Effects of Dentin Surface Modifications Treated with Er:YAG and Nd:YAG Laser Irradiation on Fibroblast Cell Adhesion

2012 ◽  
Vol 30 (2) ◽  
pp. 63-70 ◽  
Author(s):  
Gombo Bolortuya ◽  
Arata Ebihara ◽  
Shizuko Ichinose ◽  
Satoshi Watanabe ◽  
Tomoo Anjo ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Laser Irradiation ◽  
Nd:Yag Laser ◽  
Surface Modifications ◽  
Fibroblast Cell ◽  
Dentin Surface

Effect of Nd:YAG Laser Irradiation Pretreatment on the Long-Term Bond Strength of Etch-and-Rinse Adhesive to Dentin

2017 ◽  
Vol 42 (1) ◽  
pp. 62-72 ◽  
Author(s):  
J Gan ◽  
S Liu ◽  
L Zhou ◽  
Y Wang ◽  
J Guo ◽  
...  
Keyword(s):  
Bond Strength ◽  
Laser Irradiation ◽  
Nd:Yag Laser ◽  
Knoop Hardness ◽  
Control Group ◽  
In Vitro Test ◽  
Dentin Surface ◽  
Etch And Rinse ◽  
Significant Difference

SUMMARY Purpose: To investigate the effect of neodymium-doped yttrium aluminum garnet (Nd:YAG) laser irradiation pretreatment on the long-term bond strength of an etch-and-rinse adhesive to dentin. Methods: Fifty molars were sectioned parallel to the occlusal plane and randomly divided into two groups (n=25 per group): control group (no treatment) and laser group (dentin surfaces were treated with Nd:YAG laser at a setting of 100 mJ/10 Hz). Afterward, resin was bonded to the dentin surface using a two-step etch-and-rinse adhesive (Adper SingleBond 2), and then 150 beams of each group were produced. Each group was divided into three subgroups (n=50 each group): 24 hours of water storage, thermocycling, and NaOCl storage. The microtensile bond strength (MTBS), failure modes, nanoleakage expression, and Masson's trichrome staining were evaluated. An additional 20 molars were sectioned to obtain 2-mm-thick flat dentin slices. These slices were randomly divided into control and laser-treated groups as mentioned previously. Then slices of each group were examined by scanning electron microscopy, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), and the Knoop hardness test. Results: The results of ATR-FTIR and Masson's trichrome verified that laser irradiation partly removed collagen fibers from the dentin surface; however, no significant difference was found in the Knoop hardness (p>0.05). The XRD result showed similar crystalline structure regardless of laser pretreatment. There is no significant difference in short-term MTBS between control and laser-treated groups (p>0.05); however, long-term MTBS differed between the groups (p>0.05). Furthermore, the laser-treated group showed less silver deposition than the control group after aging (p<0.05). Conclusion: Pretreatment by Nd:YAG laser irradiation appeared to have a positive effect on the adhesive-dentin bonding in vitro test, and the bonding effectiveness could be preserved after aging.

Surface modifications induced by nanosecond pulsed Nd:YAG laser irradiation of metallic substrates

2006 ◽  
Vol 201 (3-4) ◽  
pp. 1383-1392 ◽  
Author(s):  
H. Li ◽  
S. Costil ◽  
V. Barnier ◽  
R. Oltra ◽  
O. Heintz ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Nd:Yag Laser ◽  
Surface Modifications ◽  
Pulsed Nd:Yag Laser ◽  
Metallic Substrates

Investigation of Au SAMs Photoclick Derivatization by PM-IRRAS

2019 ◽  
Author(s):  
Mark Workentin ◽  
François Lagugné-Labarthet ◽  
Sidney Legge
Keyword(s):  
Cell Adhesion ◽  
Self Assembly ◽  
Materials Science ◽  
Fibroblast Cell ◽  
Fine Tuning ◽  
Chemical System ◽  
Infrared Reflection ◽  
Assembled Monolayers ◽  
One Step ◽  
High Degree

In this work we present a clean one-step process for modifying headgroups of self-assembled monolayers (SAMs) on gold using photo-enabled click chemistry. A thiolated, cyclopropenone-caged strained alkyne precursor was first functionalized onto a flat gold substrate through self-assembly. Exposure of the cyclopropenone SAM to UV-A light initiated the efficient photochemical decarbonylation of the cyclopropenone moiety, revealing the strained alkyne capable of undergoing the interfacial strain-promoted alkyne-azide cycloaddition (SPAAC). Irradiated SAMs were derivatized with a series of model azides with varied hydrophobicity to demonstrate the generality of this chemical system for the modification and fine-tuning of the surface chemistry on gold substrates. SAMs were characterized at each step with polarization-modulation infrared reflection-absorption spectroscopy (PM-IRRAS) to confirm successful functionalization and reactivity. Furthermore, to showcase the compatibility of this approach with biochemical applications, cyclopropenone SAMs were irradiated and modified with azide-bearing cell adhesion peptides to promote human fibroblast cell adhesion, then imaged by live cell fluorescence microscopy. Thus, the “photoclick” methodology reported here represents an improved, versatile, catalyst-free protocol that allows for a high degree of control over the modification of material surfaces, with applicability in materials science as well as biochemistry.<br>

