scholarly journals Successful Periodontal Ligament Regeneration by Periodontal Progenitor Preseeding on Natural Tooth Root Surfaces

2011 ◽  
Vol 20 (10) ◽  
pp. 1659-1668 ◽  
Author(s):  
Smit Jayant Dangaria ◽  
Yoshihiro Ito ◽  
Xianghong Luan ◽  
Thomas G.H. Diekwisch
Keyword(s):  
Periodontal Ligament ◽  
Tooth Root ◽  
Root Surfaces ◽  
Natural Tooth

scholarly journals Biomaterial-Based Approaches for Regeneration of Periodontal Ligament and Cementum Using 3D Platforms

2019 ◽  
Vol 20 (18) ◽  
pp. 4364 ◽  
Author(s):  
Chan Ho Park
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Periodontal Ligament ◽  
State Of The Art ◽  
Tooth Root ◽  
Tissue Formation ◽  
Periodontal Tissue ◽  
Periodontal Tissues ◽  
Root Surfaces ◽  
Periodontal Ligaments

Currently, various tissue engineering strategies have been developed for multiple tissue regeneration and integrative structure formations as well as single tissue formation in musculoskeletal complexes. In particular, the regeneration of periodontal tissues or tooth-supportive structures is still challenging to spatiotemporally compartmentalize PCL (poly-ε-caprolactone)-cementum constructs with micron-scaled interfaces, integrative tissue (or cementum) formations with optimal dimensions along the tooth-root surfaces, and specific orientations of engineered periodontal ligaments (PDLs). Here, we discuss current advanced approaches to spatiotemporally control PDL orientations with specific angulations and to regenerate cementum layers on the tooth-root surfaces with Sharpey’s fiber anchorages for state-of-the-art periodontal tissue engineering.

An in vitro model for adhesion of bacteria to human tooth root surfaces

1994 ◽  
Vol 39 (2) ◽  
pp. 155-161 ◽  
Author(s):  
Lech M. Switalski ◽  
Wade G. Butcher
Keyword(s):  
In Vitro Model ◽  
Human Tooth ◽  
Tooth Root ◽  
Vitro Model ◽  
Root Surfaces

scholarly journals Molecular Markers of Early Orthodontic Tooth Movement

2009 ◽  
Vol 79 (6) ◽  
pp. 1108-1113 ◽  
Author(s):  
Patricia Joyce Brooks ◽  
Dorrin Nilforoushan ◽  
Morris Frank Manolson ◽  
Craig A. Simmons ◽  
Siew-Ging Gong
Keyword(s):  
Periodontal Ligament ◽  
Tooth Movement ◽  
Early Stage ◽  
Expression Patterns ◽  
Orthodontic Tooth Movement ◽  
Contralateral Side ◽  
Tooth Root ◽  
Ki 67 ◽  
Related Transcription Factor ◽  
Osteoclastic Differentiation

Abstract Objective: To understand the molecular basis of early orthodontic tooth movement by looking at the expression of KI-67, runt-related transcription factor 2 (Runx2), and tumor necrosis factor ligand superfamily member 11 (RANKL) proteins. Materials and Methods: We employed a rat model of early orthodontic tooth movement using a split-mouth design (where contralateral side serves as a control) and performed immunohistochemical staining to map the spatial expression patterns of three proteins at 3 and 24 hours after appliance insertion. Results: We observed increased expression of KI-67, a proliferation marker, and RANKL, a molecule associated with osteoclastic differentiation, in the compression sites of the periodontal ligament subjected to 3 hours of force. In contrast, there was increased expression of KI-67 and Runx2, a marker of osteoblast precursors, in tension areas after 24 hours of force. Decreased KI-67 expression in the mesial and distal regions of the periodontal ligament was observed at the midpoint of the tooth root. Conclusions: The early RANKL expression indicates that at this early stage cells are involved in osteoclast precursor signaling. Also, decreased KI-67 expression found near the midpoint of the tooth root is believed to represent the center of rotation, providing a molecular means of visualizing mechanical loading patterns.

The Attachment of V79 and Human Periodontal Ligament Fibroblasts on Periodontally Involved Root Surfaces Following Treatment with EDTA, Citric Acid, or Tetracycline HCL: An SEM in vitro Study

2006 ◽  
Vol 7 (1) ◽  
pp. 35-43 ◽  
Author(s):  
R. Viswa Chandra ◽  
Ganesh Chandra Jagetia ◽  
K. Mahalinga Bhat
Keyword(s):  
Citric Acid ◽  
Periodontal Ligament ◽  
Chinese Hamster ◽  
Lung Fibroblasts ◽  
Control Group ◽  
Periodontal Ligament Fibroblasts ◽  
Human Periodontal Ligament Fibroblasts ◽  
Root Surfaces ◽  
Tetracycline Hcl

Abstract Objective The present in vitro study has been designed to establish and compare the effects of citric acid, EDTA, and tetracycline HCl on human periodontally diseased roots on the structure, attachment, and orientation of V79 (primary Chinese hamster lung fibroblasts) cells and human periodontal ligament fibroblasts (HPDL). Materials and Methods Commercially availableV79 cells and HPDL derived from healthy human third molars were used in this study. These fibroblasts were left in solution for seven days in order to attain confluence. Forty single-rooted teeth were obtained from patients diagnosed with periodontitis. The crown part was removed under constant irrigation and the root was split vertically into two equal halves, thus, yielding 80 specimens. Following scaling and root planing, the specimens were washed with phosphate buffered saline (PBS) and kept in 50 μg/ml gentamycin sulphate solution for 24 hours. The root pieces were then treated as follows: citric acid at pH 1, 24% EDTA, or with a 10% solution of tetracycline HCl and were then placed in V79 fibroblast cultures and HPDL cultures. The specimens were harvested after four weeks and were fixed in 2.5% glutaraldehyde in PBS before preparation for scanning electron microscopy (SEM). Results The behavior of V79 cells was similar to that of human periodontal ligament cells on root conditioned surfaces. V79 and HPDL showed a healthy morphology on root surfaces treated with citric acid and EDTA and a relatively unhealthy appearance on root surfaces treated with tetracycline HCl and distilled water (control group). Conclusion The results suggest the use of citric acid and EDTA as root conditioning agents favorably affects the migration, attachment, and morphology of fibroblasts on human root surfaces, which may play a significant role in periodontal healing and regeneration. Citation Chandra RV, Jagetia GC, Bhat KM. The Attachment of V79 and Human Periodontal Ligament Fibroblasts on Periodontally Involved Root Surfaces Following Treatment with EDTA, Citric Acid, or Tetracycline HCL: An SEM in vitro Study. J Contemp Dent Pract 2006 February;(7)1:044-059.

Human periodontal ligament fibroblast response to rhPDGF-BB application on periodontally diseased root surfaces-in vitro

2013 ◽  
Vol 31 (4) ◽  
pp. 130-138 ◽  
Author(s):  
Kamedh Yashwant Chowdhary ◽  
Joann Pauline George ◽  
Purushothama Gowda ◽  
Jyothsana A. Rao
Keyword(s):  
Periodontal Ligament ◽  
Human Periodontal Ligament Fibroblast ◽  
Diseased Root ◽  
Periodontal Ligament Fibroblast ◽  
Root Surfaces
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