Gene Silencing Activity and Hepatic Accumulation of Antisense Oligonucleotides Bearing Cholesterol-Conjugated Thiono Triester at the Gap Region

2017 ◽  
Vol 27 (4) ◽  
pp. 232-237 ◽  
Author(s):  
Mado Nakajima ◽  
Takeshi Kasuya ◽  
Shinnichi Yokota ◽  
Reina Onishi ◽  
Tatsuya Ikehara ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Antisense Oligonucleotides

scholarly journals High-Content Imaging Analysis of the Knockdown Effects of Validated siRNAs and Antisense Oligonucleotides

2007 ◽  
Vol 12 (6) ◽  
pp. 775-788 ◽  
Author(s):  
Jonathan Low ◽  
Shuguang Huang ◽  
Michele Dowless ◽  
Wayne Blosser ◽  
Thomas Vincent ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Gene Silencing ◽  
Antisense Oligonucleotides ◽  
Complex Data ◽  
Distribution Analysis ◽  
Phenotypic Analysis ◽  
M Cell ◽  
High Content Imaging ◽  
Cellular Effects ◽  
Complex Data Sets

High-content imaging (HCI) provides researchers with a powerful tool for understanding cellular processes. Although phenotypic analysis generated through HCI is a potent technique to determine the overall cellular effects of a given treatment, it frequently produces complex data sets requiring extensive interpretation. The authors developed statistical analyses to decrease the time spent to determine the outcome of each HCI assay and to better understand complex phenotypic changes. To test these tools, the authors performed a comparison experiment between 2 types of oligonucleotide-mediated gene silencing (OMGS), antisense oligonucleotides (ASOs), and short, double-stranded RNAs (siRNAs). Although similar in chemical structure, these 2 methods differ in cellular mechanism of action and off-target effects. Using a library of 50 validated ASOs and siRNAs to the same targets, the authors characterized the differential effects of these 2 technologies using a HeLa cell G2-M cell cycle assay. Although knockdown of a variety of targets by ASOs or siRNAs affected the cell cycle profile, few of those targets were affected by both ASOs and siRNAs. Distribution analysis of population changes induced through target knockdown led to the identification of targets that, when inhibited, could affect the G2-M transition in the cell cycle in a statistically significant manner. The distinctly different mechanisms of action of these 2 forms of gene silencing may help define the use of these treatments in both clinical and research environments. ( Journal of Biomolecular Screening 2007:775-788)

Gapmer Antisense Oligonucleotides Containing 2′,3′‐Dideoxy‐2′‐fluoro‐3′‐ C ‐hydroxymethyl‐β‐ d ‐lyxofuranosyl Nucleotides Display Site‐Specific RNase H Cleavage and Induce Gene Silencing

2020 ◽  
Vol 26 (6) ◽  
pp. 1368-1379
Author(s):  
Mathias B. Danielsen ◽  
Chenguang Lou ◽  
Jolanta Lisowiec‐Wachnicka ◽  
Anna Pasternak ◽  
Per T. Jørgensen ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Antisense Oligonucleotides ◽  
Rnase H ◽  
Site Specific

scholarly journals 696. Pre-Clinical Evaluation of Allele-Specific Mutant Huntingtin Gene Silencing Antisense Oligonucleotides

2015 ◽  
Vol 23 ◽  
pp. S277
Author(s):  
Amber L. Southwell ◽  
Niels H. Skotte ◽  
Nicholas Caron ◽  
Holly Kordasiewicz ◽  
Michael Oestergaard ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Clinical Evaluation ◽  
Antisense Oligonucleotides ◽  
Mutant Huntingtin ◽  
Allele Specific

scholarly journals Hydrogen peroxide-triggered gene silencing in mammalian cells through boronated antisense oligonucleotides

2018 ◽  
Vol 9 (5) ◽  
pp. 1112-1118 ◽  
Author(s):  
Shohei Mori ◽  
Kunihiko Morihiro ◽  
Takumi Okuda ◽  
Yuuya Kasahara ◽  
Satoshi Obika
Keyword(s):  
Hydrogen Peroxide ◽  
Gene Silencing ◽  
Antisense Oligonucleotides ◽  
Mammalian Cells ◽  
Arylboronic Acid

Arylboronic acid-modified antisense oligonucleotides enable hydrogen peroxide induced gene silencing in mammalian cells.

scholarly journals Direct comparison of the specificity of gene silencing using antisense oligonucleotides and RNAi

2005 ◽  
Vol 388 (2) ◽  
pp. 573-583 ◽  
Author(s):  
Benoit BILANGES ◽  
David STOKOE
Keyword(s):  
Gene Silencing ◽  
Antisense Oligonucleotides ◽  
Expression Profiles ◽  
Specific Pattern ◽  
Affymetrix Microarrays ◽  
Short Time ◽  
Interfering Rna ◽  
First Time ◽  
Gene Alterations ◽  
Time Point

RNAi (RNA interference) and ASO (antisense oligonucleotide) technologies are the most commonly used approaches for silencing gene expression. However, the specificity of such powerful tools is an important factor to correctly interpret the biological consequences of gene silencing. In the present study, we examined the effects of acute loss of Ser/Thr kinase PDK1 (3-phosphoinositide-dependent kinase 1) expression using ASO and RNAi, and compared, for the first time, these two techniques using Affymetrix microarrays. We show that both ASO- and siRNA (small interfering RNA)-mediated knock-down of PDK1 expression strongly inhibited cell proliferation, although by different mechanisms, thereby questioning the specificity of these reagents. Using microarray analysis, we characterized the specificity of the ASO- and siRNA-mediated gene silencing of PDK1 by examining expression profiles 48 and 72 h following oligonucleotide transfection. At 48 h, a PDK1-dependent pattern of gene alterations was detectable, despite a large number of non-specific changes due to transfection of control nucleic acids. These non-specific alterations became more apparent at the 72 h time point, and obscured any PDK1-specific pattern. This study underscores the importance of defining appropriate control ASOs and siRNAs, using multiple oligonucleotides for each target and preferably short time points following transfection to avoid misinterpretation of the phenotype observed.

scholarly journals Efficient gene silencing by delivery of locked nucleic acid antisense oligonucleotides, unassisted by transfection reagents

2009 ◽  
Vol 38 (1) ◽  
pp. e3-e3 ◽  
Author(s):  
C. A. Stein ◽  
J. Bo Hansen ◽  
Johnathan Lai ◽  
SiJian Wu ◽  
Anatoliy Voskresenskiy ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Gene Silencing ◽  
Antisense Oligonucleotides ◽  
Locked Nucleic Acid ◽  
Efficient Gene
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