Impact of Zinc Fingers and Homeoboxes 3 on the Regulation of Mesenchymal Stem Cell Osteogenic Differentiation

2011 ◽  
Vol 20 (9) ◽  
pp. 1539-1547 ◽  
Author(s):  
Fumio Suehiro ◽  
Masahiro Nishimura ◽  
Takeshi Kawamoto ◽  
Masami Kanawa ◽  
Yuu Yoshizawa ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Zinc Fingers

miR-143 Inhibits Osteogenic Differentiation of Umbilical Cord Mesenchymal Stem Cell by Targeting Zinc Fingers and Homeoboxes 3

2019 ◽  
Vol 9 (11) ◽  
pp. 1550-1556
Author(s):  
Haiying Chen ◽  
Lei Zhang ◽  
Lijun Tao
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Western Blot ◽  
Osteogenic Differentiation ◽  
Umbilical Cord ◽  
Osteoblast Differentiation ◽  
Bioinformatics Analysis ◽  
Zinc Fingers ◽  
Alizarin Red ◽  
Msc Differentiation

The transcription regulating factor-zinc fingers and homeoboxes 3 (ZHX3) involve in osteoblast differentiation. miR-143 regulates osteoblast differentiation. Bioinformatics analysis revealed a relationship between miR-143 and the 3′ -UTR of ZHX3. This study assessed miR-143's role in UC-MSC differentiation. The UC-MSCs were induced to differentiate into osteoblasts. The expressions of miR-143, ZHX3, RUNX2, Osteopontin (OPN) and Osteocalcin (OC) genes were detected by qRTPCR on days 0, 20 and 30. The UC-MSCs cells were divided into agomir-NC group and miR-143 agomir group. After 30 days of differentiation, the expression of ZHX3, RUNX2, OPN and OC protein was detected by western blot. During differentiation of UC-MSCs into osteoblasts, miR-143 expression was decreased, ZHX3 expression, ALP activity and alizarin red staining was increased. There was a targeted regulation relationship between miR-143 and ZHX3. Transfection of agomir143 significantly downregulated ZHX3, RUNX2, OPN, OC genes and proteins, significantly reduced alizarin red staining, and decreased the ability of osteoblast differentiation. Decreased miR-143 expression plays a role in up-regulating ZHX3 expression and promoting osteoblast differentiation of UC-MSCs. Overexpression of miR-143 can inhibit UC-MSCs differentiation into osteoblasts by targeting ZHX3.

scholarly journals Melatonin promotes bone marrow mesenchymal stem cell osteogenic differentiation and prevents osteoporosis development through modulating circ_0003865 that sponges miR-3653-3p

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Xudong Wang ◽  
Taiqiu Chen ◽  
Zhihuai Deng ◽  
Wenjie Gao ◽  
Tongzhou Liang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Luciferase Reporter ◽  
Specific Gene ◽  
Biological Processes ◽  
Alizarin Red ◽  
Qrt Pcr ◽  
Dual Luciferase Reporter Assay

Abstract Background Little is known about the implications of circRNAs in the effects of melatonin (MEL) on bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation and osteoporosis (OP) progression. The aim of our study was to investigate circRNAs in MEL-regulated BMSC differentiation and OP progression. Methods BMSC osteogenic differentiation was measured by qRT-PCR, western blot (WB), Alizarin Red, and alkaline phosphatase (ALP) staining. Differential circRNA and mRNA profiles of BMSCs treated by MEL were characterized by deep sequencing, followed by validation using RT-PCR, Sanger sequencing, and qRT-PCR. Silencing and overexpression of circ_0003865 were conducted for functional investigations. The sponged microRNAs and targeted mRNAs were predicted by bioinformatics and validated by qRT-PCR, RNA pull-down, and dual-luciferase reporter assay. The function of miR-3653-3p and circ_0003865/miR-3653-3p/growth arrest-specific gene 1 (GAS1) cascade was validated for the osteogenic differentiation of BMSCs by CCK-8, qRT-PCR, WB, Alizarin Red, and ALP staining. The effects of circ_0003865 on OP development were tested in murine OP model. Results MEL promoted osteogenic differentiation of BMSCs. RNA sequencing revealed significant alterations in circRNA and mRNA profiles associated with multiple biological processes and signaling pathways. Circ_0003865 expression in BMSCs was significantly decreased by MEL treatment. Silencing of circ_0003865 had no effect on proliferation while promoted osteogenic differentiation of BMSCs. Overexpression of circ_0003865 abrogated the promotion of BMSC osteogenic differentiation induced by MEL, but proliferation of BMSCs induced by MEL had no change whether circ_0003865 was overexpression or not. Furthermore, circ_0003865 sponged miR-3653-3p to promote GAS1 expression in BMSCs. BMSC osteogenic differentiation was enhanced by miR-3653-3p overexpression while BMSC proliferation was not affected. By contrast, miR-3653-3p silencing mitigated the promoted BMSC osteogenic differentiation caused by circ_0003865 silencing, but had no effect on proliferation. Finally, circ_0003865 silencing repressed OP development in mouse model. Conclusion MEL promotes BMSC osteogenic differentiation and inhibits OP pathogenesis by suppressing the expression of circ_0003865, which regulates GAS1 gene expression via sponging miR-3653-3p.

scholarly journals Mesenchymal stem cell-derived exosomes have altered microRNA profiles and induce osteogenic differentiation depending on the stage of differentiation

PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0193059 ◽  
Author(s):  
Xiaoqin Wang ◽  
Omar Omar ◽  
Forugh Vazirisani ◽  
Peter Thomsen ◽  
Karin Ekström
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation

Functions of the Mg–HA coating on carbon/carbon composite surface to promote the proliferation and osteogenic differentiation of mBMSCs

RSC Advances ◽  
2016 ◽  
Vol 6 (107) ◽  
pp. 105056-105062 ◽  
Author(s):  
Xiong Xinbo ◽  
Ni Xinye ◽  
Zhou Dong
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Carbon Composite ◽  
Composite Surface ◽  
Ha Coating

This study aims to evaluate the functions of the Mg–hydroxyapatite (Mg–HA) bio-coating on carbon/carbon composite (C/C) surface to promote the proliferation and osteogenic differentiation of bone mesenchymal stem cell (BMSCs).

Regulation of the mesenchymal stem cell fate by interleukin-17: Implications in osteogenic differentiation

2021 ◽  
Vol 13 (11) ◽  
pp. 1699-1716
Author(s):  
Jelena Krstić ◽  
Slavko Mojsilović ◽  
Sonja S Mojsilović ◽  
Juan F Santibanez
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Cell Fate ◽  
Interleukin 17 ◽  
Stem Cell Fate

scholarly journals Role of geometrical cues in bone marrow-derived mesenchymal stem cell survival, growth and osteogenic differentiation

2017 ◽  
Vol 32 (7) ◽  
pp. 906-919 ◽  
Author(s):  
Dhanak Gupta ◽  
David M Grant ◽  
Kazi M Zakir Hossain ◽  
Ifty Ahmed ◽  
Virginie Sottile
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Survival ◽  
Osteogenic Differentiation ◽  
Stem Cell Survival
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