Physiological Properties of Neurons Derived from Human Embryonic Stem Cells Using a Dibutyryl Cyclic AMP-Based Protocol

2011 ◽  
Vol 20 (10) ◽  
pp. 1733-1746 ◽  
Author(s):  
Glenn S. Belinsky ◽  
Anna R. Moore ◽  
Shaina M. Short ◽  
Matthew T. Rich ◽  
Srdjan D. Antic
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cyclic Amp ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Dibutyryl Cyclic Amp ◽  
Physiological Properties

scholarly journals Essentiality of CTNNB1 in Malignant Transformation of Human Embryonic Stem Cells under Long-Term Suboptimal Conditions

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Jie Liu ◽  
Sicong Zeng ◽  
Yang Wang ◽  
Juan Yu ◽  
Qi Ouyang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Malignant Transformation ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Continuous Exposure ◽  
Aberrant Expression ◽  
Physiological Properties ◽  
Potential Biomarker

Human embryonic stem cells (hESCs) gradually accumulate abnormal karyotypes during long-term suboptimal culture, which hinder their application in regenerative medicine. Previous studies demonstrated that the activation of CTNNB1 might be implicated in this process. Hence, the hESC line with stably silenced CTNNB1 was established to further explore the role of CTNNB1 in the malignant transformation of hESCs. It was shown to play a vital role in the maintenance of the physiological properties of stem cells, such as proliferation, migration, differentiation, and telomere regulation. Furthermore, the malignant transformation of hESCs was induced by continuous exposure to 0.001 μg/ml mitomycin C (MMC). The results showed that CTNNB1 and its target genes, including proto-oncogenes CCND1 and C-MYC, were aberrantly upregulated in hESCs after MMC treatment. Moreover, the high expression of CTNNB1 accelerated cell transition from G0/G1 phase to the S phase and stimulated the growth of cells containing breakage-fusion-bridge (BFB) cycles. Conversely, CTNNB1 silencing inhibited these effects and triggered a survival crisis. The current data indicated that CTNNB1 is intimately associated with the physiological properties of stem cells; however, the aberrant expression of CTNNB1 is involved in the malignant transformation of hESCs, which might advance the process by facilitating telomere-related unstable cell proliferation. Thus, the aberrant CTNNB1 level might serve as a potential biomarker for detecting the malignant transformation of hESCs.

Ethanol alters cell cycle gene expression in human embryonic stem cells

2016 ◽  
Vol 01 (03) ◽  
pp. 201-208 ◽  
Author(s):  
Malini Krishnamoorthy ◽  
Brian Gerwe ◽  
Jamie Heimburg-Molinaro ◽  
Rachel Nash ◽  
Jagan Arumugham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Cycle Gene ◽  
Cell Cycle Gene Expression
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