Prediction of Epitopes in Hemagglutinin and Neuraminidase Proteins of Influenza A Virus H5N1 Strain: A Clue for Diagnostic and Vaccine Development

2008 ◽  
Vol 12 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Pallavi Somvanshi ◽  
Vijay Singh ◽  
P.K. Seth
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Vaccine Development ◽  
Virus H5n1 ◽  
H5n1 Strain

Broad neutralization of H1 and H3 viruses by adjuvanted influenza HA stem vaccines in nonhuman primates

2021 ◽  
Vol 13 (583) ◽  
pp. eabe5449
Author(s):  
Nicole Darricarrère ◽  
Yu Qiu ◽  
Masaru Kanekiyo ◽  
Adrian Creanga ◽  
Rebecca A. Gillespie ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Nonhuman Primates ◽  
Vaccine Development ◽  
Protective Efficacy ◽  
Neutralizing Antibody ◽  
Gene Families ◽  
Influenza Viruses ◽  
Public Health Importance ◽  
Water Emulsion

Seasonal influenza vaccines confer protection against specific viral strains but have restricted breadth that limits their protective efficacy. The H1 and H3 subtypes of influenza A virus cause most of the seasonal epidemics observed in humans and are the major drivers of influenza A virus–associated mortality. The consequences of pandemic spread of COVID-19 underscore the public health importance of prospective vaccine development. Here, we show that headless hemagglutinin (HA) stabilized-stem immunogens presented on ferritin nanoparticles elicit broadly neutralizing antibody (bnAb) responses to diverse H1 and H3 viruses in nonhuman primates (NHPs) when delivered with a squalene-based oil-in-water emulsion adjuvant, AF03. The neutralization potency and breadth of antibodies isolated from NHPs were comparable to human bnAbs and extended to mismatched heterosubtypic influenza viruses. Although NHPs lack the immunoglobulin germline VH1-69 residues associated with the most prevalent human stem-directed bnAbs, other gene families compensated to generate bnAbs. Isolation and structural analyses of vaccine-induced bnAbs revealed extensive interaction with the fusion peptide on the HA stem, which is essential for viral entry. Antibodies elicited by these headless HA stabilized-stem vaccines neutralized diverse H1 and H3 influenza viruses and shared a mode of recognition analogous to human bnAbs, suggesting that these vaccines have the potential to confer broadly protective immunity against diverse viruses responsible for seasonal and pandemic influenza infections in humans.

scholarly journals Mutations in Influenza A Virus (H5N1) and Possible Limited Spread, Turkey, 2006

2008 ◽  
Vol 14 (3) ◽  
pp. 491-492 ◽  
Author(s):  
Ender Altiok ◽  
Fulya Taylan ◽  
Osman Ş. Yenen ◽  
Gülşah Demirkeser ◽  
Mürvet Bozaci ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Virus H5n1

scholarly journals Protection and Virus Shedding of Falcons Vaccinated against Highly Pathogenic Avian Influenza A Virus (H5N1)

2007 ◽  
Vol 13 (11) ◽  
pp. 1667-1674 ◽  
Author(s):  
Michael Lierz ◽  
Hafez M. Hafez ◽  
Robert Klopfleisch ◽  
Dörte Lüschow ◽  
Christine Prusas ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Influenza A Virus ◽  
Influenza A ◽  
Highly Pathogenic Avian Influenza ◽  
Highly Pathogenic ◽  
Virus Shedding ◽  
Pathogenic Avian Influenza ◽  
Virus H5n1 ◽  
Avian Influenza A Virus

Construction and packaging of Semliki forest virus replicon particles efficiently expressing Influenza A virus (H5N1) hemagglutinin

2009 ◽  
Vol 53 (2) ◽  
pp. 139-141 ◽  
Author(s):  
S. Yang ◽  
J. Wo ◽  
M. Li ◽  
C. Yu ◽  
G. Lv ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Semliki Forest Virus ◽  
Virus H5n1

scholarly journals Influenza A Virus (H5N1) Infection in Cats Causes Systemic Disease with Potential Novel Routes of Virus Spread within and between Hosts

