Restricted Spontaneous In Vitro Differentiation and Region-Specific Migration of Long-Term Expanded Fetal Human Neural Precursor Cells After Transplantation Into the Adult Rat Brain

2009 ◽  
Vol 18 (7) ◽  
pp. 1043-1058 ◽  
Author(s):  
Jaroslaw Maciaczyk ◽  
Ilyas Singec ◽  
Donata Maciaczyk ◽  
Alexander Klein ◽  
Guido Nikkhah
Keyword(s):  
Rat Brain ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
In Vitro Differentiation ◽  
Adult Rat ◽  
Adult Rat Brain

In vitro differentiation of human bone marrow stromal cells into neural precursor cells using small molecules

2021 ◽  
Vol 363 ◽  
pp. 109340
Author(s):  
Abeer Sallam ◽  
Thangirala Sudha ◽  
Noureldien H.E. Darwish ◽  
Samar Eghotny ◽  
Abeer E-Dief ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Small Molecules ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Precursor Cells ◽  
Marrow Stromal Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
In Vitro Differentiation

scholarly journals Characterization of Porcine Ventral Mesencephalic Precursor Cells following Long-Term Propagation in 3D Culture

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Pia S. Jensen ◽  
Lise Lyck ◽  
Pia Jensen ◽  
Jens Zimmer ◽  
Morten Meyer
Keyword(s):  
Growth Factor ◽  
Dopaminergic Neurons ◽  
Neural Tissue ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Large Animal ◽  
Ventral Mesencephalic

The potential use of predifferentiated neural precursor cells for treatment of a neurological disorder like Parkinson’s disease combines stem cell research with previous experimental and clinical transplantation of developing dopaminergic neurons. One current obstacle is, however, the lack of ability to generate dopaminergic neurons after long-termin vitropropagation of the cells. The domestic pig is considered a useful nonprimate large animal model in neuroscience, because of a better resemblance of the larger gyrencephalic pig brain to the human brain than the commonly used brains of smaller rodents. In the present study, porcine embryonic (28–30 days), ventral mesencephalic precursor cells were isolated and propagated as free-floating neural tissue spheres in medium containing epidermal growth factor and fibroblast growth factor 2. For passaging, the tissue spheres were cut into quarters, avoiding mechanical or enzymatic dissociation in order to minimize cellular trauma and preserve intercellular contacts. Spheres were propagated for up to 237 days with analysis of cellular content and differentiation at various time points. Our study provides the first demonstration that porcine ventral mesencephalic precursor cells can be long-term propagated as neural tissue spheres, thereby providing an experimental 3Din vitromodel for studies of neural precursor cells, their niche, and differentiation capacity.

scholarly journals Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Giulia Nato ◽  
Alessandro Corti ◽  
Elena Parmigiani ◽  
Elena Jachetti ◽  
Daniele Lecis ◽  
...  
Keyword(s):  
Immune Tolerance ◽  
Prenatal Development ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Neural Precursors ◽  
Adult Rat ◽  
Induced Pluripotent ◽  
Species Specific

AbstractWe xeno-transplanted human neural precursor cells derived from induced pluripotent stem cells into the cerebellum and brainstem of mice and rats during prenatal development or the first postnatal week. The transplants survived and started to differentiate up to 1 month after birth when they were rejected by both species. Extended survival and differentiation of the same cells were obtained only when they were transplanted in NOD-SCID mice. Transplants of human neural precursor cells mixed with the same cells after partial in vitro differentiation or with a cellular extract obtained from adult rat cerebellum increased survival of the xeno-graft beyond one month. These findings are consistent with the hypothesis that the slower pace of differentiation of human neural precursors compared to that of rodents restricts induction of immune-tolerance to human antigens expressed before completion of maturation of the immune system. With further maturation the transplanted neural precursors expressed more mature antigens before the graft were rejected. Supplementation of the immature cells suspensions with more mature antigens may help to induce immune-tolerance for those antigens expressed only later by the engrafted cells.

In vitro differentiation of E-N-CAM expressing rat neural precursor cells isolated by FACS during prenatal development

1994 ◽  
Vol 37 (3) ◽  
pp. 359-373 ◽  
Author(s):  
S. Blass-Kampmann ◽  
S. Reinhardt-Maelicke ◽  
A. Kindler-Röhrborn ◽  
V. Cleeves ◽  
M. F. Rajewsky
Keyword(s):  
Prenatal Development ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
In Vitro Differentiation

In Vitro Metal Inhibition of N-Methyl-D-Aspartate Specific Glutamate Receptor Binding in Neonatal and Adult Rat Brain

1997 ◽  
Vol 20 (1-2) ◽  
pp. 21-29 ◽  
Author(s):  
Bettaiya Rajanna ◽  
Sharada Rajanna ◽  
Elizabeth Hall ◽  
Prabhakara R. Yallapragada
Keyword(s):  
Rat Brain ◽  
Glutamate Receptor ◽  
Receptor Binding ◽  
Adult Rat ◽  
Adult Rat Brain

scholarly journals Diffusion tensor imaging reveals changes in the adult rat brain following long-term and passive moderate acoustic exposure

2016 ◽  
Vol 140 (6) ◽  
pp. 4540-4547 ◽  
Author(s):  
Sherwin Abdoli ◽  
Leon C. Ho ◽  
Jevin W. Zhang ◽  
Celia M. Dong ◽  
Condon Lau ◽  
...  
Keyword(s):  
Diffusion Tensor Imaging ◽  
Rat Brain ◽  
Diffusion Tensor ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Acoustic Exposure
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