplantation. After 12 weeks only few mice transplanted with the CD34+CD38- integrin alpha2+ cells but none transplanted with the corresponding alpha2- cells were reconstituted with human CD45+ or myeloid cells at a level '0.1%. The LTC-IC progenitors within the CD34+CD38- populations, assayed after 6 weeks culture on stroma, in adult bone marrow were highly enriched in the alpha2+ as compared with alpha2- fraction (mean +/− SD 96.7+/− 57.0 and 0.2+/− 0.6 colonies/50 cells, respectively), whereas in cord blood they resided equally in both integrin alpha2+ and alpha2- cell fractions (mean +/− SD 77.4+/− 59.4 and 108.8+/− 96.0 colonies/50 cells, respectively). The lineage committed CFU-GM and BFU-E progenitors in adult bone marrow were within integrin CD34+CD38- alpha2+ and alpha2- fractions (mean +/− SD 8.5+/− 5.3 and 14.9+/− 16.2 colonies/100 cells, respectively), whereas in cord blood they were significantly enriched in the alpha2- fraction (mean +/− SD 9.3+/− 5.5 and 22.0+/− 6.7 colonies/100 cells in alpha2+ and alpha2- fractions, respectively). Taken together, our results show expression of integrin alpha2 receptor in most of the primitive cord blood long-term in vivo reconstituting stem cells, with a gradual loss of the integrin alpha2 receptor during maturation to short term in vivo reconstituting stem cells, LTC-IC and lineage committed progenitors. Furthermore, our findings show distinct ontogeny-related differences in the expression of the integrin alpha2 receptor in the functionally defined primitive and lineage-committed CD34+CD38- progenitors, indicating differences in cellular interactions of cord blood and bone marrow progenitors with the hematopoietic niches. Disclosures: Ekblom: Bristol-Myers Squibb: Honoraria.

scholarly journals In Vivo Tumorigenesis Was Observed after Injection of In Vitro Expanded Neural Crest Stem Cells Isolated from Adult Bone Marrow

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e46425 ◽  
Author(s):  
Sabine Wislet-Gendebien ◽  
Christophe Poulet ◽  
Virginie Neirinckx ◽  
Benoit Hennuy ◽  
James T. Swingland ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Neural Crest ◽  
Neural Crest Stem Cells ◽  
Adult Bone Marrow ◽  
Adult Bone

Adult bone marrow-derived stem cells use R-cadherin to target sites of neovascularization in the developing retina

Blood ◽  
2004 ◽  
Vol 103 (9) ◽  
pp. 3420-3427 ◽  
Author(s):  
Michael I. Dorrell ◽  
Atsushi Otani ◽  
Edith Aguilar ◽  
Stacey K. Moreno ◽  
Martin Friedlander
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Vascular Endothelial Cells ◽  
Retinal Vasculature ◽  
Hematopoietic Stem ◽  
Adult Bone Marrow ◽  
Calcium Dependent ◽  
Dependent Cell ◽  
Adult Bone

Abstract Adult bone marrow contains a population of hematopoietic stem cells (HSCs) that can give rise to cells capable of targeting sites of neovascularization in the peripheral or retinal vasculature. However, relatively little is known about the mechanism of targeting of these cells to sites of neovascularization. We have analyzed subpopulations of HSCs for the expression of a variety of cell surface adhesion molecules and found that R-cadherin, a calcium-dependent cell-cell adhesion molecule important for normal retinal endothelial cell guidance, was preferentially expressed by functionally targeting HSCs. Preincubation of HSCs with function-blocking anti-R-cadherin antibodies or novel R-cadherin-specific peptide antagonists effectively prevented targeting of bone marrow-derived cells to the developing retinal vasculature in vivo. Whereas control-injected HSCs targeted to all 3 normal developing retinal vascular layers, blocking R-cadherin-mediated adhesion resulted in mistargeting of the HSCs to the normally avascular outer retina. Our results suggest that vascular targeting of bone marrow-derived HSCs is dependent on mechanisms similar to those used by endogenous retinal vascular endothelial cells. Thus, R-cadherin antagonists may be useful in the treatment of neovascular diseases in which circulating HSCs contribute to abnormal angiogenesis. (Blood. 2004;103:3420-3427)

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