2006 ◽  
Vol 168 (1) ◽  
pp. 176-183 ◽  
Author(s):  
Guus F. Rimmelzwaan ◽  
Debby van Riel ◽  
Marianne Baars ◽  
Theo M. Bestebroer ◽  
Geert van Amerongen ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Systemic Disease ◽  
Virus Spread ◽  
H5n1 Infection ◽  
Virus H5n1

scholarly journals Epidemiology of the Influenza A Virus H5N1 Subtype and Memory of Immunity to the H2N2 Subtype

mBio ◽  
2012 ◽  
Vol 3 (4) ◽  
Author(s):  
Masanori Terajima ◽  
Jenny Aurielle B. Babon ◽  
Francis A. Ennis
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
H5n1 Subtype ◽  
Virus H5n1

scholarly journals Vaccination of Macaques with Adjuvanted Formalin-Inactivated Influenza A Virus (H5N1) Vaccines: Protection against H5N1 Challenge without Disease Enhancement

2007 ◽  
Vol 82 (5) ◽  
pp. 2565-2569 ◽  
Author(s):  
Caroline Ruat ◽  
Catherine Caillet ◽  
Alexandre Bidaut ◽  
James Simon ◽  
Albert D. M. E. Osterhaus
Keyword(s):  
Influenza A Virus ◽  
Measles Virus ◽  
Influenza A ◽  
Upper Respiratory Tract ◽  
Disease Exacerbation ◽  
Water Emulsion ◽  
Virus H5n1 ◽  
Oil In Water ◽  
Syncytial Virus ◽  
Oil In Water Emulsion

ABSTRACT We investigated the ability of adjuvanted, inactivated split-virion influenza A virus (H5N1) vaccines to protect against infection and demonstrated that the disease exacerbation phenomenon seen with adjuvanted formaldehyde-inactivated respiratory syncytial virus and measles virus investigational vaccines did not occur with these H5N1 vaccines. Macaques were vaccinated twice with or without an aluminum hydroxide or oil-in-water emulsion adjuvanted vaccine. Three months later, animals were challenged with homologous wild-type H5N1. No signs of vaccine-induced disease exacerbation were seen. With either adjuvant, vaccination induced functional and cross-reactive antibodies and protected the lungs and upper respiratory tract. Without an adjuvant, the vaccine provided partial protection. Best results were obtained with the emulsion adjuvant.

scholarly journals Activation of Tumor Antigen-Specific Cytotoxic T Lymphocytes (CTLs) by Human Dendritic Cells Infected with an Attenuated Influenza A Virus Expressing a CTL Epitope Derived from the HER-2/neu Proto-Oncogene

2003 ◽  
Vol 77 (13) ◽  
pp. 7411-7424 ◽  
Author(s):  
Clay L. Efferson ◽  
Jeanne Schickli ◽  
Byung Kyum Ko ◽  
Kouichiro Kawano ◽  
Sara Mouzi ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Influenza A Virus ◽  
Tumor Antigen ◽  
Influenza A ◽  
Vaccine Development ◽  
Interleukin 2 ◽  
Transduction Efficiency ◽  
Ctl Epitope ◽  
The Poor ◽  
Her 2

ABSTRACT The development of cancer vaccines requires approaches to induce expansion and functional differentiation of tumor antigen-specific cytotoxic T lymphocyte (CTL) effectors which posses cytolytic capability and produce cytokines. Efficient induction of such cells is hindered by the poor immunogenicity of tumor antigens and by the poor transduction efficiency of dendritic cells (DCs) with current nonreplicating vectors. We have investigated the use of influenza A virus, a potent viral inducer of CTLs, as a vector expressing the immunodominant HER-2 CTL epitope KIF (E75). For this purpose, an attenuated influenza A/PR8/34 virus with a truncated nonstructural (NS1) gene was generated containing the E75 epitope in its neuraminidase protein (KIF-NS virus). Stimulation of peripheral blood mononuclear cells from healthy donors and of tumor-associated lymphocytes from ovarian and breast cancer patients with DCs infected with KIF-NS virus (KIF-NS DC) induced CTLs that specifically recognized the peptide KIF and HER-2-expressing tumors in cytotoxicity assays and secreted gamma interferon (IFN-γ) and interleukin-2 at recall with peptide. Priming with KIF-NS DCs increased the number of E75+ CD45RO+ cells by more than 10-fold compared to nonstimulated cells. In addition, KIF-NS virus induced high levels of IFN-α in DCs. This is the first report demonstrating induction of human epitope-specific CTLs against a tumor-associated antigen with a live attenuated recombinant influenza virus vector. Such vectors may provide a novel approach for tumor antigen delivery, lymphocyte activation, and differentiation in human cancer vaccine development.

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