Antibiotic Resistance Patterns and mcr-1 Detection in Avian Pathogenic Escherichia coli Isolates from Commercial Layer and Layer Breeder Flocks Demonstrating Colibacillosis in Greece

2020 ◽  
Author(s):  
Dimitrios Koutsianos ◽  
Labrini V. Athanasiou ◽  
Tilemachos Dimitriou ◽  
Marios Nikolaidis ◽  
Christina Tsadila ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

scholarly journals Molecular Detection of Avian Pathogenic Escherichia coli (APEC) for the First Time in Layer Farms in Bangladesh and Their Antibiotic Resistance Patterns

2020 ◽  
Vol 8 (7) ◽  
pp. 1021 ◽  
Author(s):  
Samina Ievy ◽  
Md. Saiful Islam ◽  
Md. Abdus Sobur ◽  
Mithun Talukder ◽  
Md. Bahanur Rahman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Virulence Genes ◽  
Molecular Detection ◽  
Avian Pathogenic Escherichia Coli ◽  
Internal Organs ◽  
Pathogenic Escherichia Coli ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns ◽  
First Time

Avian pathogenic Escherichia coli (APEC) causes significant economic losses in poultry industries. Here, we determined for the first time in Bangladesh, the prevalence of APEC-associated virulence genes in E. coli isolated from layer farms and their antibiotic resistance patterns. A total of 99 samples comprising internal organs, feces, and air were collected from 32 layer farms. Isolation was performed by culturing samples on eosin–methylene blue agar plates, while the molecular detection of APEC was performed by PCR, and antibiograms were performed by disk diffusion. Among the samples, 36 were positive for the APEC-associated virulence genes fimC, iucD, and papC. Out of 36 isolates, 7, 18, and 11 were positive, respectively, for three virulence genes (papC, fimC, and iucD), two virulence genes, and a single virulence gene. Although the detection of virulence genes was significantly higher in the internal organs, the air and feces were also positive. The antibiograms revealed that all the isolates (100%) were resistant to ampicillin and tetracycline; 97.2%, to chloramphenicol and erythromycin; 55.5%, to enrofloxacin; 50.0%, to norfloxacin and ciprofloxacin; 19.4%, to streptomycin; 11.1%, to colistin; and 8.33%, to gentamicin. Interestingly, all the isolates were multidrug-resistant (MDR). Spearman’s rank correlation coefficient analysis revealed the strongest significant correlation between norfloxacin and ciprofloxacin resistance. This is the first study in Bangladesh describing the molecular detection of APEC in layer farms. Isolated APEC can now be used for detailed genetic characterization and assessing the impact on public health.

scholarly journals Antibiotic Resistance and Virulence Gene Patterns Associated with Avian Pathogenic Escherichia coli (APEC) from Broiler Chickens in Qatar

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 564
Author(s):  
Alreem Johar ◽  
Najlaa Al-Thani ◽  
Sara H. Al-Hadidi ◽  
Elyes Dlissi ◽  
Mahmoud H. Mahmoud ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Broiler Chickens ◽  
Virulence Genes ◽  
Virulence Gene ◽  
Avian Pathogenic Escherichia Coli ◽  
E Coli ◽  
Pathogenic Escherichia Coli ◽  
Special Programs ◽  
Resistance Patterns

Avian Pathogenic Escherichia coli (APEC) is the contributing agent behind the avian infectious disease colibacillosis, which causes substantial fatalities in poultry industries that has a significant impact on the economy and food safety. Several virulence genes have been shown to be concomitant with the extraintestinal survival of APEC. This study investigates the antibiotic resistance patterns and APEC-associated virulence genes content in Escherichia coli isolated from non-healthy and healthy broiler chickens from a commercial poultry farm in Qatar. A total of 158 E. coli strains were isolated from 47 chickens from five different organs (air sac, cloacal, kidney, liver, and trachea). Based on genetic criteria, 65% were APEC strains containing five or more virulence genes, and 34% were non-pathogenic E. coli (NPEC) strains. The genes ompT, hlyF, iroN, tsh, vat, iss, cvi/cva, and iucD were significantly prevalent in all APEC strains. E. coli isolates showed 96% resistance to at least one of the 18 antibiotics, with high resistance to ampicillin, cephalothin, ciprofloxacin, tetracycline, and fosfomycin. Our findings indicate high antibiotic resistance prevalence in non-healthy and healthy chicken carcasses. Such resistant E. coli can spread to humans. Hence, special programs are required to monitor the use of antibiotics in chicken production in Qatar.

Occurrence of Enteropathogenic Escherichia coli Serotypes in Butter

1987 ◽  
Vol 50 (10) ◽  
pp. 829-831 ◽  
Author(s):  
F. M. ABBAR ◽  
M. TAHIR MOHAMED
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Fecal Coliforms ◽  
Enteropathogenic Escherichia Coli ◽  
Heat Stable ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

A total of 30 samples of butter analysed during the course of the investigation showed that fecal coliforms were absent from only 13.3% of samples. One hundred forty colonies of fecal coliforms were biochemically characterized with the following types obtained (Escherichia sp. 41.4%, Enterobacter sp. 25.7%, Citrobacter sp. 20%, Klebsiella sp. 10%). Five different serotypes, namely 0 125 K70(2), 0 142K86(1), 0 127K63(1), 0 114 K90(2), 0 111 K58(1) were detected in 7 of 58 Escherichia coli isolates and 51 strains were untypable. Three strains produced heat stable (ST) enterotoxin and belonged to the enteropathogenic serotype. The antibiotic resistance patterns of coliform strains are presented.

scholarly journals Antibiotic Resistance Patterns of Diverse Escherichia coli Phylogenetic Groups Isolated from the Al-Hillah River in Babylon Province, Iraq

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Mourouge Saadi Alwash ◽  
Hawraa Mohammed Al-Rafyai
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Anthropogenic Activities ◽  
Health Concern ◽  
Resistant Bacteria ◽  
Anthropogenic Pressures ◽  
Phylogenetic Groups ◽  
E Coli ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

Surface water contamination remains a major worldwide public health concern and may contribute to the dissemination of antibiotic-resistant bacteria. The Al-Hillah River in the city of Babylon Province, Iraq, diverts flows from the Euphrates River. Because of its importance in irrigation and population density, it faces several forced and unforced changes due to anthropogenic activities. To evaluate water quality, water samples were collected from three sites with different anthropogenic pressures along the Al-Hillah River. These samples were subjected to bacteriological analyses, i.e., total coliforms, Escherichia coli, and faecal enterococci. The phylogenetic groups of the E. coli isolates (n = 61) were typed by rapid PCR-based analyses. Representatives of each isolate were tested phenotypically for resistance to six classes of antibiotics and characterized according to their phylogenetic groups. The results demonstrated the highest resistance levels were to β-lactam antibiotics, followed by fosfomycin and aminoglycosides. Escherichia coli isolates belonging to phylogenetic groups A and B2 were the most common and were characterized by a higher prevalence of antibiotic resistance. This study is important for understanding the current conditions of the Al-Hillah River, as the data reveal a high prevalence of multiresistance among E. coli isolates circulating at the three sampling sites.

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