scholarly journals Endothelial Cells from Embryonic Stem Cells in a Chemically Defined Medium

2011 ◽  
Vol 20 (12) ◽  
pp. 2153-2161 ◽  
Author(s):  
Alicia A. Blancas ◽  
Albert J. Shih ◽  
Nicholas E. Lauer ◽  
Kara E. McCloskey
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Defined Medium ◽  
Chemically Defined Medium

Neural differentiation of mouse embryonic stem cells in chemically defined medium

2005 ◽  
Vol 68 (1-2) ◽  
pp. 62-75 ◽  
Author(s):  
Isabelle A. Bouhon ◽  
Hidemasa Kato ◽  
Sidharthan Chandran ◽  
Nicholas D. Allen
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Defined Medium ◽  
Mouse Embryonic Stem Cells ◽  
Chemically Defined Medium

Chemically defined medium supporting cardiomyocyte differentiation of human embryonic stem cells

2008 ◽  
Vol 76 (9) ◽  
pp. 958-970 ◽  
Author(s):  
Xiu Qin Xu ◽  
Ralph Graichen ◽  
Set Yen Soo ◽  
Thavamalar Balakrishnan ◽  
Siti Norfiza Bte Rahmat ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Cardiomyocyte Differentiation

scholarly journals Differentiation of Human Embryonic Stem Cells to Regional Specific Neural Precursors in Chemically Defined Medium Conditions

PLoS ONE ◽  
2008 ◽  
Vol 3 (5) ◽  
pp. e2122 ◽  
Author(s):  
Slaven Erceg ◽  
Sergio Laínez ◽  
Mohammad Ronaghi ◽  
Petra Stojkovic ◽  
Maria Amparo Pérez-Aragó ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Neural Precursors

scholarly journals OVOL2 is a critical regulator of ER71/ETV2 in generating FLK1+, hematopoietic, and endothelial cells from embryonic stem cells

Blood ◽  
2014 ◽  
Vol 124 (19) ◽  
pp. 2948-2952 ◽  
Author(s):  
Ju Young Kim ◽  
Ra Ham Lee ◽  
Tae Min Kim ◽  
Dong-Wook Kim ◽  
Young-Joo Jeon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Embryonic Stem Cells ◽  
Binding Protein ◽  
Embryonic Stem ◽  
Erythroid Cells ◽  
Key Points

Key Points OVOL2 is identified as a novel binding protein of ER71. Interaction between ER71 and OVOL2 cooperatively regulates the generation of FLK1+ mesoderm, and endothelial and erythroid cells.

scholarly journals Endothelial cells derived from embryonic stem cells respond to cues from topographical surface patterns

2013 ◽  
Vol 7 (1) ◽  
pp. 18 ◽  
Author(s):  
Rachel Hatano ◽  
Kevin Mercurio ◽  
Jesus Luna ◽  
Drew E Glaser ◽  
Valerie J Leppert ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Surface Patterns

scholarly journals Wnt signaling promotes hematoendothelial cell development from human embryonic stem cells

Blood ◽  
2008 ◽  
Vol 111 (1) ◽  
pp. 122-131 ◽  
Author(s):  
Petter S. Woll ◽  
Julie K. Morris ◽  
Matt S. Painschab ◽  
Rebecca K. Marcus ◽  
Aimee D. Kohn ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Embryonic Stem Cells ◽  
Wnt Signaling ◽  
Human Embryonic Stem Cells ◽  
Stromal Cells ◽  
Hematopoietic Cells ◽  
Embryonic Stem ◽  
Canonical Wnt Signaling ◽  
Canonical Wnt

Human embryonic stem cells (hESCs) provide an important means to effectively study soluble and cell-bound mediators that regulate development of early blood and endothelial cells in a human model system. Here, several complementary methods are used to demonstrate canonical Wnt signaling is important for development of hESC-derived cells with both hematopoietic and endothelial potential. Analyses using both standard flow cy-tometry, as well the more detailed high-throughput image scanning flow cytometry, characterizes sequential development of distinct early developing CD34brightCD31+Flk1+ cells and a later population of CD34dimCD45+ cells. While the CD34brightCD31+Flk1+ have a more complex morphology and can develop into both endothelial cells and hematopoietic cells, the CD34dimCD45+ cells have a simpler morphology and give rise to only hematopoietic cells. Treatment with dickkopf1 to inhibit Wnt signaling results in a dramatic decrease in development of cells with hematoendothelial potential. In addition, activation of the canonical Wnt signaling pathway in hESCs by coculture with stromal cells that express Wnt1, but not use of noncanonical Wnt5-expressing stromal cells, results in an accelerated differentiation and higher percentage of CD34brightCD31+Flk1+ cells at earlier stages of differentiation. These studies effectively demonstrate the importance of canonical Wnt signaling to mediate development of early hematoendothelial progenitors during human development.

scholarly journals Neuroprotective Effects of Mesenchymal Stem Cells Derived from Human Embryonic Stem Cells in Transient Focal Cerebral Ischemia in Rats

2009 ◽  
Vol 29 (4) ◽  
pp. 780-791 ◽  
Author(s):  
Yi-Ping Liu ◽  
Hakan Seçkin ◽  
Yusuf İzci ◽  
Zhong Wei Du ◽  
Yi-Ping Yan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Mesenchymal Stem Cells ◽  
Embryonic Stem Cells ◽  
Cerebral Ischemia ◽  
Human Embryonic Stem Cells ◽  
Focal Cerebral Ischemia ◽  
Embryonic Stem ◽  
Transient Focal Cerebral Ischemia ◽  
Infarction Volume

Embryonic mesenchymal stem cells (eMSCs) were first derived from human embryonic stem cells (hESCs) overexpressing green fluorescence protein (GFP). They expressed CD29, CD44, CD73, CD105, CD166 and nestin, but not CD34, CD45, CD106 SSEA-4 or Oct3/4. Twenty million eMSCs in 1mL of phosphate-buffered saline (PBS) were injected into the femoral veins of spontaneously hypertensive rats after transient middle cerebral artery occlusion. The migration and differentiation of the eMSCs in the ischemic brain were analyzed. The results revealed that eMSCs migrated to the infarction region and differentiated into neurons, which were positive for β-tubulin III, microtubule-associated protein 2 (MAP2), HuC, neurofilament and human nuclear antibody, and to vascular endothelial cells, which were positive for von Willebrand factor (vWF). The transplanted cells survived in the infarction region for at least 4 weeks. Adhesive removal function significantly improved in the first week after cell transplantation, and rotarod motor function significantly improved starting from the second week. The infarction volume in the eMSC group was significantly smaller than that in the PBS control group at 4 weeks after infusion. The results of this study show that when administered intravenously, eMSCs differentiated into neuronal and endothelial cells, reduced the infarction volume, and improved behavioral functional outcome significantly in transient focal cerebral ischemia.

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