Horse Embryonic Stem Cell Lines from the Proliferation of Inner Cell Mass Cells

2006 ◽  
Vol 15 (4) ◽  
pp. 523-531 ◽  
Author(s):  
Xihe Li ◽  
S.G. Zhou ◽  
Marta P. Imreh ◽  
L. Ährlund-Richter ◽  
W.R. Allen
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Stem Cell Lines

scholarly journals Mechanical isolation of the inner cell mass is effective in derivation of new human embryonic stem cell lines

2007 ◽  
Vol 22 (12) ◽  
pp. 3051-3058 ◽  
Author(s):  
S. Strom ◽  
J. Inzunza ◽  
K.-H. Grinnemo ◽  
K. Holmberg ◽  
E. Matilainen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Human Embryonic Stem Cell ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Stem Cell Lines ◽  
Mechanical Isolation

scholarly journals The Ethical Case against Stem Cell Research

2003 ◽  
Vol 12 (4) ◽  
pp. 372-383 ◽  
Author(s):  
SØREN HOLM
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Stem Cell Research ◽  
Human Embryonic Stem Cell ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Ethical Analysis ◽  
Considerable Debate ◽  
Stem Cell Lines

The possibility of creating human embryonic stem cell lines from the inner cell mass of blastocysts has led to considerable debate about how these scientific developments should be regulated. Part of this debate has focused on the ethical analysis and part on how this analysis should influence policymaking.

215 COMPARATIVE ANALYSIS OF PORCINE PLURIPOTENT CELL LINES DERIVED FROM SOMATIC CELLS AND VARIOUS EMBRYONIC ORIGINS

2012 ◽  
Vol 24 (1) ◽  
pp. 220
Author(s):  
J. K. Park ◽  
H. S. Kim ◽  
K. J. Uh ◽  
K. H. Choi ◽  
H. M. Kim ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Pluripotent Cells ◽  
Differentiation Potential

Since pluripotent cells were first derived from the inner cell mass (ICM) of mouse blastocysts, tremendous efforts have been made to establish embryonic stem cell (ESC) lines in several domestic species including the pig; however, authentic porcine ESCs have not yet been established. It has proven difficult to derive pluripotent cells of naïve state that represents full pluripotency, due to the frequent occurrence of spontaneous differentiation into an EpiSC-like state during culture in pigs. We have been able to derive EpiSC-like porcine embryonic stem cell (pESC) lines of a differentiated non-ES cell state from blastocyst stage porcine embryos of various origins, including in vitro fertilized (IVF), in vivo derived, IVF aggregated and parthenogenetic embryos. In addition, we have generated induced pluripotent stem cells (piPSCs) via plasmid transfection of reprogramming factors (Oct4, Sox2, Klf4 and c-Myc) into porcine fibroblast cells. In this study, we analysed characteristics such as marker expression, pluripotency and the X chromosome inactivation (XCI) status of our EpiSC-like pESC lines along with our piPSC line. Our results show that these cell lines demonstrate the expression of genes associated with the Activin/Nodal and FGF2 pathways along with the expression of pluripotent markers Oct4, Sox2, Nanog, SSEA4, TRA 1-60 and TRA 1-81. Furthermore all of these cell lines showed in vitro differentiation potential; female XCI activity and a normal karyotype. Here we provide preliminary results that suggest that, as a nonpermissive species, the porcine species undergoes reprogramming into a primed state during the establishment of pluripotent stem cell lines. This work was supported by the BioGreen 21 Program (#20070401034031, PJ0081382011), Rural Development Administration, Republic of Korea.

Phosphoinositide 3-kinases and regulation of embryonic stem cell fate

2007 ◽  
Vol 35 (2) ◽  
pp. 225-228 ◽  
Author(s):  
M.J. Welham ◽  
M.P. Storm ◽  
E. Kingham ◽  
H.K. Bone
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Fate ◽  
Molecular Mechanisms ◽  
Inner Cell Mass ◽  
Es Cells ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Stem Cell Lines ◽  
Phosphoinositide 3 Kinase

ES (embryonic stem) cell lines are derived from the epiblast of pre-implantation embryos and like the inner cell mass cells from which they are derived exhibit the remarkable property of pluripotency, namely the ability to differentiate into all cell lineages comprising the adult organism. ES cells and their differentiated progeny offer tremendous potential to regenerative medicine, particularly as cellular therapies for the treatment of a wide variety of chronic disorders, such as Type 1 diabetes, Parkinson's disease and retinal degeneration. In order for this potential to be realized, a detailed understanding of the molecular mechanisms regulating the fundamental properties of ES cells, i.e. pluripotency, proliferation and differentiation, is required. In the present paper, we review the evidence that PI3K (phosphoinositide 3-kinase)-dependent signalling plays a role in regulation of both ES cell pluripotency and proliferation.

scholarly journals Generation of two RNF2 homozygous knockout human embryonic stem cell lines by CRISPR/Cas9 system

2020 ◽  
Vol 47 ◽  
pp. 101885
Author(s):  
Yongli Shan ◽  
Lishi Ma ◽  
Cong Zhang ◽  
Yanqi Zhang ◽  
Jingyuan Zhang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Lines

Derivation and characterisation of the human embryonic stem cell lines, NOTT1 and NOTT2

2010 ◽  
Vol 46 (3-4) ◽  
pp. 367-375 ◽  
Author(s):  
Helen Priddle ◽  
Cinzia Allegrucci ◽  
Paul Burridge ◽  
Maria Munoz ◽  
Nigel M. Smith ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Lines
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