Monitoring Early Differentiation Events in Human Embryonic Stem Cells by Massively Parallel Signature Sequencing and Expressed Sequence Tag Scan

2004 ◽  
Vol 13 (6) ◽  
pp. 694-715 ◽  
Author(s):  
Takumi Miura ◽  
Yongquan Luo ◽  
Irina Khrebtukova ◽  
Ralph Brandenberger ◽  
Daixing Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Expressed Sequence Tag ◽  
Massively Parallel Signature Sequencing ◽  
Embryonic Stem ◽  
Massively Parallel ◽  
Early Differentiation ◽  
Parallel Signature Sequencing ◽  
Expressed Sequence

scholarly journals Impact of AHR Ligand TCDD on Human Embryonic Stem Cells and Early Differentiation

2020 ◽  
Vol 21 (23) ◽  
pp. 9052
Author(s):  
Indrek Teino ◽  
Antti Matvere ◽  
Martin Pook ◽  
Inge Varik ◽  
Laura Pajusaar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Target Genes ◽  
Expression Patterns ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Cell Types ◽  
Early Differentiation

Aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor, which mediates the effects of a variety of environmental stimuli in multiple tissues. Recent advances in AHR biology have underlined its importance in cells with high developmental potency, including pluripotent stem cells. Nonetheless, there is little data on AHR expression and its role during the initial stages of stem cell differentiation. The purpose of this study was to investigate the temporal pattern of AHR expression during directed differentiation of human embryonic stem cells (hESC) into neural progenitor, early mesoderm and definitive endoderm cells. Additionally, we investigated the effect of the AHR agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the gene expression profile in hESCs and differentiated cells by RNA-seq, accompanied by identification of AHR binding sites by ChIP-seq and epigenetic landscape analysis by ATAC-seq. We showed that AHR is differentially regulated in distinct lineages. We provided evidence that TCDD alters gene expression patterns in hESCs and during early differentiation. Additionally, we identified novel potential AHR target genes, which expand our understanding on the role of this protein in different cell types.

Connexin 43 is involved in early differentiation of human embryonic stem cells

2019 ◽  
Vol 105 ◽  
pp. 33-44 ◽  
Author(s):  
Qian Peng ◽  
Chaomin Yue ◽  
Andy Chun Hang Chen ◽  
Kai Chuen Lee ◽  
Sze Wan Fong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Connexin 43 ◽  
Embryonic Stem ◽  
Early Differentiation

scholarly journals Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Yoon Young Kim ◽  
Seung-Yup Ku ◽  
Zev Rosenwaks ◽  
Hung Ching Liu ◽  
Sun Kyung Oh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Embryoid Bodies ◽  
Red Ginseng ◽  
Cardiac Progenitors ◽  
Early Differentiation ◽  
Undifferentiated Hescs ◽  
Wide Range

Human embryonic stem cells (hESCs) have capacities to self-renew and differentiate into all cell typesin vitro. Red ginseng (RG) is known to have a wide range of pharmacological effectsin vivo; however, the reports on its effects on hESCs are few. In this paper, we tried to demonstrate the effects of RG on the proliferation and differentiation of hESCs. Undifferentiated hESCs, embryoid bodies (EBs), and hESC-derived cardiac progenitors (CPs) were treated with RG extract at 0.125, 0.25, and 0.5 mg/mL. After treatment of undifferentiated hESCs from day 2 to day 6 of culture, BrdU labeling showed that RG treatment increased the proliferation of hESCs, and the expression of Oct4 and Nanog was increased in RG-treated group. To find out the effects of RG on early differentiation stage cells, EBs were treated with RG extract for 10 days and attached for further differentiation. Immunostaining for three germ layer markers showed that RG treatment increased the expressions of Brachyury and HNF3βon EBs. Also, RG treatment increased the expression of Brachyury in early-stage and of Nkx2.5 in late-stage hESC-derived CPs. These results demonstrate facilitating effects of RG extract on the proliferation and early differentiation of hESC.

scholarly journals Changes in Laminin Expression Pattern during Early Differentiation of Human Embryonic Stem Cells

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0138346 ◽  
Author(s):  
Martin Pook ◽  
Indrek Teino ◽  
Ade Kallas ◽  
Toivo Maimets ◽  
Sulev Ingerpuu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Expression Pattern ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Early Differentiation

scholarly journals Lineage-Specific Early Differentiation of Human Embryonic Stem Cells Requires a G2 Cell Cycle Pause

Stem Cells ◽  
2016 ◽  
Vol 34 (7) ◽  
pp. 1765-1775 ◽  
Author(s):  
Jennifer J. Van Oudenhove ◽  
Rodrigo A. Grandy ◽  
Prachi N. Ghule ◽  
Roxana del Rio ◽  
Jane B. Lian ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Early Differentiation
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