Antimicrobial Photodynamic Therapy of Resistant Bacterial Strains by Indocyanine Green and 809-nm Diode Laser

2013 ◽  
Vol 31 (4) ◽  
pp. 155-162 ◽  
Author(s):  
Nermin Topaloglu ◽  
Murat Gulsoy ◽  
Sahru Yuksel
Keyword(s):  
Photodynamic Therapy ◽  
Indocyanine Green ◽  
Diode Laser ◽  
Antimicrobial Photodynamic Therapy ◽  
Bacterial Strains

Antimicrobial photodynamic therapy of Lactobacillus acidophilus by indocyanine green and 810-nm diode laser

2018 ◽  
Vol 24 ◽  
pp. 145-149 ◽  
Author(s):  
Farzaneh Ahrari ◽  
Mostafa Shahabi ◽  
Reza Fekrazad ◽  
Neda Eslami ◽  
Fatemeh Mazhari ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Indocyanine Green ◽  
Diode Laser ◽  
Lactobacillus Acidophilus ◽  
Antimicrobial Photodynamic Therapy

Evaluation of Antimicrobial Photodynamic Therapy Using Indocyanine Green and Near-Infrared Diode Laser AgainstEnterococcus faecalisin Infected Human Root Canals

2017 ◽  
Vol 35 (5) ◽  
pp. 264-269 ◽  
Author(s):  
Charis Beltes ◽  
Nikolaos Economides ◽  
Hercules Sakkas ◽  
Chrissanthy Papadopoulou ◽  
Theodoros Lambrianidis
Keyword(s):  
Photodynamic Therapy ◽  
Indocyanine Green ◽  
Diode Laser ◽  
Near Infrared ◽  
Antimicrobial Photodynamic Therapy ◽  
Root Canals

scholarly journals Effect of Antimicrobial Photodynamic Therapy Using Indocyanine Green Doped with Chitosan Nanoparticles on Biofilm Formation-Related Gene Expression of Aggregatibacter actinomycetemcomitans

2019 ◽  
Author(s):  
Mehdi Rostami Rad ◽  
Maryam Pourhajibagher ◽  
Amir Reza Reza Rokn ◽  
Hamid Reza Barikani ◽  
Abbas Bahador
Keyword(s):  
Gene Expression ◽  
Photodynamic Therapy ◽  
Indocyanine Green ◽  
Diode Laser ◽  
Virulence Factor ◽  
Biofilm Formation ◽  
Chitosan Nanoparticles ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Expression Level ◽  
Antimicrobial Photodynamic Therapy

Objectives: Eradication of Aggregatibacter actinomycetemcomitans (A. action-mycetemcomitans), as an opportunistic periodontopathogen, and inhibition of its virulence factor expression require a new adjunctive therapeutic method. In this study, we accessed the expression level of rcpA gene, as a virulence factor associated with A. actinomycetemcomitans biofilm formation, following treatment by antimicrobial photodynamic therapy (aPDT) using indocyanine green (ICG) doped with chitosan nanoparticles (CS-NPs@ICG). Materials and Methods: CS-NPs@ICG was synthesized and examined using scanning electron microscopy (SEM). A. actinomycetemcomitans ATCC 33384 strain was treated with CS-NPs@ICG, as a photosensitizer, which was excited with a diode laser at the wavelength of 810 nm with the energy density of 31.2 J/cm2. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to determine the changes in rcpA gene expression level. Results: Synthetized CS-NPs@ICG was confirmed via SEM. The results revealed that CS-NPs@ICG-mediated aPDT could significantly decrease rcpA gene expression to 13.2-fold (P<0.05). There was a remarkable difference between aPDT using CS-NPs@ICG and ICG (P<0.05). The diode laser, ICG, and CS-NPs@ICG were unable to significantly downregulate rcpA gene expression (P>0.05). Conclusion: aPDT with CS-NPs@ICG leads to a decrease of the virulence factor of A. actinomycetemcomitans and can be used as an adjunct to routine treatments for successful periodontal therapy in vivo.

scholarly journals The Effect of Antimicrobial Photodynamic Therapy Using Chlorophyllin–Phycocyanin Mixture on Enterococcus faecalis: The Influence of Different Light Sources

