Shear Stress Induces Nitric Oxide–Mediated Vascular Endothelial Growth Factor Production in Human Adipose Tissue Mesenchymal Stem Cells

2010 ◽  
Vol 19 (3) ◽  
pp. 371-378 ◽  
Author(s):  
Vinícius Bassaneze ◽  
Valério Garrone Barauna ◽  
Carolina Lavini-Ramos ◽  
Jorge Kalil ◽  
Isolmar Tadeu Schettert ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Stem Cells ◽  
Vascular Endothelial Growth Factor ◽  
Shear Stress ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Vascular Endothelial ◽  
Growth Factor Production ◽  
Endothelial Growth Factor

scholarly journals Mesenchymal Stem Cells Enhance Vascular Endothelial Growth Factor-A, Endothelial Nitric Oxide Synthetase, and HSP70 Expression in Improving Erectile Dysfunction in Streptozotocin-induced Diabetic Rats

2021 ◽  
Vol 9 (A) ◽  
pp. 1174-1180
Author(s):  
Ade Indra Mukti ◽  
Syafruddin Ilyas ◽  
Syah Mirsya Warli ◽  
Agung Putra ◽  
Nur Rasyid ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Stem Cells ◽  
Vascular Endothelial Growth Factor ◽  
Mesenchymal Stem Cells ◽  
Hsp70 Expression ◽  
Vascular Endothelial ◽  
Sham Control ◽  
Nitric Oxide Synthetase ◽  
Endothelial Growth Factor ◽  
Endothelial Nitric Oxide

This study investigated the therapeutic role of mesenchymal stem cells (MSCs) on erectile function in a diabetes mellitus erectile dysfunction (DMED) rat model by analyzing the expression of endothelial nitric oxide synthetase (eNOS), vascular endothelial growth factor (VEGF), and the 70 kilodalton heat shock proteins (HSP70). MSCs were isolated from umbilical cords (UCs), and their characteristics identified by flow cytometry and osteogenic differentiation analysis. Thirty 8-week-old rats were divided into four groups: sham, control, T1, and T2. After a 16 h fast, 24 rats were randomly selected and intraperitoneally injected with streptozotocin (STZ) to induce DM. At 8 weeks after STZ injection, rats with DMED were classified into four groups, sham, control group [DMED rats received 500 μL phosphate buffer saline (PBS)]; T1 [DMED rats treated with 500 μL PBS containing 1 × 106 UC-MSCs]; T2 [DMED rats treated with 500 μL PBS containing 2 × 106 UC-MSCs]. Eight weeks after MSCs administration, the rats’ erectile function was measured by cavernous nerve stimulation. The blinded histological and gene expression assessment were used to analyze the eNOS, HSP70 content, and VEGF expression on the penile tissues. MSCs administration, rats in T1 and T2 groups showed a significant enhancement of erectile response that showed a trend of increase of VEGF mRNA level expression was 2.2 ± 0.61 in T2 Group supported with the optimum recovery of eNOS, in which the value of eNOS expression was 20.66% ± 2.32%. While optimum decrease of HSP70 content, the value of HSP70 expression was 15.50% ± 0.90%. IHC results showed that the DMED induction in rats caused a significant decrease of eNOS content in corpus cavernosum tissue. MSCs could ameliorate DMED in rats by increasing VEGF and decreasing HSP70 and eNOS, indicating these cells offer a potential application for DMED patients’ treatment.

Shear Stress Induces VEGF Production Mediated By Nitric Oxide In Human Adipose Tissue Mesenchymal Stem Cells

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Vinicius Bassaneze ◽  
Ayumi Aurea Miyakawa ◽  
Giancarlo Fatobene ◽  
Isolmar Tadeu Schettert ◽  
Jose Eduardo Krieger
Keyword(s):  
Nitric Oxide ◽  
Stem Cells ◽  
Shear Stress ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Human Adipose Tissue
Sign in / Sign up

Export Citation Format

Share Document