Specific and Stable Gene Transfer to Human Embryonic Stem Cells Using Pseudotyped Lentiviral Vectors

2006 ◽  
Vol 15 (1) ◽  
pp. 109-117 ◽  
Author(s):  
Jung-Eun Jang ◽  
Kit Shaw ◽  
Xiao-Jin Yu ◽  
Denise Petersen ◽  
Karen Pepper ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Transfer ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Lentiviral Vectors ◽  
Stable Gene

scholarly journals Nucleofection Mediates High-Efficiency Stable Gene Knockdown and Transgene Expression in Human Embryonic Stem Cells

Stem Cells ◽  
2008 ◽  
Vol 26 (6) ◽  
pp. 1436-1443 ◽  
Author(s):  
Kristi A. Hohenstein ◽  
April D. Pyle ◽  
Jing Yi Chern ◽  
Leslie F. Lock ◽  
Peter J. Donovan
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Transgene Expression ◽  
High Efficiency ◽  
Embryonic Stem ◽  
Gene Knockdown ◽  
Stable Gene

Effective Adenovirus-Mediated Gene Transfer Into Neural Stem Cells Derived From Human Embryonic Stem Cells

2010 ◽  
Vol 19 (4) ◽  
pp. 569-578 ◽  
Author(s):  
Cornelia M. Bertram ◽  
Susan M. Hawes ◽  
Simone Egli ◽  
Swee Lim Peh ◽  
Mirella Dottori ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Transfer ◽  
Neural Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem

scholarly journals Comparison of Gene-Transfer Efficiency in Human Embryonic Stem Cells

2009 ◽  
Vol 12 (1) ◽  
pp. 15-24 ◽  
Author(s):  
Feng Cao ◽  
Xiaoyan Xie ◽  
Timothy Gollan ◽  
Li Zhao ◽  
Kazim Narsinh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Transfer ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Transfer Efficiency ◽  
Gene Transfer Efficiency

scholarly journals PiggyBac Transposon-Mediated, Reversible Gene Transfer in Human Embryonic Stem Cells

2010 ◽  
Vol 19 (6) ◽  
pp. 763-771 ◽  
Author(s):  
You-Tzung Chen ◽  
Kenryo Furushima ◽  
Pei-Shan Hou ◽  
Amy T. Ku ◽  
Jian Min Deng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Transfer ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Piggybac Transposon

scholarly journals Efficient and Stable Transgene Expression in Human Embryonic Stem Cells Using Transposon-Mediated Gene Transfer

Stem Cells ◽  
2007 ◽  
Vol 25 (11) ◽  
pp. 2919-2927 ◽  
Author(s):  
Andrew Wilber ◽  
Jonathan L. Linehan ◽  
Xinghui Tian ◽  
Petter S. Woll ◽  
Julie K. Morris ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Transfer ◽  
Human Embryonic Stem Cells ◽  
Transgene Expression ◽  
Embryonic Stem

Genetic Engineering of Human Embryonic Stem Cells with Lentiviral Vectors

2005 ◽  
Vol 14 (4) ◽  
pp. 367-377 ◽  
Author(s):  
Chen Xiong ◽  
Dong-Qi Tang ◽  
Chang-Qing Xie ◽  
Li Zhang ◽  
Ke-Feng Xu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Genetic Engineering ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Lentiviral Vectors

Improved lentiviral gene transfer into human embryonic stem cells grown in co-culture with murine feeder and stroma cells

2011 ◽  
Vol 392 (10) ◽  
pp. 887-895 ◽  
Author(s):  
Melanie Wurm ◽  
Benjamin Groß ◽  
Malte Sgodda ◽  
Ludger Ständker ◽  
Thomas Müller ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Transfer ◽  
Human Embryonic Stem Cells ◽  
Genetically Modify ◽  
Embryonic Stem ◽  
Cell Types ◽  
Viral Envelope ◽  
Leukaemia Virus ◽  
Transfer Strategies

AbstractGenetic modification of human embryonic stem cells (hESCs) using biophysical DNA transfection methods are hampered by the very low single cell survival rate and cloning efficiency of hESCs. Lentiviral gene transfer strategies are widely used to genetically modify hESCs but limited transduction efficiencies in the presence of feeder or stroma cells present problems, particularly if vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped viral particles are applied. Here, we investigated whether the recently described semen derived enhancer of virus infection (SEVI) and alternative viral envelope proteins derived from either Gibbon ape leukaemia virus (GALV) or feline leukaemia virus (RD114) are applicable for transducing hESCs during co-culture with feeder or stroma cells. Our first set of experiments demonstrates that SEVI has no toxic effect on murine or hESCs and that exposure to SEVI does not interfere with the pluripotency-associated phenotype. Focusing on hESCs, we were able to further demonstrate that SEVI increases the transduction efficiencies of GALV and RD114 pseudotyped lentiviral vectors. More importantly, aiming at targeted differentiation of hESCs into functional somatic cell types, GALV pseudotyped lentiviral particles could efficiently and exclusively transduce hESCs grown in co-culture with OP9-GFP stroma cells (which were often used to induce differentiation into haematopoietic derivatives).

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