Preventive Effect of Tooth Fracture by Pulsed Nd:YAG Laser Irradiation with Diamine Silver Fluoride Solution

2001 ◽  
Vol 19 (6) ◽  
pp. 315-318 ◽  
Author(s):  
Keiko Yokoyama ◽  
Yuichi Kimura ◽  
Koukichi Matsumoto ◽  
Akihiro Fujishima ◽  
Takashi Miyazaki
Keyword(s):  
Laser Irradiation ◽  
Nd:Yag Laser ◽  
Preventive Effect ◽  
Fluoride Solution ◽  
Pulsed Nd:Yag Laser ◽  
Tooth Fracture ◽  
Silver Fluoride

Influence of Nd:YAG Laser Irradiation on an Adhesive Restorative Procedure

2006 ◽  
Vol 31 (5) ◽  
pp. 604-609 ◽  
Author(s):  
M. Franke ◽  
A. W. Taylor ◽  
A. Lago ◽  
M. C. Fredel
Keyword(s):  
Statistical Analysis ◽  
Bond Strength ◽  
Laser Irradiation ◽  
Significant Influence ◽  
Nd:Yag Laser ◽  
Deleterious Effect ◽  
Adhesive System ◽  
High Energy ◽  
Low Energy ◽  
Adhesive Penetration

Clinical Relevance Statistical analysis of the results obtained in this study shows that Nd:YAG laser irradiation on the adhesive system has a significant influence on bond strength to dentin. Bond strength is improved by better adhesive penetration when low energy is applied; whereas, high energy densities have a deleterious effect on the procedure.

Effect of Pre-Oxidized CuO Layer in Joining between Polyethylene Terephalate (PET) and Copper (Cu) by Using Pulsed Nd:YAG Laser

2010 ◽  
Vol 129-131 ◽  
pp. 714-718 ◽  
Author(s):  
Farazila Yusof ◽  
Yoshiharu Mutoh ◽  
Yukio Miyashita
Keyword(s):  
Laser Irradiation ◽  
Nd:Yag Laser ◽  
Shear Failure ◽  
High Heat ◽  
Experimental Result ◽  
Joint Interface ◽  
Intimate Contact ◽  
Cross Sectional ◽  
Pulsed Nd:Yag Laser ◽  
Oxide Particles

In the present study, effect of pre-oxidized (PO) CuO layer in the joining between polyethylene terephalate (PET) and copper (Cu) by using pulsed Nd:YAG laser was investigated. The experimental result for PET/PO Cu (with pre-oxidized layer) and PET/Cu (without pre-oxidized layer) were presented in this paper. The results showed the pre-oxidation surface improved the welding efficiency. The welded area and tensile shear failure load (TSFL) of PET/PO Cu joint were increased when pre-oxidized layer was introduced. This indicates that high heat was absorbed during laser irradiation of PET/PO-Cu joint compare to PET/Cu joint. On the other hand, the fracture surface of PET/PO-Cu showed the presence of Cu and Cu oxide particles in the PET side. While in case of PET/Cu, no occurrence of Cu particles can be observed. The presence of Cu oxide particles and Cu particles were believed may increase PET/PO Cu joint shear force. From the cross-sectional observation of PET/PO Cu joint interface, it was found that an oxide layer in the laser irradiation area was removed and PET seemed to have intimate contact with Cu surface.

Impact of Pulsed Nd:YAG Laser Irradiation on the Growth and Mortality of the Biofilm Forming Marine Bacterium Pseudoalteromonas carrageenovora

Biofouling ◽  
2002 ◽  
Vol 18 (2) ◽  
pp. 123-127 ◽  
Author(s):  
Kanavillil Nandakumar ◽  
Hideki Obika ◽  
Tatsuya Shinozaki ◽  
Toshihiko Ooie ◽  
Akihiro Utsumi ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Nd:Yag Laser ◽  
Marine Bacterium ◽  
Pulsed Nd:Yag Laser

The effect of a pulsed-Nd:YAG laser irradiation on microstructure of human gingiva

1997 ◽  
Vol 27 (2) ◽  
pp. 317
Author(s):  
Kyung-Yoon Han ◽  
Kwang-Yong Shin ◽  
Chun-Seok Kim ◽  
Hyung-Soo Kim ◽  
Chang-Yup Yum ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Nd:Yag Laser ◽  
Pulsed Nd:Yag Laser ◽  
Human Gingiva

Dentin Evaluation after Nd:YAG Laser Irradiation Using Short and Long Pulses

2004 ◽  
Vol 22 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Eduardo H. Moriyama ◽  
Renato A. Zângaro ◽  
Antonio Balbin Villaverde ◽  
Paulo D.C. Lobo ◽  
Egberto Munin ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Nd:Yag Laser
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