2020 ◽  
Vol 10 (12) ◽  
pp. 4290 ◽  
Author(s):  
Nasim Chiniforush ◽  
Maryam Pourhajibagher ◽  
Steven Parker ◽  
Stefano Benedicenti ◽  
Abbas Bahador ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Diode Laser ◽  
Enterococcus Faecalis ◽  
Control Group ◽  
Human Gingival Fibroblast ◽  
Light Sources ◽  
Gingival Fibroblast ◽  
Antimicrobial Photodynamic Therapy ◽  
Cell Viability Assay ◽  
Vitro Effect

The purpose of this study was to evaluate the in vitro effect of the chlorophyllin–phycocyanin mixture (Photoactive+) as a photosensitizer (PS) during antimicrobial photodynamic therapy (aPDT) on the count of Enterococcus faecalis (E. faecalis) using different light sources. The antimicrobial effect of aPDT with chlorophyllin–phycocyanin mixture using different light sources including diode laser (λ = 660 nm), diode laser (λ = 635 nm), LED (λ = 450 ± 30 nm) alone or in combination was assessed using microbial cell viability assay against E. faecalis. In addition, the cell cytotoxicity of Photoactive+ was assessed on human gingival fibroblast (HuGu) cells by MTT assay; E. faecalis growth when treated by both red wavelengths (635 nm, 660 nm) and combination of LED (420–480 nm) and red wavelengths (635 nm, 660 nm), significantly reduced compared to the control group (p < 0.05). There was no significant reduction in the number of viable cells exposed to Photoactive+ compared to the control group (p < 0.05). This study shows that the application of chlorophyllin–phycocyanin mixture and irradiation with emission of red light achieved a better result for bacterial count reduction, compared to a control. This component can be applied safely due to very negligible cytotoxicity.

scholarly journals Endodontic treatment of a large periapical cyst with the aid of antimicrobial photodynamic therapy - Case report

2019 ◽  
Vol 22 (4) ◽  
pp. 561-568
Author(s):  
Amjad Abu Hasna ◽  
Carlos Henrique Ferrari ◽  
Cláudio Antonio Talge Carvalho
Keyword(s):  
Photodynamic Therapy ◽  
Case Report ◽  
Calcium Hydroxide ◽  
Diode Laser ◽  
Bone Repair ◽  
Irradiation Time ◽  
Radiographic Examination ◽  
Antimicrobial Photodynamic Therapy ◽  
Traditional Treatment ◽  
Digital Palpation

Introduction: Periapical cysts, especially those of great extension, represent a challenge for endodontics, since they are not repaired with traditional treatment. The aim of this case report was to evaluate the effectivity of antimicrobial photodynamic therapy “aPDT” when associated with calcium hydroxide Ca(OH)2 in treating such cysts. Case report:  A 40-year-old female patient complained of bulging in the upper anterior region of the mouth. The clinical examination showed an extensive swollen periodontal area, painful with digital palpation, with a negative response to thermal tests on teeth 11 and 21. After traditional treatment, the canals of 11 and 21 were filled with photosensitizer methylene blue 0.005% pre irradiation time (PIT) of 5 minutes and irradiation by low power diode laser with visible red wavelength (660 nm) during 2 minutes without intervals, using an optical fiber delivering system with a helical movement from apical to cervical. Then the canal were filled with Ca(OH)2 paste for 45 days being changed each 15 days, and repeating the radiation. Results: After 45 days, the patient already had a bulging remission, with painless resistance to apical pressure in the region and signs of bone repair at the radiographic examination, and total absence of signs or symptoms. Conclusion: the association of aPDT with Ca(OH)2 paste is effective in treating of periapical lesions of great extension and avoid surgical treatment.Keywords: Antimicrobial; Calcium hydroxide; Diode Laser; Periapical cysts; Photodynamic Therapy; Root canal treatment.

scholarly journals Broad spectrum antibacterial photodynamic and photothermal therapy achieved with indocyanine green loaded SPIONs under near infrared irradiation

2020 ◽  
Vol 8 (16) ◽  
pp. 4616-4625 ◽  
Author(s):  
K. Bilici ◽  
N. Atac ◽  
A. Muti ◽  
I. Baylam ◽  
O. Dogan ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Indocyanine Green ◽  
Broad Spectrum ◽  
Photothermal Therapy ◽  
Bacterial Infections ◽  
Near Infrared ◽  
Alternative Therapies ◽  
Antimicrobial Photodynamic Therapy ◽  
Infrared Irradiation ◽  
Antibiotic Resistant

Antimicrobial photodynamic therapy (aPDT) and antimicrobial photothermal therapy (aPTT) are promising local and effective alternative therapies for antibiotic resistant bacterial infections and biofilms.

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